Mycosynthesis of Size-Controlled Silver Nanoparticles through Optimization of Process Variables by Response Surface Methodology

Figures & Tables

Runs	Factor A: Substrate concentration (mM)	Factor B: NaCl concentration (%)	Factor C: Wet weight of biomass (g)	Response: Size of AgNPs (nm)
1	1	10	7	33.65
2	3	20	7	127.20
3	2	15	8.5	68.23
4	1	20	10	33.65
5	3	1	10	76.22
6	1	20	7	33.65
7	2	15	8.5	68.20
8	2	15	8.5	68.30
9	2	23.41	8.5	89.37
10	1	10	10	33.65
11	3	20	10	70.48
12	3.68	15	8.5	156.2
13	2	15	11.02	33.65
14	2	15	8.5	33.55
15	2	15	8.5	33.65
16	0.32	15	8.5	126.30
17	2	15	5.98	33.23
18	2	6.59	8.5	33.45
19	3	10	7	127.10
20	2	15	8.5	90.06

Source	Sum of Squares	Df	Mean Square	F-Value	p-value	Prob > F
Model	23241.50	9	2582.39	3.91	0.0224	SignificantSignificant
A – Substrate concentration	7343.55	1	7343.55	11.11	0.0076
B – NaCl concentration	572.28	1	572.28	0.87	0.3740
C – Wet weight of biomass	836.67	1	836.67	1.27	0.2868
AB	3.98	1	3.98	0.006017	0.9397
AC	1447.22	1	1447.22	2.19	0.1697
BC	4.26	1	4.26	0.006451	0.9376
A²	9658.74	1	9658.74	14.62	0.0034	Significant
B²	78.85	1	78.85	0.12	0.7369
C²	2154.83	1	2154.83	3.26	0.1011
Residual	6608.52	10	660.85
Lack of Fit	4113.14	5	822.63	1.65	0.2984	Not significant
Pure Error	2495.38	5	499.08
Core Total	29850.02	19
Std. dev	C.V	R-Squared	Adj R-Squared	Pred R-Squared	Adeq Precision
25.71	37.53	0.7786	0.5794	0.1664	8.801