Biodegradation Of 4-Chlorobiphenyl By Pseudomonas synxantha

Dhanjal, Noorpreet Inder Kaur; Cameotra, Swaranjit Singh

doi:10.1515/trser-2015-0028

Abstract

The stabilization and disposal of polychlorinated biphenyls (PCBs) from soil environment and wetland areas is of great concern for health and safety. Wetland remediation with microorganisms is an approach for treating PCBs. A bacterial strain was isolated from hydrocarbon contaminated soil of Ropar, Punjab, able to degrade PCBs under aerobic conditions. The percentage of degradation with 100 mM/ml of 4-chlorobiphenyl was up to 90%. Degradation was monitored by mass spectrometry, high performance liquid chromatography and spectrophotometrically, showing that 4-chlorobiphenyl was degraded almost completely. The bacterial strain was identified as Pseudomonas synxantha by 16sRNA sequencing method. This is the first report of 4-chlorobiphenyl degradation by Pseudomonas synxantha.

References

1. Baxter R. A., Gibert R. E., Lidgett R. A., Mainprize J. H. and Vodden H. A., 1975 – The degradation of polychlorinated biphenyls by microorganisms, Science of The Total Environment, 4, 1, 53-61.10.1016/0048-9697(75)90014-5
Search in Google Scholar
2. Dorn E., Hellwig M., Reineke W. and Knackmuss H. J., 1974 – Isolation and characterization of a 3-chlorobenzoate degrading Pseudomonas, Archives of Microbiology, 99, 1, 61-70.10.1007/BF006962224852581
Search in Google Scholar
3. Faroon O., Jones D. and De Rosa C., 2001 – Effects of polychlorinated biphenyls on the nervous system, Toxicology, 61 out of 87, Public, Environmental and Occupational Health (SCI) 71 out of 162.
Search in Google Scholar
4. Furukawa K., Matsumura F. and Tonomura K., 1978 – Alcaligenes and Acinetobacter strains capable of degrading polychlorinated biphenyls, Agricultural and Biological Chemistry, 42, 3, 543-548.10.1271/bbb1961.42.543
Search in Google Scholar
5. Furukawa K., Tomizuka N. and Kamibayashi A., 1983 – Metabolic breakdown of kaneclors (polychlorobiphenyls) and their products by Acinetobacter sp., Applied Environmental and Public Health Microbiology, 46, 1, 140-145.10.1128/aem.46.1.140-145.19832392796412627
Search in Google Scholar
6. Furukawa K., Tonomura K. and Kamibayashi A., 1978 – Effect of chlorine substitution on the biodegradability of polychlorinated biphenyls, Applied Environmental and Public Health Microbiology, 35, 223-227.10.1128/aem.35.2.223-227.197824281516345265
Search in Google Scholar
7. Jong-Chan C., Eunheui K., Sang-Ho P. and Chi-Kyung K., 2000 – Catabolic Degradation of 4-Chlorobiphenyl by Pseudomonas sp. DJ-12 via Consecutive Reaction of meta-cleavage and hydrolytic dechlorination, Biotechnology and Bioprocess Engineering, 5, 6, 449-455.10.1007/BF02931946
Search in Google Scholar
8. Masse R., Messier R., Peloquin L., Ayotte C. and Sylvestre M., 1984 – Microbial biodegradation of 4-chlorobiphenyl, a model compound of chlorinated biphenyls, Applied Environmental and Public Health Microbiology, 47, 947-951.10.1128/aem.47.5.947-951.19842400226742836
Search in Google Scholar
9. Mayes B. A., McConnell E. E., Neal B. H., Brunner M. J., Hamilton S. B., Sullivan T. M., Peters A. C., Ryan M. J., Toft J. D., Singer A. W., Brown J. F., Mentón R. G. and Moore J. A., 1998 – Comparative carcinogenicity in Sprague-Dawley rats of the polychlorinated biphenyl mixtures Aroclors 1016, 1242, 1254 and 1260, Toxicological Sciences, 41, 1, 62-76.10.1006/toxs.1997.239771072299520342
Search in Google Scholar
10. Palma-Fleming H., Cornejo C., González M., Pérez V., González M., Gutierrez E., Sericano J. L. and Seeger M., 2008 – Polycyclic aromatic hydrocarbons and polychlorinated biphenyls from the coastal reef of Valdivia and Valparaiso regions, Chile, Environmental science and Technology, 53, 1393-1398.10.4067/S0717-97072008000200020
Search in Google Scholar
11. Parsons J. R., Sum D. T. H. M., van Laar A. and Hutzinger O., 1988 – Biodegradation of chlorinated biphenyls and benzoic acids by a Pseudomonas species, Applied Microbiology and Biotechnology, 29, 82-84.10.1007/BF00258355
Search in Google Scholar
12. Seeger M. and Pieper D. H., 2009 – Genetics of biphenyl biodegradation and co-metabolism of PCBs in Microbiology of hydrocarbons, oils, lipids, and derived compounds, Timmis K. N. (ed.), Handbook of Hydrocarbon and Lipid Microbiology, 2, 1179-1199.10.1007/978-3-540-77587-4_82
Search in Google Scholar
13. Sklarew D. S. and Girvin D. C., 1987 – Attenuation of polychlorinated biphenyls in soils, Reviews of Environmental Contamination and Toxicology, 98, 1-41.10.1007/978-1-4612-4700-5_13107089
Search in Google Scholar
14. Sylvestre M., Masse R., Ayotte C., Messier F. and Fautex J., 1985 – Total biodegradation of 4-chlorobiphenyl (4 CB) by a two-membered bacterial culture, Applied Microbiology and Biotechnology, 21, 192-195.10.1007/BF00295120
Search in Google Scholar
15. Sylvestre M., Mailhiot K. and Ahmad D., 1989 – Isolation and preliminary characterization of a 2-chlorobenzoate degrading Pseudomonas, Canadian Journal of Microbiology, 35, 439-443.10.1139/m89-0672743216
Search in Google Scholar
16. Yagi O. and Sudo R., 1980 – Degradation of polychlorinated biphenyls by microorganisms, Journal Water Pollution Control Federation, 52, 1035-1043.
Search in Google Scholar

Biodegradation Of 4-Chlorobiphenyl By Pseudomonas synxantha

Abstract

Paradigm

My account