Short Note: Isolation and Characterization of Microsatellite Loci in Castanopsis fissa in Lower Subtropical China

Lei Dong; Zheng-Feng Wang; Peng Zhu; Wan-Hui Ye

doi:10.1515/sg-2010-0042

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Short Note: Isolation and Characterization of Microsatellite Loci in Castanopsis fissa in Lower Subtropical China

Silvae Genetica

Volume 59 (2010): Issue 1-6 (December 2010)

By: Lei Dong, Zheng-Feng Wang, Peng Zhu and Wan-Hui Ye

Open Access

|Oct 2017

Abstract

We report on the development and characterization of ten microsatellite markers from repetitive DNA enriched libraries for Castanopsis fissa from lower subtropical China. The number of alleles ranged from three to thirteen. Observed and expected heterozygosities ranged from 0.265 to 0.818, and 0.270 to 0.873, respectively. These microsatellite markers will be used to study fine-scale spatial genetic structure of C. fissa in 20 ha Dinghushan plot in lower subtropical China.

References

CORNELISSEN, J. H. C. (1993): Aboveground morphology of shade-tolerant Castanopsis fargesii saplings in respond to light environment. International Journal of Plant Sciences 154: 481-495.10.1086/297132
Search in Google Scholar Back to article
DOYLE, J. J. (1991): DNA protocols for plants - CTAB total DNA isolation, pp. 283-293. In: Molecular Techniques in Taxonomy, edited by G. M. HEWITT and A. JOHNSTON, Springer-Verlag, Berlin, Germany.10.1007/978-3-642-83962-7_18
Search in Google Scholar Back to article
LEWIS, P. O. and D. ZAYKIN (2001): Genetic Data Analysis: Computer program for the analysis of allelic data. Version 1.0 (d16c). Free program distributed by the authors over the internet from http://lewis.eeb.uconn.edu/lewishome/software.html.
Search in Google Scholar Back to article
LUNT, D. H., W. F. HUTCHINSON and G. R. CARVALHO (1999): An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Molecular Ecology 8: 891-894.
Search in Google Scholar Back to article
RAYMOND, M. and F. ROUSSET (1995): GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity 86: 248-249.10.1093/oxfordjournals.jhered.a111573
Search in Google Scholar Back to article
ROUSSET, F. (2008): Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Molecular Ecology Resources 8: 103-106.10.1111/j.1471-8286.2007.01931.x21585727
Search in Google Scholar Back to article
TAM, P. C. F. and D. A. GRIFFITHS (1994): Mycorrhizal associations in Hong Kong Fagaceae. VI. Growth and nutrient uptake by Castanopsis fissa seedlings inoculated with ectomycorrhizal fungi. Mycorrhiza 4: 169-172.10.1007/BF00203535
Open DOI Search in Google Scholar Back to article
WEIR, B. S. and C. C. COCKERHAM (1984): Estimating F-statistics for the analysis of population structure. Evolution 38: 1358-1370.10.2307/2408641
Search in Google Scholar Back to article

Articles in this issue

DOI: https://doi.org/10.1515/sg-2010-0042 | Journal eISSN: 2509-8934 | Journal ISSN: 0037-5349

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Language: English

Page range: 299 - 300

Submitted on: Nov 22, 2010

Published on: Oct 20, 2017

Published by: Johann Heinrich von Thünen Institute

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

lower subtropical China,

reforestation,

spatial genetic structure,

marker development,

DNA enriched libraries,

Hardy-Weinberg equilibrium,

linkage disequilibrium,

Related subjects:

© 2017 Lei Dong, Zheng-Feng Wang, Peng Zhu, Wan-Hui Ye, published by Johann Heinrich von Thünen Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 59 (2010): Issue 1-6 (December 2010)