Determination of 8-hydroxy-2'deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Domijan, Ana-Marija; Peraica, Maja

doi:10.2478/10004-1254-59-2008-1879

Abstract

Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50 nmol L^-1 to 400 nmol L^-1, the calibration curve was linear (r²=0.9995) and the limit of detection was 3 nmol L^-1. Concentrations of 8-oxodG ranged between 3.0 nmol L^-1 and 48.0 nmol L^-1, which is in accordance with values reported for healthy individuals in other studies.

References

Halliwell B. Why and how should we measure oxidative DNA damage in nutritional studies? How far we come? Am J Clin Nutr 2000;72:1082-7.10.1093/ajcn/72.5.1082
Search in Google Scholar
Rebelo IA, Piedade JAP, Oliveira-Brett AM. Development of an HPLC method with electrochemical detection of femtomoles of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2'deoxyguanosine in the presence of uric acid. Talanta 2004;63:323-31.10.1016/j.talanta.2003.10.051
Search in Google Scholar
Wu LL Chiou C-C, Chang P-Y, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta 2004;339:1-9.10.1016/j.cccn.2003.09.010
Search in Google Scholar
Loft S, Poulsen HE. Estimation of oxidative DNA damage in man from urinary excretion of repair products. Acta Biochim Pol 1998;45:133-44.10.18388/abp.1998_4328
Search in Google Scholar
Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga R. Oxidative DNA damage: assessment of the role in carcinogenesis, stherosclerosis, and aquired immunodeficiency syndrome. Free Radic Biol Med 2002;33:192-200.10.1016/S0891-5849(02)00878-X
Search in Google Scholar
Samcova E, Marhol P, Opekar F, Langmaier J. Determination of urinary 8-hydroxy-2-deoxyguanosine in obese patients by HPLC with electrochemical detection. Anal Chim Acta 2004;516:107-10.10.1016/j.aca.2004.04.018
Search in Google Scholar
Xu GW, Yao QH, Weng QF, Su BL, Zhang X, Xiong JH. Study of urinary 8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in diabetic nephropathy patients. J Pharm Biomed Anal 2004;36:101-4.10.1016/j.jpba.2004.04.016
Search in Google Scholar
Spinosa de Martinis B, Pires Bianchi ml. Methodology for urinary 8-hydroxy-2'-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res 2002;46:129-31.10.1016/S1043-6618(02)00080-4
Search in Google Scholar
Germadnik D, Pilger A, Rüdiger HW. Assay for the determination of urinary 8-hydroxy-2'-deoxyguanosine by high-performance liquid chromatography with electrochemical detection. J Chromatogr B 1997;689:399-403.10.1016/S0378-4347(96)00328-3
Search in Google Scholar
Esaka Y, Inagaki S, Goto M. Separation procedures capable of revealing DNA adducts. J Chromatogr B 2003;797:321-9.10.1016/S1570-0232(03)00607-X
Search in Google Scholar

Determination of 8-hydroxy-2'deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Abstract

Paradigm

My account