Serologic and molecular investigations of a chimera

Mifsud, Nicole A.; Haddad, Albert P.; EHart, Cathie; Holdsworth, Rhonda; Condon, Jennifer A.; Swain, Michael; Sparrow, Rosemary L

doi:10.21307/immunohematology-2019-626

Abstract

A chimeric individual possesses two or more genetically distinct ceil populations. Although the chimerism may not be evident in all gene systems, various loci display greater numbers of alleles than genetically “normal” individ uals. The proposit’a was referred for further laboratory investigation due to a mixed-field ABO blood group reaction following routine antenatal testing. Various molecular (HLA class II, ABO genotyping, and 10 short tandem repeat [STR] microsatellites) and serologic (HLA class I and red cell blood groups) typing techniques were employed to investigate a number of polymorphic loci located on different chromosomes. Chimerism was identified in 8 out of the 1.4 chromosomes tested: chromosome 1 (Duffy), 6 (HLA class I and Iï), 9 (ABO), 11 (HUMTH01), 12 (HUMPLA2A1), 15 (HUMFES/FPS). 18 (Kidd) and 21 (D21S11). The proposita was determined to be a probable dispermic chimera, based on the results of the serology and molecular studies.

References

Tippett P. Blood group chimeras. Vox Sang 1983;44:333-9.10.1159/000465332
Search in Google Scholar Back to article
Bird GWG. Human blood group mosaics and chimeras. Biotest Bull 1985;2:215-22.
Search in Google Scholar Back to article
Daniels, G. Chimeras: human blood groups. 1st ed. Oxford, England: Blackwell .Science, 1995:685-92.
Search in Google Scholar Back to article
Bromilow IM, Duguid J. Blood group chimerism: a possible further example. Med Lab Sci 1991; 48: 212-6.
Search in Google Scholar Back to article
Green REB, Ford DS, Condon JA, Lowe VA. Basic blood grouping techniques and procedures. 2nd ed. Published by the Victorian Immunohaematology Discussion Group, Melbourne, Victoria, 1992.
Search in Google Scholar Back to article
Beattie K, Mallory D. Immunohematology methods and procedures. 1st ed.American Red Cross, 1993.
Search in Google Scholar Back to article
Peake IR, Bowen DJ,Bignell Ę et al. Family studies and prenatal diagnosis in severe von Willebrand disease by polymerase chain reaction amplification of a variable number tandem repeat region of the von Willebrand factor gene. Blood 1990;76:555-61.10.1182/blood.V76.3.555.555
Search in Google Scholar Back to article
Kimpton C,WaltonA, Gill P.A further tetranucleotide repeat polymorphism in the vWF gene. Hum Mol Genet I992;l:287.10.1093/hmg/1.4.2871303199
Search in Google Scholar Back to article
Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R. Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 1994;55:175-9-
Search in Google Scholar Back to article
Urquhart A, Kimpton CP, Downes TJ, Gill P Variation in short tandem repeat sequences—a survey of twelve microsatellite loci for use as forensic identification markers.Intĵ Leg Med 1994;107:13-20.10.1007/BF012472687999641
Search in Google Scholar Back to article
Mifsud NA, Haddad AP, Condon JA, Sparrow RL ABO genotyping—-identification of O¹,0¹’, and O² alleles using the polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) technique. Immunohematology I996;4:149-53-
Search in Google Scholar Back to article
Terasaki PI. Histocompatibility testing. Los Angeles, CA: UCLA tissue typing laboratory, 1980.
Search in Google Scholar Back to article
Bignon JD, Fernandez-Vina MA. Protocols of the twelfth international histocompatibility workshop for typing of HLA class II alleles by DNA amplification of the polymerase chain reaction (PCR) and hybridization with sequence specific oligonucleotide probes (SSOP). In: Proceedings of the twelfth international histocompatibility workshop and conference. Paris, France: EDK Medical and Scientific International Publisher, 1997.
Search in Google Scholar Back to article
Watt JL, Stephen GS. Lymphocyte culture for chromosome analysis. In: Rooney DE, Czepulkowski BH, eds. Human cytogenetics. Oxford, England: 1978-
Search in Google Scholar Back to article
Chandanayingyong-D, Kunmartini S, et al. HLA in Thais, Thai-Chinese, Vietnamese, Javanese and Timorese. In:Tsuji K,Aizawa M, Sasazuki T, eds. HLA 1991: Proceedings of the eleventh international histocompatibility workshop and conference. Oxford, England:Oxford University Press, 1992:681.
Search in Google Scholar Back to article

Serologic and molecular investigations of a chimera

Abstract

Paradigm

My account