Down-regulation of miRNA-30c predicts poor prognosis in Colorectal Cancer patients

Bin, Liu; Zhang, Meng; Lixia, Liu; Aimin, Zang; Hua, Yang; Yanhong, Shang; Yang, Yang; Feng, Gao; Bo, Liu; Yonggang, Zhang; Huiping, Tian

doi:10.1515/rrlm-2016-0037

Abstract

Background: MiRNA-30c was a tumor suppressor in several human cancers, however, its association with clinicopathological features and prognosis in colorectal cancer (CRC) is unclear.

Materials and Methods: The expression level of miRNA-30c in 192 pairs of colorectal cancer and adjacent normal tissues was detected by Quantitative RT-PCR, the association between miRNA-30c expression and clinical characteristics and prognosis were statistically analyzed.

Results: miRNA-30c was significantly lower in CRC tissues specimens compared with matched normal adjacent tissue (P<0.001). MiRNA-30c was positively correlated with tumor size (P=0.012), TMN stage (P=0.002) and lymph node metastasis (P=0.004). The univariate analysis showed CRC patients with low miRNA-30c had distinctly shorter overall survival (P<0.001) than patients with high miRNA-30c expression level. The multivariate analysis was performed and informed that low miRNA-30c expression (P<0.001) might be an independent prognostic predictor for poor prognosis.

Conclusion: miRNA-30c could predict the prognosis of colorectal cancer which is helpful to choose reasonable treatment measures.

