Transfusion support for a woman with RHD*09.01.02 and the novel RHD*01W.161 allele in trans

Figures & Tables

	RHD alleles
Test	Allele 1	Allele 2
RBC-FluoGene D weak/variant	RHD01W.14*	Possible RHD01W.14* or RHD01* or RHD01N.01*
wRHD BeadChip	RHDDAR*	Possible RHD01*
Nucleotide sequencing
Allele recognized	Known	Novel
Nucleotide sequence (GenBank)	KF712273	MT980847
Amino acid change(s)	T201R, F223V, I342T	I204K
Serologic weak D phenotype¹⁵	DAR1.2 (weak D 4.2.2)¹⁸	Weak D type 161
ISBT terminology¹⁶	RHD09.01.02*¹⁸	RHD01W.161*^†

Test	Result
ABO group	AB (mixed-field agglutination for A)*
RhD phenotype	Serologic weak D phenotype^†
RhCE phenotype	C+E–c+e+
Antibody detection test	Negative
Direct antiglobulin test	Negative

			D variant and serologic reaction strength*
			Study sample	Control samples
Monoclonal anti-D^†				Weak D type
Clone	Isotype	epD	Patient CDe/cDe	19^‡ CDe/cde	1 CDe/cde	4.0^§ cDe/cde	4.1 cDe/cde	4.2.2^¶ cDe/cde	45 CDe/cde	64 cDe/cde	88 cDE/cde	D+ cDe/cde	D– cde/cde
LHM76/58	IgG_1λ	8.1	4+	+	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM76/59	IgG₁	15.1	4+	+	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM174/102	IgG_3κ	1.2	2+	+	0	0	0	0	4+	2+	1+	4+	0
LHM50/2B	IgG_1λ	6.3	4+	NT	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM169/81	IgG_3κ	1.1	4+	+	4+	4+	4+	2+ to 3+	4+	4+	4+	4+	0
ESD1	IgG_1κ	4.1	4+	NT	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM76/55	IgG_1κ	3.1	4+	+	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM77/64	IgG_1κ	9.1	4+	NT	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM70/45	IgG_1λ	1.2	3+	+	3+	3+	3+	0 to 2+	3+	3+	3+	4+	0
LHM59/19	IgG_3κ	8.1	4+	NT	3+	3+	3+	0 to 2+	4+	3+	3+	4+	0
LHM169/80	IgG_3λ	6.3	4+	NT	4+	4+	4+	3+ to 4+	4+	4+	4+	4+	0
LHM57/17	IgG_1λ	6.3	0	NT	0	0	0	0 to 2+	NT	0	0	1+	0
LDM1	IgM	6.5	3+	NT	0	4+	3+	0 to 2+	4+	0	2+	4+	0