Biodegradation of Olive Mill Wastewater by Trichosporon Cutaneum and Geotrichum Candidum

Dragičević, Tibela; Hren, Marijana; Gmajnić, Margareta; Pelko, Sanja; Kungulovski, Dzoko; Kungulovski, Ivan; Čvek, Domagoj; Frece, Jadranka; Markov, Ksenija; Delaš, Frane

doi:10.2478/10004-1254-61-2010-2079

Abstract

Olive oil production generates large volumes of wastewater. These wastewaters are characterised by high chemical oxygen demand (COD), high content of microbial growth-inhibiting compounds such as phenolic compounds and tannins, and dark colour. The aim of this study was to investigate biodegradation of olive mill wastewater (OMW) by yeasts Trichosporon cutaneum and Geotrichum candidum. The yeast Trichosporon cutaneum was used because it has a high potential to biodegrade phenolic compounds and a wide range of toxic compounds. The yeast Geotrichum candidum was used to see how successful it is in biodegrading compounds that give the dark colour to the wastewater. Under aerobic conditions, Trichosporon cutaneum removed 88 % of COD and 64 % of phenolic compounds, while the dark colour remained. Geotrichum candidum grown in static conditions reduced COD and colour further by 77 % and 47 %, respectively. This investigation has shown that Trichosporon cutaneum under aerobic conditions and Geotrichum candidum under facultative anaerobic conditions could be used successfully in a two-step biodegradation process. Further investigation of OMW treatment by selected yeasts should contribute to better understanding of biodegradation and decolourisation and should include ecotoxicological evaluation of the treated OMW.

