Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation Cover

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Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

Polish Journal of Microbiology

Volume 67 (2018): Issue 3 (September 2018)

By: ANNA WĘGRZYN and EWA FELIS

Open Access

|Sep 2018

Figures & Tables

The scheme of a lab-scale constructed wetlands system, from which the plant samples were collected.

Workflow for isolation of cultivable endophytic bacteria from P. arundinacea plants non-exposed and exposed to DCF and SMX mixed pollution (three plants per condition from each column were harvested).

The number of bacteria (CFU/g FW) obtained from the surface of the sterilized reed canary grass root samples. Light bars – the number of bacterial endophytes grown on nutrient agar. Grey bars – the number of bacterial endophytes grown on nutrient medium enriched with DCF and SMX. N = 6. Significant differences have been marked using * (p < 0.05; t-test). Time regime of wastewater supply in brackets.

The removal of 2 mg/l DCF by MG7 strain in the adsorption control, without additional carbon source and with phenol at a concentration of 100 mg/l as the additional carbon source.

The removal of 2 mg/l SMX by MG7 strain in the adsorption control, without additional carbon source and with phenol at a concentration of 100 mg/l as the additional carbon source.

Changes in biomass of MG7 strain in the cultures that contained DCF, monitored as optical density at 600 nm, without additional carbon source and with phenol at a concentration of 100 mg/l as additional carbon source.

Changes in biomass of MG7 strain in the cultures that contained SMX, monitored as optical density at 600 nm, without additional carbon source and with phenol at a concentration of 100 mg/l as additional carbon source.

DGGE pattern of 16S rRNA gene fragments of 180 bp amplified from DNA obtained from the surface-sterilized root tissues of P. arundinacea.A1-3, C1-3 – the root samples non-exposed to DCF and SMX, B1-3, D1-3 – the root samples exposed to DCF and SMX. The frequency of wastewater supply: A1-3 and B1-3 – 0.35 l four times a day; C1-3 and D1-3 1.4 l – once a day. Description of the bands in the manuscript text.

Shannon biodiversity index based on DGGE profiles.A1-3, C1-3 – the root samples non-exposed on DCF and SMX; B1-3, D1-3 – the root samples exposed on DCF and SMX. The frequency of wastewater supply: A1-3 and B1-3 – 0.35 l four times a day; C1-3 and D1-3 – 1.4 l once a day.

Identification of bacterial endophytes, based on the 16S rRNA gene sequence_

Isolate	Clostest match	Similarity, %	Sequence ID
MG2	Variovorax boronicumulans	96	NR_114214.1
MG5	Bacillus wiedmannii	97	NR_152692.1
MG7	Microbacterium flavescens	98	NR_029350.1
MG11	Agrobacterium tumefaciens	97	NR_041396.1

DOI: https://doi.org/10.21307/pjm-2018-039 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 321 - 331

Submitted on: Oct 30, 2017

Accepted on: May 20, 2018

Published on: Sep 4, 2018

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

endophytic bacteria,

constructed wetlands,

sulfamethoxazole,

Related subjects:

Microbiology and virology

© 2018 ANNA WĘGRZYN, EWA FELIS, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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