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PCR coupled with mass-spectrometry for detection of Clostridium difficile virulence markers during the emergence of ribotype 027 in Bucharest area Cover

PCR coupled with mass-spectrometry for detection of Clostridium difficile virulence markers during the emergence of ribotype 027 in Bucharest area

Revista Romana de Medicina de Laborator
Volume 23 (2015): Issue 4 (December 2015)
By: ,  ,  ,  ,  ,   and    
Open Access
|Dec 2015
References

References

  1. 1. Bauer MP, Notermans DW, van Benthem BH, Brazier JS, Wilcox MH, Rupnik M, et al.infection in Europe: a hospital-based survey. Lancet. 2011;377:63-73. DOI: 10.1016/S0140-6736(10)61266-4
    Open DOISearch in Google Scholar
  2. 2. Vohra P, Poxton IR. Comparison of toxin and spore production in clinically relevant strains of. Microbiology. 2011;157:1343-53. DOI: 10.1099/mic.0.046243-0
    Open DOISearch in Google Scholar
  3. 3. Goorhuis A, Bakker D, Corver J, Debast SB, Harmanus C, Notermans DW, et al. Emergence ofinfection due to a new hypervirulent strain, polymerase chain reaction ribotype 078. Clin Infect Dis. 2008;47:1162-70. DOI: 10.1086/592257
    Open DOISearch in Google Scholar
  4. 4. Stabler RA, He M, Dawson LFT, Martin M, Valiente E, Parkhill J, et al Comparative genome and phenotypic analysis of027 strains provides insight into the evolution of a hypervirulent bacterium. Genome Biol. 2009;10:R102. DOI: 10.1186/gb-2009-10-9-r102
    Open DOISearch in Google Scholar
  5. 5. Lim SK, Stuart RL, Mackin KE, Carter GP, Kotsanas D, Francis MJ, et al. Emergence of a ribotype 244 strain ofassociated with severe disease and related to the epidemic ribotype 027 strain. Clin Infect Dis. 2014;58:1723-30. DOI: 10.1093/cid/ciu203
    Open DOISearch in Google Scholar
  6. 6. Rafila A, Indra A, Popescu GA, Wewalka G, Allerberger F, Benea S, et al. Occurrence ofinfections due to PCR ribotype 027 in Bucharest, Romania. J Infect Dev Ctries. 2014;8:694-8. DOI: 10.3855/jidc.4435
    Open DOISearch in Google Scholar
  7. 7. Indra A, Huhulescu S, Schneeweis M, Hasenberger P, Kernbichler S, Fiedler A, et al. Characterization ofisolates using capillary gel electrophoresis –based PCR ribotyping. J Med Microbiol. 2008;57:1377-82. DOI: 10.1099/jmm.0.47714-0
    Open DOISearch in Google Scholar
  8. 8. van den Berg RJ, Claas ECJ, Oyib DH, Klaassen CHW, Dijkshoorn L, Brazier JS, et al. Characterization of toxin A-negative, toxin B-positiveisolates from outbreaks in different countries by amplified fragment length polymorphism and PCR ribotyping. J Clin Microbiol. 2004;42:1035–41. DOI: 10.1128/JCM.42.3.1035-1041.2004
    Open DOISearch in Google Scholar
  9. 9. Kato N, Ou CY, Kato H, Bartley SL, Brown VK, Dowell VRJ, Jr, et al. Identification of toxigenicby the polymerase chain reaction. J Clin Microbiol. 1991;29:33–7.
    Open DOISearch in Google Scholar
  10. 10. Kato H, Kato N, Katow S, Maegawa T, Nakamura S, Lyerly DM. Deletions in the repeating sequences of the toxin A gene of toxin A-negative, toxin B-positivestrains. FEMS Microbiol Lett. 1999;175:197–203. DOI: 10.1111/j.1574-6968.1999. tb13620.x
    Search in Google Scholar
  11. 11. Stubbs SL, Brazier JS, Talbot PR, Duerden BI. PCR-restriction fragment length polymorphism analysis for identification ofspp. and characterization of nitroimidazole resistance genes. J Clin Microbiol. 2000;38:3209–13.
    Open DOISearch in Google Scholar
  12. 12. Spigaglia P, Mastrantonio P. Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) amongclinical isolates. J Clin Microbiol. 2002;40:3470–5. DOI: 10.1128/JCM.40.9.3470-3475.2002
    Open DOISearch in Google Scholar
  13. 13. Curry SR, Marsh JW, Muto CA, O’Leary MM, Pasculle AW, Harrison LH. tcdC Genotypes associated with severe tcdC truncation in an epidemic clone and other strains of. J Clin Microbiol. 2007;45:215–21. DOI: 10.1128/JCM.01599-06
    Open DOISearch in Google Scholar
  14. 14. Wolff D, Brüning T, Gerritzen A. Rapid detection of theribotype 027 tcdC gene frame shift mutation at position 117 by real-time PCR and melt curve analysis. Eur J Clin Microbiol Infect Dis. 2009;28:959-62. DOI: 10.1007/s10096-009-0731-7
    Open DOISearch in Google Scholar
  15. 15. Ecker DJ, Sampath R, Blyn LB, Eshoo MW, Ivy C, Ecker JA, et al. Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. Proc Natl Acad Sci U S A. 2005;102:8012-7. DOI: 10.1073/pnas.0409920102
    Open DOISearch in Google Scholar
  16. 16. Wolk DM, Kaleta EJ, Wysocki VH. PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories. J Mol Diagn. 2012;14:295-304. DOI: 10.1016/j.jmoldx.2012.02.005
    Open DOISearch in Google Scholar
  17. 17. Wilcox MH, Shetty N, Fawley WN, Shemko M, Coen P, Birtles A, et al. Changing epidemiology ofinfection following the introduction of a national ribotyping-based surveillance scheme in England. Clin Infect Dis. 2012;55:1056-63. DOI: 10.1093/cid/cis614
    Open DOISearch in Google Scholar
  18. 18. Popescu G, Serban R, Pistol A, Niculcea A, Preda A, Lemeni D, et al. Clinical and microbiological characterization ofinfection in Romania (2013-2014); a hospital-based study, BMC Infect Dis. 2014;14(Suppl 7):O24. DOI: 10.1186/1471-2334-14-S7-O24
    Open DOISearch in Google Scholar
  19. 19. Drudy D, Fanning S, Kyne L. Toxin A-negative, toxin B-positive. Int J Infect Dis. 2007;11:5–10. DOI: 10.1016/j.ijid.2006.04.003
    Open DOISearch in Google Scholar
DOI: https://doi.org/10.1515/rrlm-2015-0044 | Journal eISSN: 2284-5623 | Journal ISSN: 1841-6624 (formerly 2284-5623)
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Language: English, Romanian
Page range: 449 - 456
Submitted on: Aug 21, 2015
Accepted on: Oct 25, 2015
Published on: Dec 30, 2015
Published by: Romanian Association of Laboratory Medicine
In partnership with: Paradigm Publishing Services
Keywords:
Clostridium difficile,
ribotype 027,
binary toxin,
deletion in tcdC gene,
electrospray ionization mass spectrometry
Related subjects:
Life sciences,
Molecular biology

© 2015 Dragos Florea, Steliana Huhulescu, Alexander Indra, Ioana Badicut, Alexandru Rafila, Dan Otelea, Gabriel Adrian Popescu, published by Romanian Association of Laboratory Medicine
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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