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Red cell antibody identification by solid phase red cell adherence utilizing dried RBC monolayers Cover

Red cell antibody identification by solid phase red cell adherence utilizing dried RBC monolayers

Paid access
|Dec 2020

Abstract

Recent technological advances in the immobilization and drying of red cell monolayers for use in solid phase red cell adherence (SPRCA) assays have resulted in the development of reagent red cells for antibody screening and identification that are stable at room temperature. Panels consisting of twelve different RBC samples dried onto individual microplate wells were evaluated with 176 samples whose antibody specificities had previously been determined by conventional hemagglutination techniques. Identification tests performed with dried SPRCA panels proved to be more sensitive and less time consuming than hemagglutination tests. The red cell antigens of dried membranes were shown to be stable and reactive following storage for 120 days at room temperature.

DOI: https://doi.org/10.21307/immunohematology-2019-1032 | Journal eISSN: 1930-3955 | Journal ISSN: 0894-203X
Language: English
Page range: 12 - 17
Published on: Dec 20, 2020
Published by: American National Red Cross
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2020 D.L. Stone, R.A. Eatz, S.D. Rolih, S.J. Farlow, G.S. Hudson, L.T. Sinor, published by American National Red Cross
This work is licensed under the Creative Commons License.