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Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3 Cover

Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3

Polish Journal of Microbiology
Volume 68 (2019): Issue 1 (March 2019)
By: NOR AFIFAH SUPARDY,  DARAH IBRAHIM,  SHARIFAH RADZIAH MAT NOR and  WAN NORHANA MD NOORDIN  
Open Access
|Mar 2019

Figures & Tables

Fig. 1.

Phylogenetic tree of Pseudoalteromonas sp. IBRL PD4.8 isolated from the surface of C. racemosa.
Phylogenetic tree of Pseudoalteromonas sp. IBRL PD4.8 isolated from the surface of C. racemosa.

Fig. 2.

Agar-overlay assay of ethyl acetate extract of Pseudoalteromonas sp. IBRL PD4.8 against V. alginolyticus FB3.Circle (1) light yellow, Rf 0.06; (2) fluorescent blue, Rf 0.35; (3) fluorescent green, Rf 0.55.
Agar-overlay assay of ethyl acetate extract of Pseudoalteromonas sp. IBRL PD4.8 against V. alginolyticus FB3.Circle (1) light yellow, Rf 0.06; (2) fluorescent blue, Rf 0.35; (3) fluorescent green, Rf 0.55.

Fig. 3.

Antibiofilm activity of Pseudoalteromonas sp. IBRL PD4.8 extract against initial and pre-formed biofilm of V. alginolyticus FB3. Data of inhibition percentages are presented in the table below the figure.
Antibiofilm activity of Pseudoalteromonas sp. IBRL PD4.8 extract against initial and pre-formed biofilm of V. alginolyticus FB3. Data of inhibition percentages are presented in the table below the figure.

Fig. 4.

The light microscopy images of V. alginolyticus FB3 biofilms treated with Pseudoalteromonas sp. IBRL PD4.8 extract.A. Untreated initial biofilm; B. initial biofilm treated with 8.0 mg/ml; C. untreated pre-formed biofilm; D. pre-formed biofilm treated with 8.0 mg/ml; bar = 20 µm.
The light microscopy images of V. alginolyticus FB3 biofilms treated with Pseudoalteromonas sp. IBRL PD4.8 extract.A. Untreated initial biofilm; B. initial biofilm treated with 8.0 mg/ml; C. untreated pre-formed biofilm; D. pre-formed biofilm treated with 8.0 mg/ml; bar = 20 µm.

Fig. 5.

The scanning electron microscopic images of V. alginolyticus FB3 biofilms treated with the extract at a concentration of 8.0 mg/ml.A. untreated initial biofilm (10 000×); B. treated initial biofilm (10 000×); C. untreated pre-formed biofilm (5000×); D. treated pre-formed biofilm (5000×).
The scanning electron microscopic images of V. alginolyticus FB3 biofilms treated with the extract at a concentration of 8.0 mg/ml.A. untreated initial biofilm (10 000×); B. treated initial biofilm (10 000×); C. untreated pre-formed biofilm (5000×); D. treated pre-formed biofilm (5000×).

MIC values for crude extract and fraction F3 against fouling bacteria_

Fouling bacteriaCrude (mg/ml), MICF3 (mg/ml), MIC
Vibrio alginolyticus FB38.008.00
Pseuodoaltermonas sp. FB48.004.00
Alteromonas sp. FB78.008.00
Pseudoalteromonas sp. FB98.008.00
Bacillus sp. FB130.130.50

Characteristics of compounds from LC-MS analysis of sub-fraction F3a_

No.RT (min)CompoundMassm/z [M-H]−FormulaScore (DB)
14.233Sulfate97.96796.9597H2O4S98.79
24.9064,7,10,13-hexadecatetraenoic acid248.1763293.1748C16H24O284.93
35.662Homoveratric acid196.0729241.071C10H12O495.41
46.809Isoacitretin326.1891325.1821C21H26O384.11
56.923Sodium tetradecyl sulfate294.1859293.1779C14H30O4S86.66
67.617D1-2-Hydroxymethylethisteron340.2048339.1978C22H8O385.00

Details of BLAST result, % similarity, and accession numbers of all isolates_

IsolateSequence length (bp)BLAST result% SimilarityAccession No.
PD4.81385Pseudoalteromonas shiyosakiensis99LC131142.1
FB31349Vibrio alginolyticus97KC884661.1
FB41256Pseudoalteromonas sp.96JX0705058.1
FB71439Alteromonas sp.93EF061415.1
FB91398Pseudoalteromonas sp.94JX075059.1
FB131446Bacillus sp.93DQ448746.1
DOI: https://doi.org/10.21307/pjm-2019-003 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 21 - 33
Published on: Mar 27, 2019
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Pseudoalteromonas sp.,
antibiofilm,
biofouling,
liquid chromatography-mass spectroscopy,
scanning electron microscope
Related subjects:
Life sciences,
Microbiology and virology

© 2019 NOR AFIFAH SUPARDY, DARAH IBRAHIM, SHARIFAH RADZIAH MAT NOR, WAN NORHANA MD NOORDIN, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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