References

Hamdi M. Future prospects and constraints of olive mill wastewaters use and treatments: a review. Bioprocess Eng 1993;8:209-14.10.1007/BF00369831
Search in Google Scholar
Martin A, Borja R, Garcia I, Fiestas JA. Kinetics of methane production from olive mill wastewater. Process Biochem 1991;26:101-7.10.1016/0032-9592(91)80024-J
Search in Google Scholar
Ayed L, Hamdi M. Fermentative decolorization of olive mill wastewater by Lactobacillus plantarum. Process Biochem 2003;39:59-5.10.1016/S0032-9592(02)00314-X
Search in Google Scholar
Mekki A, Dhouib A, Sayadi S. Polyphenols dynamics and phytotoxicity in a soil amended by olive mill wastewaters. J Environ Manage 2007;84:134-40.10.1016/j.jenvman.2006.05.015
Search in Google Scholar
Cossou R, Blakey N, Cannas P. Influence of co-disposal of municipal solid waste and olive vegetation water on the anaerobic digestion of a sanitary landfill. Water Sci Technol 1993;27:261-71.10.2166/wst.1993.0117
Search in Google Scholar
Aggelis G, Inconomou D, Christou M, Bokas D, Kotzailias S, Christou G, Tsgou V, Papanikolaou S. Phenolic removal in a model olive oil mill wastewater using Pleurotus ostreatus in bioreactor cultures and biological evaluation of the process. Water Res 2003;37:3897-904.10.1016/S0043-1354(03)00313-0
Search in Google Scholar
Peixoto F, Martins F, Amaral C, Gomez-Laranjo J, Almeida J, Palmeira CM. Evaluation of olive oil mill wastewater toxicity on the mitochondrial bioenergetics after treatment with Candida oleophila. Ecotoxicol Environ Safety 2008;70:266-75.10.1016/j.ecoenv.2007.11.003
Search in Google Scholar
Paixão SM, Mendonça E, Picado A, Anselmo AM. Acute toxicity evaluation of olive mill wastewaters: a comparative study of tree aquatic organisms. Environ Toxicol 1999;14:263-9.10.1002/(SICI)1522-7278(199905)14:2<;263::AID-TOX7>3.0.CO;2-D
Search in Google Scholar
Amaral C, Lucas MS, Coutinho J, Crespí AL, do Rosário Anjos M, Pais C. Microbiological and physicochemical characterization of olive mill wastewaters from a continuous olive mill in Northeastern Portugal. Bioresour Technol 2008;99:7215-23.10.1016/j.biortech.2007.12.058
Search in Google Scholar
Ben Sassi A, Ouazzani N, Walker GM, Ibnosouda S, El Mzibri M. Detoxification of olive mill wastewaters by Moroccan yeast isolates. Biodegradation 2008;19:337-46.10.1007/s10532-007-9140-8
Search in Google Scholar
Fadil K, Chahlaoui A, Ouahbi A, Zaid A, Borja R. Aerobic biodegradation and detoxification of wastewaters from the olive oil industry. Int Biodet Biodeg 2003;51:37-41.10.1016/S0964-8305(02)00073-2
Search in Google Scholar
Asses N, Ayed L, Bouallagui H, Sayadi S, Hamdi M. Biodegradation of different molecular-mass polyphenols derived from olive mill wastewater by Geotrichum candidum. Int Biodet Biodeg 2009;63:407-13.10.1016/j.ibiod.2008.11.005
Search in Google Scholar
McNamara CJ, Anastasiou CC, O'Flaherty V, Mitchell R. Bioremediation of olive mill wastewater. Int Biodet Biodeg 2008;61:127-34.10.1016/j.ibiod.2007.11.003
Search in Google Scholar
Tziotzios G, Michailakis S, Vayenas DV. Aerobic biological treatment of olive mill wastewater by olive pulp bacteria. Int Biodet Biodeg 2007;60:209-14.10.1016/j.ibiod.2007.03.003
Search in Google Scholar
Asses N, Ayed L, Bouallagui H, Ben Rejeb I, Gargouri M, Hamdi M. Use of Geotrichum candidum for olive mill wastewater treatment in submerged and static culture. Bioresour Technol 2009;100:2182-8.10.1016/j.biortech.2008.10.048
Search in Google Scholar
Saadi I, Laor Y, Raviv M, Medina S. Land spreading of olive mill wastewater: effects on soil microbial activity and potential phytotoxicity. Chemosphere 2007;66:75-83.10.1016/j.chemosphere.2006.05.019
Search in Google Scholar
Roig A, Cayuela mL, Sánchez-Monedero MA. An overview on olive mill wastes and their valorisation methods. Waste Manage 2006;26:960-9.10.1016/j.wasman.2005.07.024
Search in Google Scholar
El Hajjouji H, Ait Baddi G, Yaacoubi A, Hamdi H, Winterton P, Revel JC, Hafidi M. Optimisation of biodegradation conditions for the treatment of olive mill wastewater. Bioresour Technol 2008;99:5505-10.10.1016/j.biortech.2007.11.005
Search in Google Scholar
Hamdi M. Toxicity and biodegradability of olive mill wastewaters batch anaerobic digestion. Appl Biochem Biotechnol 1992;37:155-62.10.1007/BF02921667
Search in Google Scholar
Borja R, Martin A, Maestro R, Alba J, Fiestas JA. Enhancement of the anaerobic digestion of olive mill wastewater by the removal of phenolic inhibitors. Process Biochem 1992;27:231-7.10.1016/0032-9592(92)80023-V
Search in Google Scholar
Dvoraček L, Glancer M, Ban S. Degradation of phenol in industrial waste waters. Part I: Selection of microorganisms. Prehrambeno-Tehnol Biotehnol Rev 1989;27:147-54.
Search in Google Scholar
Aleksieva Z, Ivanova D, Godjevargova T, Atanasov B. Degradation of some phenol derivatives by Trichosporon cutaneum R57. Process Biochem 2002;37:1215-9.10.1016/S0032-9592(01)00336-3
Search in Google Scholar
Godjevargova T, Ivanova D, Alexieva A, Dimova N. Biodegradation of toxic organic components from industrial phenol production waste waters by free and immobilized Trichosporon cutaneum R57. Process Biochem 2003;38:915-20.10.1016/S0032-9592(02)00170-X
Search in Google Scholar
Chtourou M, Ammar E, Nasri M, Medhioub K. Isolation of a yeast, Trichosporon cutaneum, able to use low molecular weight phenolic compounds: application to olive mill waste water treatment. J Chem Technol Biotechnol 2004;79:869-78.10.1002/jctb.1062
Search in Google Scholar
García García I, Jiménez Peña PR, Bonilla Venceslada JL, Martín Martín A, Martín Santos MA, Ramos Gómez E. Removal of phenol compounds from olive mill wastewater using Phanerochaete chrysosporium, Aspergillus niger, Aspergillus terreus and Geotrichum candidum. Process Biochem 2000;35:751-8.10.1016/S0032-9592(99)00135-1
Search in Google Scholar
Assas N, Ayed L, Marouani L, Hamdi M. Decolorization of fresh and stored-black olive mill wastewaters by Geotrichum candidum. Process Biochem 2002;38:361-5.10.1016/S0032-9592(02)00091-2
Search in Google Scholar
Ayed L, Assas N, Sayadi S, Hamdi M. Involvement of lignin peroxidase in the decolourization of black olive mill wastewaters by Geotrichum candidum. Lett Appl Microbiol 2005;40:7-11.10.1111/j.1472-765X.2004.01626.x15612995
Search in Google Scholar
Gonçalves C, Lopes M, Ferreira JP, Belo I. Biological treatment of olive mill wastewater by non-conventional yeasts. Bioresour Technol 2009;100:3759-63.10.1016/j.biortech.2009.01.00419231162
Search in Google Scholar
Clesceri LS, Greenberg AE, Rhodes Trussell R, editors. Standard Methods for the Examination of Water and Wastewater. 20^th edition. Washington (DC): American Public Health Association, American Water Works Association and Water Pollution Control Federation; 1998.
Search in Google Scholar
Waterman PG, Mole S. Analysis of phenolic plant metabolites. In: Lawton JH, Likens GE, editors. Methods in ecology. Oxford: Blackwell Scientific; 1994. p. 83-5.
Search in Google Scholar

Biodegradation of Olive Mill Wastewater by Trichosporon Cutaneum and Geotrichum Candidum

Abstract

Paradigm

My account