Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Maja Šrut; Anamaria Štambuk; Mirjana Pavlica; Göran Klobučar

doi:10.2478/10004-1254-61-2010-1988

Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Archives of Industrial Hygiene and Toxicology

Volume 61 (2010): Issue 1 (March 2010)

By: Maja Šrut, Anamaria Štambuk, Mirjana Pavlica and Göran Klobučar

Open Access

|Mar 2010

Abstract

Genotoxic effects are often the earliest signs of pollution-related environmental disturbance. In this study, we used the comet assay and micronucleus test to assess DNA damage in the erythrocytes of the European sea bass (Dicentrarchus labrax) exposed to environmental pollution in situ. Fish were collected from a fish farm in the Trogir Bay and their cages placed at an unpolluted reference site Šolta (Nečujam Bay) and a polluted site Vranjic (Kaštela Bay) for four weeks. A group of fish which remained at the fish farm Trogir Bay were used as the second control group. Fish exposed at the Vranjic site showed a significantly higher erythrocyte DNA damage, measured by the comet assay, than either control group. Micronucleus induction showed a similar gradient of DNA damage, but did not reach statistical significance. Our results show that cage exposure of a marine fish D. labrax can be useful in environmental biomonitoring and confirm the comet assay as a suitable tool for detecting pollution-related genotoxicity.

