Construction of a Populus tremuloides Michx. BAC library

Fladung, M.; Kaufmann, H.; Markussen, T.; Hoenicka, H.

doi:10.1515/sg-2008-0010

Abstract

We have constructed an aspen (Populus tremuloides Michx., line Turesson141) BAC library containing 55,296 clones in total. A random sampling of 86 BAC clones indicated an average insert size of 76 kb with a range of 20 to 160 kb. Twelve percent of the BAC clones in the library have an insert size larger than 100 kb. Based on an estimated genome size for Populus of 500 Mbp, library coverage is about 8 haploid genome equivalents. This library will be screened using AFLP marker identified before co-segregating with gender in a P. tremula x P. tremuloides progeny, where Turesson141 was the male parent.

References

ALLOUIS, S., X. QI, M. D. GALE and K. M. DEVOS (2001): Construction of a BAC library of pearl millet, Pennisetum glaucum. Theor. Appl. Genet. 102: 1200-1205.10.1007/s001220100559
Open DOI Search in Google Scholar Back to article
BHALERAO, R., O. Nilsson and G. Sandberg (2003): Out of the woods: forest biotechnology enters the genomic era. Curr. Opin. Biotechnol. 14: 206-213.10.1016/S0958-1669(03)00029-6
Open DOI Search in Google Scholar Back to article
BRAATNE, J. H. (1999): Biological aspects of hybrid poplar cultivation on floodplains in western North America. Prepared for U.S. EPA (Region 10), Seattle, WA, Document No. 910-R-99-002.
Search in Google Scholar Back to article
CERVERA, M. T., M. M. SEWELL, P. FAIVRE-RAMPANT, V. STORME and W. BOERJAN (2004): Genome mapping in Populus. In: KUMAR, S., FLADUNG, M. (Eds) Molecular Genetics and Breeding of Forest Trees. The Haworth Press, Binghamton, 387-410.
Search in Google Scholar Back to article
CERVERA, M. T., V. STORME, B. IVENS, J. GUSMÃO, B. H. LIU, V. HOSTYN, J. VAN SLYCKEN, M. VAN MONTAGU and W. BOERJAN (2001): Dense genetic linkage maps of three Populus species (Populus deltoides, P. nigra and P. trichocarpa) based on AFLP and microsatellite markers. Genetics 158: 787-809.10.1093/genetics/158.2.787
Search in Google Scholar Back to article
CLARKE, L. and J. CARBON (1976): A colony bank containing synthetic Col EI hybrid plasmids representative of the entire E. coli genome. Cell 9: 91-99.10.1016/0092-8674(76)90055-6
Open DOI Search in Google Scholar Back to article
DICKMAN, D. I. and K. W. STUART (1983): The Culture of Poplars in Eastern North America. Department of Forestry Publications, Michigan State University, East Lansing, Michigan.
Search in Google Scholar Back to article
FAIVRE RAMPANT, P., L. BATAILLE, A. BRESSON, N. BOUDET, B. CHALHOUB, V. JORGE, A. DOWKIW, V. GUERIN, J. P. MASLE, M. VILLAR and C. BASTIEN (2006): Genetic and Physical mapping of a genetic factor involving in partial rust resistance in Populus trichocarpa. IV International Poplar Symposium, Nanjing, China.
Search in Google Scholar Back to article
GRATTAPAGLIA, D. (2004): Integrating genomics into Eucalyptus breeding. Genet. Mol. Res. 3: 369-379.
Search in Google Scholar Back to article
KAUFMANN, H., L. MATTIESCH, H. LÖRZ and T. DEBENER (2003): Construction of a BAC library of Rosa rugosa Thunb. And assembly of a contig spanning Rdr1, a gene that confers resistance to blackspot. Mol. Genet. Genomics 268: 666-674.
Search in Google Scholar Back to article
KIM, U. J., B. W. BIRREN, T. SLEPAK, V. MANCINO, D. BOYSEN, H. L. KANG, M. I. SIMON and H. SHIZUYA (1996): Construction and characterization of a human bacterial artificial chromosome library. Genomics 34: 213-218.10.1006/geno.1996.02688661051
Open DOI Search in Google Scholar Back to article
NOIR, S., S. PATHEYRON, M. C. COMBES, P. LASHERMES and B. CHALHOUB (2004): Construction and characterisation of a BAC library for genome analysis of the allotetraploid coffee species (Coffea arabica L.). Theor. Appl. Genet. 109: 225-230.
Search in Google Scholar Back to article