References

1. Parkin DDM. Global cancer statistics in the year 2000. Lancet Oncol. 2001;2(9):533-43. DOI: 10.1016/S1470-2045(01)00486-7.10.1016/S1470-2045(01)00486-7
Search in Google Scholar Back to article
2. Ng SC, Wong SH. Colorectal cancer screening in Asia. Br Med Bull. 2013;105(1):29-42. DOI: 10.1093/bmb/lds040.10.1093/bmb/lds040
Search in Google Scholar Back to article
3. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T. Identification of novel genes coding for small expressed RNAs. Science. 2001;294(5543):853-8. DOI: 10.1126/science.1064921.10.1126/science.1064921
Search in Google Scholar Back to article
4. Liu M, Chen H. The role of microRNAs in colorectal cancerr. J Genet Genomics. 2010;37(6):347-58. DOI: 10.1016/S1673-8527(09)60053-9.10.1016/S1673-8527(09)60053-9
Search in Google Scholar Back to article
5. Wang H, An H, Wang B, Liao Q, Li W. miR-133a represses tumour growth and metastasis in colorectal cancer by targeting LIM and SH3 protein 1 and inhibiting the MAPK pathway. Eur J Cancer. 2013;49(18):3924-35. DOI: 10.1016/j.ejca.2013.07.149.10.1016/j.ejca.2013.07.14923968734
Search in Google Scholar Back to article
6. Wu ZS, Wang CQ, Ru X, Xue L, Shan Y, Yang XQ, et al. Loss of miR-133a expression associated with poor survival of breast cancer and restoration of miR-133a expression inhibited breast cancer cell growth and invasion. Bmc Cancer. 2012;12(1):1-10. DOI: 10.1186/1471-2407-12-51.10.1186/1471-2407-12-51329752722292984
Search in Google Scholar Back to article
7. Wu W, Yang P, Feng X, Wang H, Qiu Y, Tian T, et al. The relationship between and clinical significance of MicroRNA-32 and phosphatase and tensin homologue expression in colorectal cancer. Genes Chromosomes Cancer. 2013;52(12):1133-40. DOI: 10.1002/gcc.22108.10.1002/gcc.2210824123284
Search in Google Scholar Back to article
8. Nagel R, Le SC, Diosdado B, Van dWM, Oude Vrielink JA, Bolijn A, et al. Regulation of the adenomatous polyposis coli gene by the miR-135 family in colorectal cancer. Cancer Res. 2008;68(14):5795-802. DOI: 10.1158/0008-5472.CAN-08-0951.10.1158/0008-5472.CAN-08-095118632633
Search in Google Scholar Back to article
9. O’Connor SM, Young GP, Van HP, N G, James RJ, Michael MZ. Reduced accumulation of specific MicroRNAs colorectal neoplasia; colorectal neoplasia. Oncol & Carcinog. 2003.
Search in Google Scholar Back to article
10. Zhang GJ, Zhou H, Xiao HX, Li Y, Zhou T. MiR-378 is an independent prognostic factor and inhibits cell growth and invasion in colorectal cancer. Bmc Cancer. 2014;14(6):109. DOI: 10.1186/1471-2407-14-109.10.1186/1471-2407-14-109397411424555885
Search in Google Scholar Back to article
11. Li H, Dai S, Zhen T, Shi H, Zhang F, Yang Y, et al. Clinical and biological significance of miR-378a-3p and miR-378a-5p in colorectal cancer. Eur J Cancer. 2014;50(6):1207–21. DOI: 10.1016/j.ejca.2013.12.010.10.1016/j.ejca.2013.12.01024412052
Search in Google Scholar Back to article
12. Rodríguez-González FG, Sieuwerts AM, Smid M, Look MP, Gelder MV, Weerd VD, et al. MicroRNA-30c expression level is an independent predictor of clinical benefit of endocrine therapy in advanced estrogen receptor positive breast cancer. Breast Cancer Res Treat. 2011;127(1):43-51. DOI: 10.1007/s10549-010-0940-x.10.1007/s10549-010-0940-x20490652
Search in Google Scholar Back to article
13. Budhu A, Jia H-L, Forgues M, Liu CG, Goldstein D, Lam A, et al. Identification of metastasis-related microRNAs in hepatocellular carcinoma. Hepatology. 2008;47(3):897–907. DOI: 10.1002/hep.22160.10.1002/hep.2216018176954
Search in Google Scholar Back to article
14. Huang Z, Long Z, Yi X, Yu X. Diagnostic and prognostic values of tissue hsa-miR-30c and hsa-miR-203 in prostate carcinoma. Tumour Biol. 2015:1-7. DOI: 10.1007/s13277-015-4262-9.10.1007/s13277-015-4262-926499781
Search in Google Scholar Back to article
15. Edge SB, Byrd DR, Compton CC, Fritz AG, Greene FL, Trotti A, editors. AJCC cancer staging manual (7th ed). New York, NY: Springer; 2010.
Search in Google Scholar Back to article
16. Huang B, Feng Y, Zhu L, Xu T, Huang L, Cai G. Smaller tumor size is associated with poor survival in stage II colon cancer: An analysis of 7,719 patients in the SEER database. Int J Surg.2016; 33(Pt A):157-63. DOI: 10.1016/j.ijsu.2016.07.073.10.1016/j.ijsu.2016.07.07327491969
Search in Google Scholar Back to article
17. Amirkhah R, Farazmand A, Irfanmaqsood M, Wolkenhauer O, Wolkenhauer O, Schmitz U. The role of microRNAs in the resistance to colorectal cancer treatments. Cell Mol Biol. 2015;61(6):17-23. DOI: 10.14715/cmb/2015.61.6.4.10.14715/cmb/2015.61.6.4
Search in Google Scholar Back to article
18. Peng G, Yin Z, Li X, Wei W, Zhou B. Meta-analysis of human lung cancer microRNA expression profiling studies comparing cancer tissues with normal tissues. J Exp Clin Cancer Res. 2012;31(1):1-8. DOI: 10.1186/1756-9966-31-54.10.1186/1756-9966-31-54350208322672859
Search in Google Scholar Back to article
19. Tanic M, Yanowsky K, Rodriguezantona C, Andrés R, Márquezrodas I, Osorio A, et al. Deregulated miRNAs in hereditary breast cancer revealed a role for miR-30c in regulating KRAS oncogene. Plos One. 2012;7(6). DOI: 10.1371/journal.pone.0038847.10.1371/journal.pone.0038847337246722701724
Search in Google Scholar Back to article
20. Quintavalle C, Donnarumma E, Iaboni M, Roscigno G, Garofalo M, Romano G, et al. Effect of miR-21 and miR-30b/c on TRAIL-induced apoptosis in glioma cells. Oncogene. 2012;31(34):4001–8. DOI: 10.1038/onc.2012.410.10.1038/onc.2012.41022964638
Search in Google Scholar Back to article
21. Ichikawa T, Sato F, Terasawa K, Tsuchiya S, Toi M, Tsujimoto G, et al. Trastuzumab produces therapeutic actions by upregulating miR-26a and miR-30b in breast cancer cells. Plos One. 2012;7(2):e31422. DOI: 10.1371/journal.pone.0031422.10.1371/journal.pone.0031422328804322384020
Search in Google Scholar Back to article

Down-regulation of miRNA-30c predicts poor prognosis in Colorectal Cancer patients

Abstract

Paradigm

My account