Structures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024

Evelina Leont’evna Zdorovenko; Alexander Stepanovich Shashkov; Alexandra Alexeevna Kadykova; Artem Nikolaevich Fakhrutdinov; Elena Pavlovna Kiseleva; Danuta Tofilevna Plotnikava; Galina Ivanovna Novik; Yuriy Alexandrovich Knirel

doi:10.24190/ISSN2564-615X/2017/01.07

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Structures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024

The EuroBiotech Journal

Volume 1 (2017): Issue 1 (January 2017)

By: Evelina Leont’evna Zdorovenko, Alexander Stepanovich Shashkov, Alexandra Alexeevna Kadykova, Artem Nikolaevich Fakhrutdinov, Elena Pavlovna Kiseleva, Danuta Tofilevna Plotnikava, Galina Ivanovna Novik and Yuriy Alexandrovich Knirel

Open Access

|Jan 2017

Abstract

Glycopolymers of two types were isolated from the cell wall of Lactococcus lactis BIM B-1024 by stepwise extraction with cold and hot 10% CCl₃CO₂H and separated by anion-exchange gel chromatography. The following structures of the glycopolymers were established by sugar analysis, dephosphorylation with 48% HF, 1D and 2D NMR spectroscopy, and ESI-MS:

β-D-Galp-(1→4)-β-D-Glcp-(1→6)┐

→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp-(1→ PSI

β-D-Glcp-(1→3)┐

→60-α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→5)-Rib-ol-1-P-(O→ PSII

β-D-Glcp-(1→4)┘

Polysaccharides with the same or similar structures to PSI have been found earlier in various Lactobacillus species, whereas, to our knowledge, the structure of PSII is new.

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DOI: https://doi.org/10.24190/ISSN2564-615X/2017/01.07 | Journal eISSN: 2564-615X

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Language: English

Page range: 41 - 45

Submitted on: Jan 27, 2017

Published on: Jan 27, 2017

Published by: European Biotechnology Thematic Network Association

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© 2017 Evelina Leont’evna Zdorovenko, Alexander Stepanovich Shashkov, Alexandra Alexeevna Kadykova, Artem Nikolaevich Fakhrutdinov, Elena Pavlovna Kiseleva, Danuta Tofilevna Plotnikava, Galina Ivanovna Novik, Yuriy Alexandrovich Knirel, published by European Biotechnology Thematic Network Association
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Volume 1 (2017): Issue 1 (January 2017)