PATOCCHI, A., B. A. VINATZER, L. GIANFRANCESCI, S. TARTARINI, H. B. ZHANG, S. SANSAVINI and C. GESSLER (1999): Construction of a 550-kb BAC contig spanning the genomic region containing the apple scab resistance gene Vf. Mol. Genet. Genomics 262: 884-891.
Search in Google Scholar Back to article
PETERSON, D. G., J. P. TOMKINS, D. A. FRISCH, R. A. WING and A. H. PATERSON (2000): Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide. JAG 5: www.ncgr.org/research/jag.
Search in Google Scholar Back to article
SAMBROOK, J., E. F. FRITSCH and T. MANIATIS (1989): Molecular cloning: a laboratory manual, 2nd Edn, Cold Spring Harbour Laboratory Press, New York.
Search in Google Scholar Back to article
STETTLER, R. F., H. D. BRADSHAW, P. E. HEILMAN and T. M. HINCKLEY (1996): Biology of Populus: Implications for management and conservation. National Research Council of Canada, Ottawa.
Search in Google Scholar Back to article
STIRLING, B., G. NEWCOMBE, J. VREBALOV, I. Bosdet and H. D. BRADSHAW (2001): Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust. Theor. Appl. Genet. 103: 1129-1137.
Search in Google Scholar Back to article
STRONG, S. J., Y. OHTA, G. W. LITMAN and C. T. AMEMIYA (1997): Marked improvement of PAC and BAC cloning is achieved using electroelution of pulsed-field gel-separated partial digests of genomic DNA. Nucleic Acids Res. 25: 3959-3961.10.1093/nar/25.19.39591469819380525
Open DOI Search in Google Scholar Back to article
TOMKINS, J. P., D. G. PETERSON, T. J. YANG, D. MAIN, E. F. ABLETT, R. J. HENRY, L. S. LEE, T. A. HOLTON, D. WATERS and R. A. WING (2001): Grape (Vitis vinifera L.) BAC library construction, preliminary STC analysis, and identification of clones associated with flavonoid and stilbene biosynthesis. Amer. J. Enomol. Viticult. 52: 287-291.10.5344/ajev.2001.52.4.287
Search in Google Scholar Back to article
VILANOVA, S., C. ROMERO, D. ABERNATHY, A. G. ABBOTT, L. BURGOS, G. LLACER and M. L. BADENES (2003): Construction and application of a bacterial artificial chromosome (BAC) library of Prunus armeniaca L. for the identification of clones linked to the self-incompatibility locus. Mol. Genet. Genomics 269: 685-691.
Search in Google Scholar Back to article
VINATZER, B. A., H. B. ZHANG and S. SANSAVINI (1998): Construction and characterization of a bacterial artificial chromosome library of apple. Theor. Appl. Genet. 97: 1183-1190.10.1007/s001220051008
Open DOI Search in Google Scholar Back to article
WANG, W., W. ZHAI, M. LUO, G. JIANG, X. CHEN, X. LI, R. A. WING and L. ZHU (2001): Chromosome landing at the bacterial blight resistance gene Xa4 locus using a deep coverage rice BAC library. Mol. Genet. Genomics 265: 118-125.
Search in Google Scholar Back to article
XU, M. L., J. Q. SONG, Z. K. CHENG, J. M. JIANG and S. S. KORBAN (2001): A bacterial artificial chromosome (BAC) library of Malus floribunda 821 and contig construction for positional cloning of the apple scab resistance gene Vf. Genome 44: 1104-1113.10.1139/g01-105
Search in Google Scholar Back to article
YIN, T. M., M. R. HUANG, M. X. WANG, L. H. ZHU, Z. B. ZENG and R. L. WU (2001): Preliminary interspecific genetic maps of the Populus genome constructed from RAPD markers. Genome 44: 602-609.10.1139/g01-054
Open DOI Search in Google Scholar Back to article
YIN, T. M., X. Y. ZHANG, M. R. HUANG, M. X. WANG, Q. ZHUGE, S. M. TU, L. H. ZHU and R. L. WU (2002): Molecular linkage maps of the Populus genome. Genome 45: 541-555.10.1139/g02-01312033623
Open DOI Search in Google Scholar Back to article
YOKOYA, K., A.V. ROBERTS, J. MOTTLEY, R. LEWIS and P. E. BRANDHAM (2000): Nuclear DNA amounts in roses. Ann. Bot. 85: 557-561.
Search in Google Scholar Back to article
ZHU, H. and R. A. DEAN (1999): A novel method for increasing the transformation efficiency of E. coli - application for bacterial artificial chromosome library construction. Nucleic Acids Res. 27: 910-912.10.1093/nar/27.3.9101482659889291
Open DOI Search in Google Scholar Back to article

Construction of a Populus tremuloides Michx. BAC library

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