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Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits Cover

Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits

Open Access
|Oct 2021

Figures & Tables

Figure 1

Surgical procedure. (A) Internal jugular vein, common carotid artery, and external jugular vein of rabbit. (B) Vein dilatated with hydraulic pressure. (C) Vein dilatated with hydraulic pressure (close-up). (D) Successfully constructed fistula.
Surgical procedure. (A) Internal jugular vein, common carotid artery, and external jugular vein of rabbit. (B) Vein dilatated with hydraulic pressure. (C) Vein dilatated with hydraulic pressure (close-up). (D) Successfully constructed fistula.

Figure 2

Histology. (A) Venous wall before dilation. (B) Venous wall after dilation. (C) Changes of fistula vein in control group 3 weeks after operation. (D) changes of fistula vein in hydraulic expansion group 3 weeks after operation (all panels HE staining, ×200; scale bars represent 50 μm). HE, hematoxylin and eosin.
Histology. (A) Venous wall before dilation. (B) Venous wall after dilation. (C) Changes of fistula vein in control group 3 weeks after operation. (D) changes of fistula vein in hydraulic expansion group 3 weeks after operation (all panels HE staining, ×200; scale bars represent 50 μm). HE, hematoxylin and eosin.

Figure 3

Expression of TGF-β1 immunoreactivity in vein (A) before surgery, (B) fistula vein in control group 3 weeks after surgery, (C) fistula vein in hydraulic expansion group 3 weeks after surgery. Expression of CTGF immunoreactivity in vein (D) before surgery, (E) fistula vein in control group 3 weeks after surgery, (F) fistula vein in hydraulic expansion group 3 weeks after surgery. (DAB staining of immunocomplexes, ×200; scale bars represent 50 μm). CTGF, connective tissue growth factor; DAB, diaminobenzidine; TGF-β1, transforming growth factor-β1.
Expression of TGF-β1 immunoreactivity in vein (A) before surgery, (B) fistula vein in control group 3 weeks after surgery, (C) fistula vein in hydraulic expansion group 3 weeks after surgery. Expression of CTGF immunoreactivity in vein (D) before surgery, (E) fistula vein in control group 3 weeks after surgery, (F) fistula vein in hydraulic expansion group 3 weeks after surgery. (DAB staining of immunocomplexes, ×200; scale bars represent 50 μm). CTGF, connective tissue growth factor; DAB, diaminobenzidine; TGF-β1, transforming growth factor-β1.

Diameter of fistula vein after AVF surgery in rabbits

Postoperative dayHydraulic expansion (n = 6)Diameter (mm)Control (n = 6)Diameter (mm)FP
Baseline (0)4.34 ± 0.502.80 ± 0.576.790.02*
    14.02 ± 0.583.01 ± 0.187.870.03*
    23.92 ± 0.913.34 ± 0.260.970.53
    35.12 ± 1.304.36 ± 0.861.720.28
    74.93 ± 0.524.17 ± 0.511.940.22
    145.19 ± 1.044.18 ± 0.520.990.51
    214.52 ± 0.294.39 ± 0.280.950.52

Baseline data for rabbits in experimental and control groups_

Hydraulic expansion (n = 6)Control (n = 6)
Weight (kg)2.2 ± 0.12.26 ± 0.11
Dose 1% pentobarbital (mL)8.67 ± 1.447.38 ± 0.88
Operating time (min)150 ± 20*84 ± 20.8
Artery diameter, preoperative (mm)1.73 ± 0.181.8 ± 0.21
Vein diameter, preoperative (mm)2.73 ± 0.512.48 ± 0.65

Intimal thickness and immunohistochemistry score 3 weeks after surgery

Hydraulic expansion (n = 6)Control (n = 6)F4,11P
Intimal thickness (mm)39.20 ± 3.7839.34 ± 1.910.950.51
Expression of TGF-β1 (score)4.32 ± 0.315.32 ± 0.910.600.71
Expression of CTGF (score)3.70 ± 0.054.90 ± 0.097.870.01*
DOI: https://doi.org/10.2478/abm-2021-0028 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Language: English
Page range: 223 - 232
Published on: Oct 29, 2021
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year

© 2021 Wanjun Ren, Jiyuan Niu, Yuejuan Du, Huili Jiang, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.