Segregation and Linkage Relationships of Allozymes in Pinus Brutia Ten

Kaya, Nuray; Isik, K.

doi:10.1515/sg-2006-0022

Abstract

Female gametophytes of Pinus brutia Ten. were analyzed to study Mendelian segregation and linkage among allozyme loci in a seed orchard composed of 28 clones. Isozyme variants of nine enzyme systems encoding 14 loci from megagametophytes were assayed by starch gel electrophoresis. While six of the 14 loci were monomorphic, the remaining eight were polymorphic. Analysis of observed segregation ratios of all polymorphic loci except for Sdh1, showed a good fit to the 1:1 ratios expected for Mendelian inheritance. Linkage relationships of the eight polymorphic allozyme loci were analyzed by testing 23 of the 28 possible two-locus combinations. Two-locus segregations in three pairs (Got2- Mdh1, Got2-Pgi2 and Pgd3-Sdh1) were significantly heterogeneous (P ≤ 0.05). Relatively weak linkage was observed between only one pair of genes (Adh2:Got2). Recombination fraction for Adh2:Got2 was found to be 0.24 (± 0.06).

References

ADAMS, W. T. and R. J. JOLY (1980): Linkage relationships among twelve enzyme loci in loblolly pine. The Journal of Heredity 71: 199-202.10.1093/oxfordjournals.jhered.a109347
Search in Google Scholar Back to article
ADAMS, W. T., D. B. NEALE, A. H. DOERKSEN and D. B. SMITH (1990): Inheritance and linkage of isozyme variants from seed and vegetative bud tissues in coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco]. Silvae Genetica 39: 153-167.
Search in Google Scholar Back to article
BOYLE, T. J. and E. K. MORGENSTERN (1985): Inheritance and linkage relationships of some isozymes of black spruce in New Brunswick. Can. J. For. Res. 15: 992-996.
Search in Google Scholar Back to article
BROWN, A. H. D., A. C. MATHESON and K. G. ELDRIDGE (1975): Estimation of the mating system of Eucalyptus obliqua L’Herit. by using allozyme polymorphisms. Aust. J. Bot. 28: 931-949.
Search in Google Scholar Back to article
CHELIAK, W. M., K. MORGAN, B. P. DANCIK, C. STROBECK and F. C. H. YEH (1984): Segregation of allozymes in megagametophytes of viable seed from a natural population of jack pine, Pinus banksiana Lamb. Theor. Appl. Genet. 69: 145-151.
Search in Google Scholar Back to article
CHELIAK, W. M. and J. A. PITEL (1985): Inheritance and linkage of allozymes in Larix laricina. Silvae Genetica 34: 142-148.
Search in Google Scholar Back to article
FAROOQ, S. and F. AZAM (2002). Molecular markers in plant breeding-II. Some prerequsities for use. Pakistan J. of Biol. Sci. 5(10): 1141-1147.
Search in Google Scholar Back to article
FURNIER, G. R., P. KNOWLES, M. A. ALEKSIUK and B. P. DANCIK (1986): Inheritance and linkage of allozymes in seed tissue of white bark pine. Can. J. Genet. Cytol. 28: 601-604. 10.1139/g86-088
Open DOI Search in Google Scholar Back to article
GEBUREK, T. and G. VON WUEHLISCH (1989): Linkage analysis of isozyme gene loci in Picea abies (L.) Karst. Heredity 62: 185-191.10.1038/hdy.1989.27
Open DOI Search in Google Scholar Back to article
KARA, N., L. KOROL, K. ISIK and G. SCHILLER (1997): Genetic Diversity in Pinus brutia Ten.: Altitudinal variation. Silvae Genetica 46 (2-3): 155-161.
Search in Google Scholar Back to article
KAYA, N. (2001): Estimation of Mating System and Pollen Contamination in a Clonal Seed Orchard of Pinus brutia Ten. in Asar-Antalya. Ph. D. Thesis, Akdeniz Univ., Graduate School of Natural and Applied Sciences, 81 pp. (in Turkish with English summary).
Search in Google Scholar Back to article
KAYA, N., K. ISIK and W. T. ADAMS (2005): Mating system and pollen contamination in a Pinus brutia seed orchard. New Forest 00:1-8. DOI 10.1007/s11056-005- 0876-x10.1007/s11056-005-0876-x
Open DOI Search in Google Scholar Back to article
NEALE, D. B. and W. T. ADAMS (1981): Inheritance of isozyme variants in seed tissues of balsam fir (Abies balsamea). Can. J. Bot. 59: 1285-1291.
Search in Google Scholar Back to article
NIEBLING, C. R., K. JOHNSON and H. D. GERHOLD (1987): Electrophoretic analysis of genetic linkage in Scots pine (Pinus sylvestris L.). Biochemical Genetics 25 (11-12): 803-814. 10.1007/BF005026003450273
Open DOI Search in Google Scholar Back to article
RUDIN, D. and I. EKBERG (1978): Linkage studies in Pinus sylvestris L.-using macro gametophyte allozymes. Silvae Genetica 27: 1-12.
Search in Google Scholar Back to article
SHAW, D. V. and R.W. ALLARD (1981): Analysis of mating system parameters and population structure in Douglas- fir using single-locus and multi-locus methods, pp. 18-22. In: Proc. Symp. Isozymes North Am. Forest Trees and Forest Insects edited by CONKLE, M. T., BERKELEY, C. A. USDA Forest Serv. Gen. Techn. Rep. PSW-48.
Search in Google Scholar Back to article
STRAUSS, S. H. and M. T. CONKLE (1986): Segregation, linkage and diversity of allozymes in knobcone pine. Theor. Appl. Genet. 72: 483-493.
Search in Google Scholar Back to article
SZMIDT, A. E. and O. MUONA (1989): Linkage relationships of allozyme loci in Pinus sylvestris. Hereditas 111: 91-97.10.1111/j.1601-5223.1989.tb00382.x
Open DOI Search in Google Scholar Back to article

Segregation and Linkage Relationships of Allozymes in Pinus Brutia Ten

Abstract

Paradigm

My account