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Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere Cover

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

Polish Journal of Microbiology
Volume 67 (2018): Issue 4 (December 2018)
By: DA CHENG HAO,  SI MENG SONG,  YAN CHENG,  ZHI QIANG QIN,  GUANG BO GE,  BAI LIN AN and  PEI GEN XIAO  
Open Access
|Dec 2018

Figures & Tables

Fig. 1.

A. Time course of the laccase production in four fungal strains. B. Biomass production, represented by the mycelium dry weight, of fungal strains. The 70 ml culture media contained 20 g/l glucose, 5 g/l peptone, and 5% fungal inoculant. Effects of carbon sources (C)/nitrogen sources (D) on the laccase production of four fungal strains. The agitation rate and the incubation temperature were 120 rpm and 28°C respectively.
A. Time course of the laccase production in four fungal strains. B. Biomass production, represented by the mycelium dry weight, of fungal strains. The 70 ml culture media contained 20 g/l glucose, 5 g/l peptone, and 5% fungal inoculant. Effects of carbon sources (C)/nitrogen sources (D) on the laccase production of four fungal strains. The agitation rate and the incubation temperature were 120 rpm and 28°C respectively.

Fig. 2.

A. RG19 (20 mg/l) decolorization by dye-reducing fungal consortia. B. COD removal by the fungal consortium or the single strain. The mixture of reactive dyes consists of RG19, reactive brilliant blue K-3R and X-BR (each in 6.67 mg/l). Error bars are standard deviations (n = 3).
A. RG19 (20 mg/l) decolorization by dye-reducing fungal consortia. B. COD removal by the fungal consortium or the single strain. The mixture of reactive dyes consists of RG19, reactive brilliant blue K-3R and X-BR (each in 6.67 mg/l). Error bars are standard deviations (n = 3).

Fig. 3.

A. GO annotation of biological process, cellular component and molecular function.
A. GO annotation of biological process, cellular component and molecular function.

Fig. 3.

B. Distribution of Unigene expression levels.
B. Distribution of Unigene expression levels.

Fig. 3.

C. No. of DEGs in three comparisons. In a pairwise comparison, the former one is the control and the latter the treatment.D. Common and unique DEGs of three comparisons.
C. No. of DEGs in three comparisons. In a pairwise comparison, the former one is the control and the latter the treatment.D. Common and unique DEGs of three comparisons.

Fig. 4.

Hierarchical cluster analysis of DEG union set of ordi vs. green, ordi vs. guai, and guai vs. green. The redder the color, the greater the upregulation of the gene expression; the bluer the color, the greater the downregulation of the gene expression.
Hierarchical cluster analysis of DEG union set of ordi vs. green, ordi vs. guai, and guai vs. green. The redder the color, the greater the upregulation of the gene expression; the bluer the color, the greater the downregulation of the gene expression.

Fig. 5.

Proposed degradation pathway of RG19 in M. verrucaria.
Proposed degradation pathway of RG19 in M. verrucaria.

Enzyme activities of M_ verrucaria strain DJTU-sh7 in decolorizing RG19_

Enzyme (U/ml)RG19 additionNo RG19
IntracellularExtracellularIntracellularExtracellular
LaccaseND26.241 ± 0.760*ND17.108 ± 0.895
Lignin peroxidase0.164 ± 0.006^0.120 ± 0.006^0.152 ± 0.0170.097 ± 0.002
Mn peroxidaseNDNDNDND
Tyrosinase45.000 ± 3.000^38.667 ± 8.386^42.333 ± 6.42933.667 ± 2.309
Aryl alcohol oxidase14.093 ± 0.056*5.900 ± 0.001*5.244 ± 0.0563.572 ± 0.056
Ferric reductase7.845 ± 0.137**11.788 ± 0.182*6.849 ± 0.0689.000 ± 0.182
Azo reductase22.853 ± 0.544^12.696 ± 0.670^23.242 ± 0.15512.204 ± 0.432
NADH-DCIP reductase3.644 ± 1.547^8.926 ± 0.696^2.603 ± 1.6107.550 ± 2.260
DOI: https://doi.org/10.21307/pjm-2018-050 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 417 - 430
Submitted on: Apr 19, 2018
Accepted on: Jul 10, 2018
Published on: Dec 10, 2018
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Myrothecium,
reactive dye decolorization,
laccase,
transcriptome sequencing,
degradation mechanism
Related subjects:
Life sciences,
Microbiology and virology

© 2018 DA CHENG HAO, SI MENG SONG, YAN CHENG, ZHI QIANG QIN, GUANG BO GE, BAI LIN AN, PEI GEN XIAO, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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