Abstract
To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RzRz, Dc–, D––, Rhnull, and e+ hrB–. The supernatant is useful as a reagent to screen for hrB– blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.