Characterization of human anti-hrB-like monoclonal antibody

A.P. Blancher; M.E. Reid; S.J. Alié-Daram; J-M.H. Dugoujon; F.L. Roubinet

doi:10.21307/immunohematology-2019-762

Characterization of human anti-hrB-like monoclonal antibody

Immunohematology

Volume 12 (1996): Issue 3 (September 1996)

By: A.P. Blancher, M.E. Reid, S.J. Alié-Daram, J-M.H. Dugoujon and F.L. Roubinet

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Abstract

To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R₂R₂, R_zR_z, Dc–, D––, Rh_null, and e+ hr^B–. The supernatant is useful as a reagent to screen for hr^B– blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.

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Articles in this issue

DOI: https://doi.org/10.21307/immunohematology-2019-762 | Journal eISSN: 1930-3955 | Journal ISSN: 0894-203X

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Language: English

Page range: 119 - 122

Published on: Nov 16, 2020

Published by: American National Red Cross

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Related subjects:

Medicine,

Clinical medicine,

Laboratory medicine

© 2020 A.P. Blancher, M.E. Reid, S.J. Alié-Daram, J-M.H. Dugoujon, F.L. Roubinet, published by American National Red Cross
This work is licensed under the Creative Commons License.

Volume 12 (1996): Issue 3 (September 1996)