Glycine-EDTA treatment to prepare Ko red blood cells: preservation of high-frequency antigens

Reactivity of high-incidence antigens after glycine-EDTA treatment of red blood cells (RBCs) to prepare artificial K_o RBCs was investigated. The treatment had little or no effect on Yt^a, JMH, Yk^a, Kn^a, and McC^a. Hy antigen-positive-treated RBCs reacted less well with anti-Hy when compared to nontreated cells. Glycine-EDTA treatment to prepare K_o RBCs offers an advantage over AET treatment because it preserves some high-frequency antigens that AET treatment does not.