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Effect of cryopreservation on a rare McLeod donor red blood cell concentrate

Immunohematology
Volume 37 (2021): Issue 2 (June 2021)
By:
Open Access
|Jun 2021

Figures & Tables

Fig. 1.

Red blood cell (RBC) deformability represented by two index parameters: EIMax, the maximum elongation of RBCs under sheer stress, and KEI, a measure of rigidity, represented by the sheer stress required to reach half of the RBC maximum elongation. Data shown as mean ± 1 standard deviation for the control group (n = 3).
Red blood cell (RBC) deformability represented by two index parameters: EIMax, the maximum elongation of RBCs under sheer stress, and KEI, a measure of rigidity, represented by the sheer stress required to reach half of the RBC maximum elongation. Data shown as mean ± 1 standard deviation for the control group (n = 3).

Fig. 2.

(A) Morphology index of red blood cells (RBCs) stored for up to 14 days post-deglycerolization at 1–6°C. Data shown as mean ± 1 standard deviation for the control group (n = 3). (B) Microscopic images of RBC morphology at 1 and 14 days of hypothermic storage post-deglycerolization from thin smear slide preparations. The control images are from a representative sample taken from preparations of 1 RBC unit from the control group. The control unit images contain RBCs undergoing various states of crenation due to the hypothermic storage lesion, which should not be confused with pathologic acanthocytosis. RBCs from both the control and McLeod units will naturally undergo crenation, which may obscure or artificially increase the degree of acanthocytosis in the McLeod sample.
(A) Morphology index of red blood cells (RBCs) stored for up to 14 days post-deglycerolization at 1–6°C. Data shown as mean ± 1 standard deviation for the control group (n = 3). (B) Microscopic images of RBC morphology at 1 and 14 days of hypothermic storage post-deglycerolization from thin smear slide preparations. The control images are from a representative sample taken from preparations of 1 RBC unit from the control group. The control unit images contain RBCs undergoing various states of crenation due to the hypothermic storage lesion, which should not be confused with pathologic acanthocytosis. RBCs from both the control and McLeod units will naturally undergo crenation, which may obscure or artificially increase the degree of acanthocytosis in the McLeod sample.

In vitro parameters for hypothermically stored RBC units post-deglycerolization using the ACP 215 after manual removal of excess glycerol before processing

Length of hypothermic storage post-deglycerolization
McLeod (n = 1)Control group (n = 3)
Parameter1 day7 days14 days1 day7 days14 days
RBC hemolysis (%) Mean (SD)0.230.48* 0.550.19 (0.02)0.31 (0.02)0.52 (0.10)
ATP (µmol/g Hgb) Mean (SD)4.196* 2.1501.4323.735 (0.223)2.617 (0.512)1.816 (0.349)
MCF (g/L NaCl) † Mean (SD)6.1045.9865.9185.541 (0.688)5.504 (0.656)5.473 (0.653)
Supernatant K+ (µmol/L) Mean (SD)5.2* 15.020.63.7 (0.5)13.2 (1.1)18.8 (1.6)
MCV (fL) Mean (SD)85.1* 84.986.3104.1 (9.3)98.4 (8.7)98.4 (8.4)
DOI: https://doi.org/10.21307/immunohematology-2021-012 | Journal eISSN: 1930-3955 | Journal ISSN: 0894-203X
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Language: English
Page range: 78 - 83
Published on: Jun 25, 2021
Published by: American National Red Cross
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
erythrocyte,
cryopreservation,
McLeod phenotype,
deglycerolization,
genotyping,
acanthocyte
Related subjects:
Medicine,
Clinical medicine,
Laboratory medicine

© 2021 T.R. Turner, G. Clarke, G.A. Denomme, R. Skeate, J.P. Acker, published by American National Red Cross
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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