Understanding the decay of Proteins: A Study of Time dependent Microwave Response of pM concentration of Insulin in Buffer Solution

Ritika Verma; K. S. Daya

doi:10.21307/ijssis-2019-038

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Understanding the decay of Proteins: A Study of Time dependent Microwave Response of pM concentration of Insulin in Buffer Solution

International Journal on Smart Sensing and Intelligent Systems

Volume 7 (2014): Issue 5 (January 2014)

By: Ritika Verma and K. S. Daya

Open Access

|Feb 2020

Abstract

Bio-molecule when isolated from its natural ecological condition is subjected to rapid decay. This decay leads to change in polarization of the molecule and the permittivity. This study presents an experimental analysis of the decay pattern of pM concentration of insulin using whispering gallery mode (WGM) dielectric resonator method. Analysis is carried out by comparing the permittivity, relaxation time and time delay for three days. It is observed that different pM concentrations of insulin solutions start to decay after 24 hours at 5⁰C.

References

Dmitry V. Matyushov and Alexander Y. Morozov, “Electrostatics of the protein-water interface and the dynamical transition in proteins”, PHYSICAL REVIEW E, Vol. 84, pp-011908, 2011.
Search in Google Scholar Back to article
Kyritsis, A. Panagopoulou, P. Pissis, R. S. i. Serra, J. L. Gómez Ribelles, and N. Shinyashiki, ”Water and Protein Dynamics in Protein-Water Mixtures over Wide Range of Composition”, IEEE Transactions on Dielectrics and Electrical
Search in Google Scholar Back to article
O.A. Gorobchenko, O.T. Nikolov, S.V. Gatash, “Conformation transitions of blood proteins under influence of physical factors on microwave dielectric method”, Journal of Quantitative Spectroscopy & Radiative Transfer, vol. 102, pp 18–24, 2006.
Search in Google Scholar Back to article
Nilashis Nandi and Biman Bagchi, “Dielectric Relaxation of Biological Water”, J. Phys. Chem. B,Vol. 101, pp. 10954-10961, 1997. 47452-2, Kluwer Academic Publishers, New York, 2002.
Search in Google Scholar Back to article
Satosi Iwamoto and Hitoshi Kumagai, “Analysis of the Dielectric Relaxation of a Gelatin Solution”, Biosci. Biochem., Vol. 62 (7), 1381-1387,1998.
Search in Google Scholar Back to article
“Formulation, Characterization, and Stability of Protein Drugs: Case Histories”, edited by Rodney Pearlman and Y. John Wang, eBook ISBN: 0-306-Insulation, Vol. 19, No. 4, pp-1239-1246, August 2012.
Search in Google Scholar Back to article
Laogunt A A, Sheppard R J and Grant E H, “Dielectric properties of insulin in solution”, Phys. Med. Biol. 29, 519 (1984).
Search in Google Scholar Back to article
Wang J, Jian-Wei Mo, Erdem A, “Single-Use Thick-Film Electrochemical Sensor for Insulin” Electroanal. 14, 1365 (2002).
Search in Google Scholar Back to article
Snider R M, Ciobanu M, Rue A E, and Cliffel D E, “A multiwalled carbon nanotube/dihydropyran composite film electrode for insulin detection in a microphysiometer chamber”, Anal. Chim. Acta 609, 44 (2008).
Search in Google Scholar Back to article
Salimi A, Noorbakhasha A, Sharifia E, Emnani A, “Highly sensitive sensor for picomolar detection of insulin at physiological pH, using GC electrode modified with guanine and electrodeposited nickel oxide nanoparticles”, Biosens. Bioelectron. 24, 792 (2008).
Search in Google Scholar Back to article
Shaforost E N, N.Klein, Vitusevich S A, Barannik A A, and Cherpak N T, “High sensitivity microwave characterization of organic molecule solutions of nanoliter volume”, Appl. Phys. Lett. 94, 112901 (2009).
Search in Google Scholar Back to article
Shaforost E N, Klein N, Vitusevich S A, Offenhäusser A, and Barannik A A, “Nanoliter liquid characterization by open whispering-gallery mode dielectric resonators at millimeter wave frequencies”, J. Appl. Phys. 104, 074111 (2008).
Search in Google Scholar Back to article
Sheen J, “Amendment of cavity perturbation technique for loss tangent measurement at microwave frequencies”, J. Appl. Phys. 102, 014102 (2007).
Search in Google Scholar Back to article
Udo Kaatze, “Complex Permittivity of Water as a Function of Frequency and Temperature”, J. Chem. Eng. Data, Vol. 34, pp. 371 -374, 1989.
Search in Google Scholar Back to article
Tom Moss and Benoît Leblanc, “DNA-Protein Interactions: Principles and Protocols”, 3^rd Edition. Humana Press, 2009DOI: 10.1007/978-1-60327015-1.
Search in Google Scholar Back to article
Avram Hershko and Gordon M. Tomkins, “Studies on the Degradation of Tyrosine Aminotransferase in Hepatoma Cells in Culture”, The Journal of Biological Chemistry, Vol. 246. No. 3, Issue of February 10, pp. 710-714, 1971.
Search in Google Scholar Back to article
Rolf D. Fussganger,* C. Ronald Kahn, Jesse Roth, And Pierre De Meyts, “Binding and Degradation of Insulin by Human Peripheral Granulocytes”, The Journal of Biological Chemistry,Vol. 251, No. 9. Issue of May 10,pp.2761~2769,1976.
Search in Google Scholar Back to article
Inhibition by Bacitracino f Rat Adipocyte Plasma Membrane Degradation of 1251-InsuliIns Associated with an Increase in Plasma Membrane Bound Insulin and a Potentiation of Glucose Oxidation by Adipocytes”, The Journal of Biological Chemistry, Vol. 257, No. 19. Issue of October 10, pp. 11563-11570, 1982.
Search in Google Scholar Back to article
[19]Basey-Fisher T H, Hanham S M, Andresen H, Maier S A, Stevens M M, Alford N M, and Klein N, “Microwave Debye relaxation analysis of dissolved proteins: Towards free-solution biosensing”, Appl. Phys. Lett. 99, 233703 (2011).
Back to article

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DOI: https://doi.org/10.21307/ijssis-2019-038 | Journal eISSN: 1178-5608

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Language: English

Page range: 1 - 5

Published on: Feb 15, 2020

Published by: Macquarie University, Australia

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

insulin,

buffer solution,

whispering gallery mode,

dielectric resonator

Related subjects:

Engineering,

Introductions and overviews,

Engineering, other

© 2020 Ritika Verma, K. S. Daya, published by Macquarie University, Australia
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 7 (2014): Issue 5 (January 2014)