References

Vlahogianni T, Dassenakis M, Scoullos MJ, Valavanidis A. Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in musselsfor assessing heavy metals' pollution in coastal areas from the Saronikos Gulf of Greece.2007;54:1136-71.
Open DOI Search in Google Scholar
Jakšić Ž, Batel R, Bihari N, Mičić M, Zahn RK. Adriatic coast as a microcosm for global genotoxic marine contamination - a long-term field study.2005;50:1314-27.
Open DOI Search in Google Scholar
Knezić S, Margeta J. Integrated management of coastal sewerage systems: the case of Kaštela Bay, Croatia.2002;16:279-305.
Open DOI Search in Google Scholar
Milun V, Barić A, Zvonarić T. Temporal and spatial distribution of chlorinated hydrocarbons in mussels from the Kaštela Bay (Adriatic Sea).2004;13:1237-43.
Search in Google Scholar
Kljaković-Gašpić Z, Odžak N, Ujević I, Zvonarić T, Horvat M, Barić A. Biomonitoring of mercury in polluted coastal area using transplanted mussels.2006;368:199-209.
Open DOI Search in Google Scholar
Russo C, Rocco L, Morescalchi MA, Stingo V. Assessment of environmental stress by the micronucleus test and the Comet assay on the genome of teleost populations from two natural environments.2004;57:168-74.
Open DOI Search in Google Scholar
Ahmad I, Maria VL, Oliveira M, Serafim A, Bebianno MJ, Pacheco M, Santos MA. DNA damage and lipid peroxidation vs. protection responses in the gill ofL. from a contaminated coastal lagoon (Ria de Aveiro, Portugal).2008;406:298-397.
Open DOI Search in Google Scholar
Gustavino B, Scornajenghi KA, Minissi S, Ciccotti E. Micronuclei induced in erythrocytes of(teleostei, pisces) by X-rays and colchicine.2001;494:151-9.
Open DOI Search in Google Scholar
de Lemos CT, Rödel PM, Terra NR, Oliveira NCD'A, Erdtmann B. River water genotoxicity evaluation using micronucleus assay in fish erythrocytes.2007;66:391-401.
Open DOI Search in Google Scholar
Pandrangi R, Petras M, Ralph S, Vrzoc M. Alkaline single cell gell (Comet) assay and genotoxicity monitoring using bullheads and carp.1995;26:345-56.
Open DOI Search in Google Scholar
Devaux A, Flammarion P, Bernardon V, Garric J, Monod G. Monitoring of the chemical pollution of the river Rhône through measurement of DNA damage and cytochrome P4501A induction in Chub ().1998;46:257-62.
Open DOI Search in Google Scholar
Brown JS, Steinert SA. DNA damage and biliary PAH metabolites in flatfish from Southern California Bays and harbors, and the Channel Islands.2004;3:263-74.
Open DOI Search in Google Scholar
de Andrade VM, da Silva J, da Silva FR, Heuser VD, Dias JF, Yoneama ML, de Freitas TRO. Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test.2004;44:459-68.
Open DOI Search in Google Scholar
Whitehead A, Kuivila KM, Orlando JL, Kotelevtsev S, Anderson SL. Genotoxicity in native fish associated with agricultural runoff events.2004;23:2868-77.
Open DOI Search in Google Scholar
Winter M, Day N, Hayes RA, Taylor EW, Butler PJ, Chipman JK. DNA strand breaks and adducts determined in feral and caged chub () exposed to rivers exhibiting variable water quality around Birmingham, UK.2004;552:163-75.
Open DOI Search in Google Scholar
Guilherme S, Vélega M, Pereira ME, Santos MA, Pacheco M. Erythrocytic nuclear abnormalities in wild and caged fish () along an environmental mercury contamination gradient.2008;70:411-21.
Open DOI Search in Google Scholar
Oikari A. Caging techniques for field exposures of fish to chemical contaminants.2006;78:370-81.
Open DOI Search in Google Scholar
Pellacani C, Buschini A, Furlini M, Poli P, Rossi C. A battery ofandtests useful for genotoxic pollutant detection in surface waters.2006;77:1-10.
Open DOI Search in Google Scholar
Frenzilli G, Nigro M, Lyons BP. The Comet assay for the evaluation of genotoxic impact in aquatic environments.2009;681:80-92.
Open DOI Search in Google Scholar
Hartl MGJ, Kilemade M, Sheehan D, Mothersill C, O'Halloran J, O'Brien NM, van Pelt FNAM. Hepatic biomarkers of sediment-associated pollution in juvenile turbot,L.2007;64:191-208.
Open DOI Search in Google Scholar
Akcha F, Leday G, Pfohl-Leszkowicz A. Potential value of the Comet assay and DNA adduct measurement in dab () for assessment ofexposure to genotoxic compounds.2003;534:21-32.
Open DOI Search in Google Scholar
Akcha F, Vincent Hubert F, Pfohl-Leszkowicz A. Measurement of DNA adducts and strand breaks in dab () collected in the field: effects of biotic (age, sex) and abiotic (sampling site and period) factors on the extent of DNA damage.2004;552:197-207.
Open DOI Search in Google Scholar
Lyons BP, Stentiford GD, Bignell J, Goodsir F, Sivyer DB, Devlin MJ, Lowe D, Beesley A, Pascoe CK, Moore MN, Garnacho E. A biological effects monitoring survey of Cardigan Bay using flatfish histopathology, cellular biomarkers and sediment bioassays: Findings of the Prince Madog Prize 2003.2006;62(Suppl 1): S342-6.
Open DOI Search in Google Scholar
Barbee GC, Barich J, Duncan B, Bickham JW, Matson CW, Hintze CJ, Autenrieth RL, Zhou G-D, McDonald TJ, Cizmas L, Norton D, Donnelly KC.biomonitoring of PAH-contaminated sediments using juvenile coho salmon ().2008;71:454-64.
Open DOI Search in Google Scholar
Bony S, Gillet C, Bouchez A, Margoum C, Devaux A. Genotoxic pressure of vineyard pesticides in fish: Field and mesocosm surveys.2008;89:197-203.
Open DOI Search in Google Scholar
Maria VL, Correia AC, Santos MA. Genotoxic and biochemical responses in caged eel (L.) after short-term exposure to harbour waters.2004;29:923-9.
Open DOI Search in Google Scholar
Maria VL, Gravato C, Correia AC, Santos MA. Biotransformation and genotoxicity responses to PAHs in two teleost species.2002;11:609-15.
Search in Google Scholar
Gravato C, Santos MA. β-naphthoflavone liver EROD and erythrocytic nuclear abnormality induction in juvenileL.2002;52:69-74.
