Clinically significant autoimmune hemolytic anemia with a negative direct antiglobulin test by routine tube test and positive by column agglutination method

M. Lai; C. Rumi; G. D’Onofrio; M.T. Voso; G. Leone

doi:10.21307/immunohematology-2019-526

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Clinically significant autoimmune hemolytic anemia with a negative direct antiglobulin test by routine tube test and positive by column agglutination method

Immunohematology

Volume 18 (2002): Issue 4 (December 2002)

By: M. Lai, C. Rumi, G. D’Onofrio, M.T. Voso and G. Leone

Paid access

|Oct 2020

Abstract

In two to five percent of cases of autoimmune hemolytic anemia (AIHA), the patient’s RBCs are negative in the direct antiglobulin test (DAT). We describe a patient with AIHA with a negative DAT when tested by the traditional tube test but with a strongly positive one when tested by column agglutination. When the DAT was repeated by tube test using 4°C saline washes, it became positive. This phenomenon has been observed when low-affinity antibodies are involved in AIHA. The patient’s history and serologic findings are detailed in this report. Immunohematology 2002;18:109–113.

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Articles in this issue

DOI: https://doi.org/10.21307/immunohematology-2019-526 | Journal eISSN: 1930-3955 | Journal ISSN: 0894-203X

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Language: English

Page range: 109 - 113

Published on: Oct 14, 2020

Published by: American National Red Cross

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

direct antiglobulin test,

column agglutination method,

autoimmune hemolytic anemia,

low-affinity antibodies

Related subjects:

Medicine,

Clinical medicine,

Laboratory medicine

© 2020 M. Lai, C. Rumi, G. D’Onofrio, M.T. Voso, G. Leone, published by American National Red Cross
This work is licensed under the Creative Commons License.

Volume 18 (2002): Issue 4 (December 2002)