Table I
Detection of pathogenic Yersinia enterocolitica in pig tonsils with RT-PCR.
| Number of samples | Source | Ct ranges in HEX channel for positive results | Ct ranges in HEX channel for negative results |
|---|---|---|---|
| Positive control | Control DNA | 28.89 | – |
| 17 | Pig tonsils | 25.67–36.17 | – |
| 83 | Pig tonsils | – | 37.23–37.63 |
| Negative control | PCR water | – | 37.34 |
Table II
Assessment of biotypes of Yersinia enterocolitica isolates according to Wauters et al. scheme (1987).
| Strains | Biochemical tests | Biotype | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Lipase (Tween-esterase) | Esculin/salicin 24 h | Indole | Xylose | Trehalose/NO3 | Pyrazinamidase | β-D-Glucosidase | Voges-Proskauer | Proline peptidase | ||
| 2006RAT | + | + | + | + | + | + | + | + | V1 | 1A |
| SDWL-003 | + | + | + | + | + | + | + | + | V1 | 1A |
| Ye92010 | + | – | + | + | + | – | – | + | – | 1B |
| NX-SA98-837 | – | – | – | + | + | – | – | + | – | 3 |
Table III
The adhesion and invasion properties of Yersinia enterocolitica biotype 1A containing the ail gene in comparison to biotype 3.
| Isolate | Biotype | A number of bacterial cells adhering to Hep-2 cells | A number of bacterial cells surviving within Hep-2 cells |
|---|---|---|---|
| 2006RAT | 1A | 5 000 ± 10T | 10 000 ± 12 |
| SDWL-003 | 1A | 1 000 ± 10 | 500 ± 7 |
| Ye92010 | 1B | 750 000 ± 23 | 4 500 ± 10 |
| NX-SA98-837 | 3 | 2 100 000 ± 36 | 32 000 ± 16 |