Short Note: Isolation and Characterization of 12 Polymorphic Microsatellite Markers in Engelhardia roxburghiana (Juglandaceae)

Zhang, Dan-Dan; Luo, Pi; Chen, Ying; Wang, Zheng-Feng; Ye, Wan-Hui; Cao, Hong-Lin

doi:10.1515/sg-2014-0014

Abstract

Engelhardia roxburghiana is a common half evergreen tree with a wide distribution in southeast Asia. Despite its ecological and pharmaceutical values, its genetic diversity is poorly studied. Our objective was to develop nuclear microsatellite markers to investigate the level of genetic diversity within and among populations in the future. Using the microsatellite-enriched library and PCR-based screening method, 12 microsatellite markers were developed and showed polymorphism in a population. The number of alleles per locus for these 12 microsatellites ranged from four to 15. The observed and expected heterozygosities ranged from 0.358 to 0.897 and from 0.369 to 0.886, respectively. The developed microsatellites will be useful for studying genetic diversity and population structure in E. roxburghiana.

References

DOYLE, J. J. (1991): DNA protocols for plants - CTAB total DNA isolation, pp. 283-293. In: Molecular techniques in taxonomy, edited by G. M. HEWITT and A. JOHNSTON, Springer-Verlag, Berlin, Germany.10.1007/978-3-642-83962-7_18
Search in Google Scholar Back to article
HOLM, S. (1979): A simple sequentially rejective multiple test procedure. Scandinavian Journal of Statistics 6: 65-70.
Search in Google Scholar Back to article
LU, A.-M., D. E. STONE and L. J. GRAUKE (1999): Juglandaceae, pp. 278. In: Flora of China, Vol. 4, edited by Z.- Y. WU and P. H. RAVEN, Science Press, Beijing, China and Missouri Botanical Garden Press, St. Louis. USA.
Search in Google Scholar Back to article
LUNT, D. H., W. F. HUTCHINSON and G. R. CARVALHO (1999): An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Molecular Ecology 8: 891-894.
Search in Google Scholar Back to article
MANOS, P. S. and D. E. STONE (2001): Evolution, phylogeny, and systematics of the Juglandaceae. Annals of the Missouri Botanical Garden 88: 231-269.10.2307/2666226
Search in Google Scholar Back to article
ROUSSET, F. (2008): GENEPOP’007: A complete reimplementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8: 103-106.10.1111/j.1471-8286.2007.01931.x21585727
Search in Google Scholar Back to article
ROZEN, S. and H. SKALETSKY (2000): Primer3 on the WWW for general users and for biologist programmers, pp. 365-386. In: Methods in molecular biology, vol. 132: Bioinformatics methods and protocols, edited by S. MISENER and S. A. KRAWETZ, Humana Press, Totowa, New Jersey, USA.10.1385/1-59259-192-2:365
Search in Google Scholar Back to article
WU, C. C., C. F. PENG, I. L. TSAI, M. H. ABD EL-RAZEK, H. S. HUANG and I. S. CHEN (2007): Secondary metabolites from the roots of Engelhardia roxburghiana and their antitubercular activities. Phytochemistry 68: 1338-1343.10.1016/j.phytochem.2007.01.01817418287
Search in Google Scholar Back to article
WU, H. C., M. J. CHENG, C. F. PENG, S. C. YANG, H. S. CHANG, C. H. LIN, C. J. WANG and I. S. CHEM (2012): Secondary metabolites from the stems of Engelhardia roxburghiana and their antitubercular activities. Phytochemistry 82: 118-127.10.1016/j.phytochem.2012.06.01422818359
Search in Google Scholar Back to article
XIN, W., H. HUANG, L. YUA, H. SHIB, Y. SHENG, T. T.Y. WAN and L. YU (2012): Three new flavanonol glycosides from leaves of Engelhardtia roxburghiana, and their antiinflammation, antiproliferative and antioxidant properties. Food Chemistry 132: 788-798.10.1016/j.foodchem.2011.11.038
Search in Google Scholar Back to article

Short Note: Isolation and Characterization of 12 Polymorphic Microsatellite Markers in Engelhardia roxburghiana (Juglandaceae)

Abstract

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