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The Effect of Silver Nanoparticles on Listeria monocytogenes PCM2191 Peptidoglycan Metabolism and Cell Permeability Cover

The Effect of Silver Nanoparticles on Listeria monocytogenes PCM2191 Peptidoglycan Metabolism and Cell Permeability

Polish Journal of Microbiology
Volume 67 (2018): Issue 3 (September 2018)
By: KATARZYNA MARKOWSKA,  ANNA MARIA GRUDNIAK,  BARBARA MILCZAREK and  KRYSTYNA IZABELLA WOLSKA  
Open Access
|Sep 2018

Figures & Tables

Fig. 1.

Effect of AgNPs on L. monocytogenes growth (A) and survival (B).Solid lines – control; dashed lines – 0.25 MIC AgNPs; dotted lines – 0.5 MIC AgNPs. Mean ± SD values of triplicate cultures were shown.
Effect of AgNPs on L. monocytogenes growth (A) and survival (B).Solid lines – control; dashed lines – 0.25 MIC AgNPs; dotted lines – 0.5 MIC AgNPs. Mean ± SD values of triplicate cultures were shown.

Fig. 2.

Influence of AgNPs on lysozyme- or Triton X-100-induced autolysis/lysis of L. monocytogenes.White bar – control, buffer only; dark grey bar – lysozyme or Triton X-100; light grey bar – lysozyme or Triton X-100 + 0.25 MIC AgNPs; black bar – lysozyme or Triton X-100 + 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant differences (p < 0.05) were marked with asterisks.
Influence of AgNPs on lysozyme- or Triton X-100-induced autolysis/lysis of L. monocytogenes.White bar – control, buffer only; dark grey bar – lysozyme or Triton X-100; light grey bar – lysozyme or Triton X-100 + 0.25 MIC AgNPs; black bar – lysozyme or Triton X-100 + 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant differences (p < 0.05) were marked with asterisks.

Fig. 3.

Autolysis of isolated L. monocytogenes peptidoglycan in the presence of AgNPs.Absorbance A600 at time 0 was considered as 100%. Solid line – control; dashed line – 0.25 MIC AgNPs; dotted line – 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) was marked with an asterisk.
Autolysis of isolated L. monocytogenes peptidoglycan in the presence of AgNPs.Absorbance A600 at time 0 was considered as 100%. Solid line – control; dashed line – 0.25 MIC AgNPs; dotted line – 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) was marked with an asterisk.

Fig. 4.

Effect of AgNPs influence on the efflux of DNA and proteins from L. monocytogenes.White bars – control, no AgNPs added; dark grey bars – 0.25 MIC AgNPs; light grey bars – 0.5 MIC AgNPs; black bars – 100 μg/ml lysozyme (positive control). The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) were marked with asterisks.
Effect of AgNPs influence on the efflux of DNA and proteins from L. monocytogenes.White bars – control, no AgNPs added; dark grey bars – 0.25 MIC AgNPs; light grey bars – 0.5 MIC AgNPs; black bars – 100 μg/ml lysozyme (positive control). The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) were marked with asterisks.

Increase in cF efflux from L_ monocytogenes caused by AgNPs_

AgNPs concentration% efflux of cF at time (min)
010
0 (negative control)72 ± 279 ± 1
0.25 MIC73 ± 284 ± 2
0.5 MIC74 ± 293* ± 2
DOI: https://doi.org/10.21307/pjm-2018-037 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 315 - 320
Submitted on: Jan 11, 2018
Accepted on: May 10, 2018
Published on: Sep 4, 2018
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
autolysis,
peptidoglycan,
permeability,
silver nanoparticles
Related subjects:
Life sciences,
Microbiology and virology

© 2018 KATARZYNA MARKOWSKA, ANNA MARIA GRUDNIAK, BARBARA MILCZAREK, KRYSTYNA IZABELLA WOLSKA, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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