Typing of normal and variant red cells with ABO, Rh, and Kell typing reagents using a gel typing system

Tills, D.; Bushrod, J.; Ward, D.J.; Josef, D.

doi:10.21307/immunohematology-2019-1023

Abstract

In 1989 Lapierre et al. described a novel method of detecting agglutination reactions by the use of a Sephadex (DiaMed ID Typing System) gel held in a microtube. This report examines the use of gels containing ABO, Rh, and Kell system specific antibodies. The anti-A and -B were monoclonal reagents; anti-A,B, and those for the Rh and Kell systems were polyclonal. Five hundred and fifty-one tests performed for the ABO system detected all but the most weakly reacting variants, a detection rate superior to most commercially available reagents. Five hundred and thirty samples were typed for Rh antigens. One hundred and twenty-seven of these were of various D category III through VII types (D^cats) and 154 were D^Us. The gel system detected all but seven D^VI variants and seven D^Us. The seven D^VI variants, from individuals with no anti-D in their sera, gave reactions identical to the seven D^Us when tested against a panel of over 50 monoclonal IgG and IgM anti-Ds. The 554 samples tested for the K1 antigen gave correct results. Immunohematology 1991;7:94–97.

References

1. Landsteiner K. Zur kenntnis der antifermentativen, lytischen und agglutinerenden wirkungen des blutserums und der lymphe. Zbl Bakt 1900;27:357–62.
Search in Google Scholar Back to article
2. Landsteiner K. Uber agglutinationserecheinungen normalen menscblichen blutes. Wein Klin Wechr 1901; 14: 1132–4.
Search in Google Scholar Back to article
3. Lapierre Y, Rigal J, Adam A, Joseph D, Meyer F, Greber S, Drot C. The gel test: a new way to detect red cell antigen-antibody reactions. Transfusion 1989;30: 109–13.10.1046/j.1537-2995.1990.30290162894.x
Search in Google Scholar Back to article
4. Tills D, Bushrod J. Evaluation of anti-A monoclonal antibodies. In: First International Workshop on Monoclonal Antibodies against Human Red Blood Cells and Related Antigens. Part I. ABH and other glyconjugates. Revue Française de Transfusion et Immuno-Haematologie 1987;30:397–403.10.1016/S0338-4535(87)80097-1
Search in Google Scholar Back to article
5. Tills D, Bushrod J. Anti-A, B, and A,B monoclonals. In: Proceedings of the Second International Workshop and Symposium on Monoclonal Antibodies against Human Red Blood Cells and Related Antigens. Eds. Chester MA, Johnson U, Lundblad A, Low B, Messeter L, Sameleson B. 1990;64.
Search in Google Scholar Back to article
6. Tills D, Bushrod J, Hoppe PA. Anti-D monoclonals. In: Proceedings of the Second International Workshop and Symposium on Monoclonal Antibodies against Human Red Blood Cells and Related Antigens. Eds. Chester MA, Johnson U, Lundblad A, Low B, Messeter L, Sameleson B. 1990;174.
Search in Google Scholar Back to article
7. Tills D, Bushrod J. Anti-E and anti-e monoclonals. In: Proceedings of the Second International Workshop and Symposium on Monoclonal Antibodies against Human Red Blood Cells and Related Antigens. Eds. Chester MA, Johnson U, Lundblad A, Low B, Messeter L, Sameleson B. 1990;194.
Search in Google Scholar Back to article
8. Tills D, Bushrod J, Ward DJ, McGowan A. Variation of the Rhp) antigen revealed by monoclonal antibodies. Paper presented at the Food and Drug Administration Symposium on Reagents for the 1990's. Blood Bank Reagents Standards Workshop, November 7-9, 1990, Washington, DC.
Search in Google Scholar Back to article

Typing of normal and variant red cells with ABO, Rh, and Kell typing reagents using a gel typing system

Abstract

Paradigm

My account