
Genotoxicity of Metal Nanoparticles: Focus on In Vivo Studies
By: Katharina Klien and Jasminka Godnić-Cvar
Open Access
|Jun 2012References
- Sosnovik DE, Nahrendorf M, Weissleder R. Magnetic nanoparticles for MR imaging: agents, techniques and cardiovascular applications. Basic Res Cardiol 2008;103:122-30.
- Corchero J, Villaverde A. Biomedical applications of distally controlled magnetic nanoparticles. Trends Biotechnol 2009;27:468-76.
- Luther W. Industrial application of nanomaterials - chances and risks. Future Technologies 2004:54;1-112.
- European Agency for Safety and Health at Work (EU-OSHA). Workplace exposure to nanoparticles 2009 [displayed 29 Jan 2012]. Available at
- Singh N, Manshian B, Jenkins G, Griffiths S, Williams P, Maffeis T, Wright C, Doak S. NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials 2009;30:3891-914.
- Oberdörster G, Maynard A, Donaldson K, Castranova V, Fitzpatrick J, Ausman K, Carter J, Karn B, Kreyling W, Lai D, Olin S, Monteiro-Riviere N, Warheit D, Yang H; ILSI Research Foundation/Risk Science Institute Nanomaterial Toxicity Screening Working Group. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Part Fibre Toxicol 2005;2:8.
- Schatz G. Using theory and computation to model nanoscale properties. Proc Natl Acad Sci USA 2007;104:6885-92.
- Brouwer D. Exposure to manufactured nanoparticles in different workplaces. Toxicology 2010;269:120-7.
- Food and drug administration (FDA). Sunscreen Drug Products for Over-the-Counter Human Use. Amendment to the Tentative Final Monograph; Enforcement Policy. Federal Register /Vol. 63, No. 204/1998 [displayed 29 Jan 2012]. Available at
- Nakagawa Y, Wakuri S, Sakamoto K, Tanaka N. The photogenotoxicity of titanium dioxide particles. Mutat Res 1997;394:125-32.
- Wang JJ, Sanderson BJ, Wang H. Cyto- and genotoxicity of ultrafine TiOparticles in cultured human lymphoblastoid cells. Mutat Res 2007;628:99-106.
- Gurr JR, Wang AS, Chen CH, Jan KY. Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology 2005;213:66-73.
- Falck G, Lindberg H, Suhonen S, Vippola M, Vanhala E, Catalán J, Savolainen K, Norppa H. Genotoxic effects of nanosized and fine TiO2. Hum Exp Toxicol 2009;28:339-52.
- Borm P, Robbins D, Haubold S, Kuhlbusch T, Fissan H, Donaldson K, Schins R, Stone V, Kreyling W, Lademann J, Krutmann J, Warheit D, Oberdorster E. The potential risks of nanomaterials: a review carried out for ECETOC. Part Fibre Toxicol 2006;3:11.
- Snyder M, Lee J, Davis T, Scriven L, Tsapatsis M. Silica nanoparticle crystals and ordered coatings using lys-sil and a novel coating device. Langmuir 2007;23:9924-8.
- Ostiguy C, Lapointe G, Ménard L, Cloutier Y, Trottier M, Boutin M, Antoun M, Normand C. Nanoparticles. Actual knowledge about occupational health and saftey risks and prevention measures. Studies and Research Projects. Report R-470. Montréal: IRSST; 2006.
- Hong SC, Lee JH, Lee J, Kim HY, Park JY, Cho J, Han DW. Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups. Int J Nanomedicine 2011;6:3219-31.
- Ahamed M, Karns M, Goodson M, Rowe J, Hussain SM, Schlager JJ, Hong Y. DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. Toxicol Appl Pharmacol 2008;233:404-10.
- Sharma V, Singh SK, Anderson D, Tobin DJ, Dhawan A. Zinc oxide nanoparticle induced genotoxicity in primary human epidermal keratinocytes. J Nanosci Nanotechnol 2011;11:3782-8.
- Papageorgiou I, Brown C, Schins R, Singh S, Newson R, Davis S, Fisher J, Ingham E, Case CP. The effect of nano- and micron-sized particles of cobalt-chromium alloy on human fibroblasts. Biomaterials 2007;28:2946-58.
- Bhabra G, Sood A, Fisher B, Cartwright L, Saunders M, Evans WH, Surprenant A, Lopez-Castejon G, Mann S, Davis SA, Hails LA, Ingham E, Verkade P, Lane J, Heesom K, Newson R, Case CP. Nanoparticles can cause DNA damage across a cellular barrier. Nat Nanotechnol 2009;4:876-83.
- Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 2004;26:249-61.
- Fenech M. The micronucleus assay determination of chromosomal level DNA damage. Methods Mol Biol 2008;410:185-216.
- Trouiller B, Reliene R, Westbrook A, Solaimani P, Schiestl RH. Titanium dioxide nanoparticles induce DNA damage and genetic instabilityin mice. Cancer Res 2009;69:8784-9.
- Sycheva LP, Zhurkov VS, Iurchenko VV, Daugel-Dauge NO, Kovalenko MA, Krivtsova EK, Durnev AD. Investigation of genotoxic and cytotoxic effects of micro- and nanosized titanium dioxide in six organs of mice. Mutat Res 2011;726:8-14.
- Saber AT, Jacobsen NR, Mortensen A, Szarek J, Jackson P, Madsen AM, Jensen KA, Koponen IK, Brunborg G, Gützkow KB, Vogel U, Wallin H. Nanotitanium dioxide toxicity in mouse lung is reduced in sanding dust from paint. Part Fibre Toxicol 2012;9:4.