Open DOI Search in Google Scholar
Gravato C, Santos MA. Juvenile sea bass liver biotransformation induction and erythrocytic genotoxic responses to resin acids.2002;52:238-47.
Open DOI Search in Google Scholar
Gravato C, Santos MA. Genotoxicity biomarkers' association with B(a)P biotransformation inL.2003;55:352-8.
Open DOI Search in Google Scholar
Teles M, Gravato C, Pacheco M, Santos MA. Juvenile sea bass biotransformation, genotoxic and endocrine responses to β-naphthoflavone, 4-nonylphenol and 17β-estradiol individual and combined exposures.2004;57:147-58.
Open DOI Search in Google Scholar
Teles M, Pacheco M, Santos MA. Biotransformation, stress and genotoxic effects of 17β-estradiol in juvenile sea bass (L.).2006;32:470-7.
Open DOI Search in Google Scholar
Maria VL, Ahmad I, Santos MA. Juvenile sea bass (L.) DNA strand breaks and lipid peroxidation response following 17β-estradiol two mode of exposures.2008;34:23-9.
Open DOI Search in Google Scholar
Gravato C, Santos MA, Magalhães I. JuvenileL. biochemical and genotoxic responses after short-term exposure to β-naphthoflavone and contaminated harbour waters.2000;9:269-74.
Search in Google Scholar
Gravato C, Santos MA. Juvenile sea bass liver biotransformation and erythrocytic genotoxic responses to pulp mill contaminants.2002;53:104-12.
Open DOI Search in Google Scholar
Gravato C, Santos MA.biotransformation and genotoxicity responses after exposure to a secondary treated industrial/urban effluent.2003;55:300-6.
Open DOI Search in Google Scholar
Mohmood I, Maria VL, Oliveira M, Ahmad I, Pacheco M, Santos MA. Seasonal assessment of a contaminated coastal lagoon (Ria de Aveiro, Portugal) using Dicentrarchus labrax L. erythrocytic nuclear abnormalities.2008;17:1924-31.
Search in Google Scholar
Stien X, Percic P, Gnassia-Berelli M, Roméo M, Lafaurie M. Evaluation of biomarkers in caged fishes and mussels to assess the quality of waters in a Bay of the NW Mediterranean Sea.1998;99:339-45.
Open DOI Search in Google Scholar
Klobučar GIV, Štambuk A, Hylland K, Pavlica M. Detection of DNA damage in haemocytes ofin the coastal ecosystems of Kaštela and Trogir Bays, Croatia.2008;405:330-7.
Open DOI Search in Google Scholar
Klobučar GIV, Pavlica M, Erben R, Papeš D. Application of the micronucleus and Comet assays to musselhaemocytes for genotoxicity monitoring of freshwater environments.2003;64:15-23.
Open DOI Search in Google Scholar
Kirsch-Volders M, Sofuni T, Aaderma M, Albertini S, Eastmond D, Fenech M, Ishidate M, Lorge E, Norppa H, Surallés J, von der Hude W, Wakata A. Report from themicronucleus assay working group.2000;35:167-72.
Open DOI Search in Google Scholar
Cotelle S, Férard JF. Comet assay in genetic ecotoxicology: a review.1999;34:246-55.
Open DOI Search in Google Scholar
Odžak N, Zvonarić T, Kljaković-Gašpić Z, Horvat M, Barić A. Biomonitoring of mercury in the Kaštela Bay using transplated mussels.2000;261:61-8.
Open DOI Search in Google Scholar
Ujević I, Odžak N, Barić A. Trace metal accumulation in different grain size fractions of the sediments from semi enclosed bay heavily contaminated by urban and industrial wastewaters.2000;34:3055-61.
Open DOI Search in Google Scholar
Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells.1988;175:184-91.
Open DOI Search in Google Scholar
Kirsch-Volders M, Sofuni T, Aaderma M, Albertini S, Eastmond D, Fenech M, Ishidate M, Kirchner S, Lorge E, Morita T, Norppa H, Surallés J, Vanhauwaert A, Wakata A. Report from themicronucleus assay working group.2003;540:153-63.
Open DOI Search in Google Scholar
Klobučar GIV, Štambuk A, Pavlica M, Sertić-Peroć M, Kutuzović-Hackenberger B, Hylland K. Genotoxicity monitoring of freshwater environments using caged carp ().2010;19:77-84.
Open DOI Search in Google Scholar
Wirzinger G, Weltje L, Gercken J, Sordyl H. Genotoxic damage in field-collected three-spined sticklebacks (L.): a suitable biomonitoring tool?2007;628:19-30.
Search in Google Scholar
Udroiu I. The micronucleus test in piscine erythrocytes.2006;79:201-4.
Open DOI Search in Google Scholar
Sola L, Bressanello S, Rossi AR, Laselli V, Crosetti D, Cataudella S. A karyotype analysis of the genusby different staining techniques.1993;43:329-37.
Open DOI Search in Google Scholar
Chu S, Xi Z, Xu X, Zhang Y, Xu Y. Induction of micronuclei in peripheral erythrocytes ofby polychlorinated biphenyls.1996;57:179-82.
Open DOI Search in Google Scholar
Peichel CL, Nereng KS, Ohgi KA, Cole BLE, Colosimo PF, Buerklet CA, Schluter D, Kingsley DM. The genetic architecture of divergence between threespine stickleback species.2001;414:901-5.
Open DOI Search in Google Scholar
Lee RF, Steinert S. Use of the single cell gel electrophoresis/Comet assay for detecting DNA damage in aquatic (marine and freshwater) animals.2003;544:43-64.
Open DOI Search in Google Scholar
Bombail V, Aw D, Gordon E, Batty J. Application of the Comet and micronucleus assays to butterfish () erythrocytes from the Firth of Forth, Scotland.2001;44:383-92.
Open DOI Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.2478/10004-1254-61-2010-1988 | Journal eISSN: 1848-6312 (formerly 0004-1254) | Journal ISSN: 0004-1254

Journal RSS Feed

Language: English, Croatian

Page range: 29 - 36

Published on: Mar 25, 2010

Published by: Institute for Medical Research and Occupational Health

In partnership with: Paradigm Publishing Services

Keywords:

Adriatic Sea,

comet assay,

fish,

ecogenotoxicology,

marine biomonitoring,

micronucleus test

Related subjects:

Medicine,

Basic medical science,

Basic medical science, other

© 2010 Maja Šrut, Anamaria Štambuk, Mirjana Pavlica, Göran Klobučar, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons License.

Volume 61 (2010): Issue 1 (March 2010)