- Landsiedel R, Ma-Hock L, Van Ravenzwaay B, Schulz M, Wiench K, Champ S, Schulte S, Wohlleben W, Oesch F. Gene toxicity studies on titanium dioxide and zinc oxide nanomaterials used for UV-protection in cosmetic formulations. Nanotoxicology 2010;4:364-81.
- Naya M, Kobayashi N, Ema M, Kasamoto S, Fukumuro M, Takami S, Nakajima M, Hayashi M, Nakanishi J.genotoxicity study of titanium dioxide nanoparticles using comet assay following intratracheal instillation in rats. Regul Toxicol Pharmacol 2011;62:1-6.
- Sharma V, Singh P, Pandey AK, Dhawan A. Induction of oxidative stress, DNA damage and apoptosis in mouse liver after sub-acute oral exposure to zinc oxide nanoparticles. Mutat Res 2012;745:84-91.
- Jiang H, Liu F, Yang H, Li Y. Effects of cobalt nanoparticles on human T cells. Biol Trace Elem Res 2012;146:23-9.
- Puntes VF, Krishnan KM, Alivisatos AP. Colloidal nanocrystal shape and size control: the case of cobalt. Science 2001;291:2115-7.
- Skumryev V, Stoyanov S, Zhang Y, Hadjipanayis G, Givord D, Nogués J. Beating the superparamagnetic limit with exchange bias. Nature 2003;423:850-3.
- Hwang dW, Lee DS, Kim S. Gene expression profiles for genotoxic effects of silica-free and silica-coated cobalt ferrite nanoparticles. J Nucl Med 2012;53:106-12.
- Girgis E, Khalil WK, Emam AN, Mohamed MB, Rao KV. Nanotoxicity of gold and gold-cobalt nanoalloy. Chem Res Toxicol 2012;25:1086-98.
- Li WR, Xie XB, Shi QS, Duan SS, Ouyang YS, Chen YB. Antibacterial effect of silver nanoparticles on. Biometals 2011;24:135-41.
- Tiwari DK, Jin T, Behari J. Dose-dependenttoxicity assessment of silver nanoparticle in Wistar rats. Toxicol Mech Methods 2011;21:13-24.
- Choi JE, Kim S, Ahn JH, Youn P, Kang JS, Park K, Yi J, Ryu DY. Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish. Aquat Toxicol 2010;100:151-9.
- Ahamed M, Posgai R, Gorey TJ, Nielsen M, Hussain SM, Rowe JJ. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol Appl Pharmacol 2010;242:263-9.
- Kim YS, Kim JS, Cho HS, Rha DS, Kim JM, Park JD, Choi BS, Lim R, Chang HK, Chung YH, Kwon IH, Jeong J, Han BS, Yu IJ. Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhal Toxicol 2008;20:575-83.
- Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V. A review of theandtoxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol 2010;40:328-46.
- Schulz M, Ma-Hock L, Brill S, Strauss V, Treumann S, Gröters S, van Ravenzwaay B, Landsiedel R. Investigation on the genotoxicity of different sizes of gold nanoparticles administered to the lungs of rats. Mutat Res 2011;745:51-7.
- Balasubramanyam A, Sailaja N, Mahboob M, Rahman MF, Misra S, Hussain SM, Grover P. Evaluation of genotoxic effects of oral exposure to aluminum oxide nanomaterials in rat bone marrow. Mutat Res 2009;676:41-7.
- Balasubramanyam A, Sailaja N, Mahboob M, Rahman MF, Hussain SM, Grover P.genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and micronucleus test. Mutagenesis 2009;24:245-51.
- Kang GS, Gillespie PA, Gunnison A, Moreira AL, Tchou-Wong KM, Chen LC. Long-term inhalation exposure to nickel nanoparticles exacerbated atherosclerosis in a susceptible mouse model. Environ Health Perspect 2011;119:176-81.
- Saber AT, Jensen KA, Jacobsen NR, Birkedal R, Mikkelsen L, Møller P, Loft S, Wallin H, Vogel U. Inflammatory and genotoxic effects of nanoparticles designed for inclusion in paints and lacquers. Nanotoxicology 2011 [Epub ahead of print]
- Organisation for Economics Co-operation and Development (OECD). Guidance Manual for the Testing of Manufactured Nanomaterials. 2010 [displayed 29 Jan 2012]. Available at
- National Institute for Occupational Safety and Health (NIOSH). Approaches to Safe Nanotechnology. Managing the Health and Safety Concerns Associated with Engineered Nanomaterials. 2009 [displayed 29 Jan 2012]. Available at
- Institut de recherche Robert-Sauvé en santé et en sécurité du travail (IRSST). Chemical Substances and Biological Agents, Studies and Research Projects, Report R-599. Best Practices Guide to Synthetic Nanoparticle Risk Management. 2009 [displayed 30 Jan 2012]. Available at
- Schulte PA, Mundt DJ, Nasterlack M, Mulloy KB, Mundt KA. Exposure registries: overview and utility for nanomaterial workers. J Occup Environ Med 2011;53(Suppl 6):S42-7.
DOI: https://doi.org/10.2478/10004-1254-63-2012-2213 | Journal eISSN: 1848-6312 (formerly 0004-1254) | Journal ISSN: 0004-1254
Language: English, Croatian
Page range: 133 - 145
Published on: Jun 21, 2012
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Related subjects:
© 2012 Katharina Klien, Jasminka Godnić-Cvar, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons License.