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Phytotherapeutics oridonin and ponicidin show additive effects combined with irradiation in pancreatic cancer in vitro Cover

Phytotherapeutics oridonin and ponicidin show additive effects combined with irradiation in pancreatic cancer in vitro

Radiology and Oncology
Volume 51 (2017): Issue 4 (December 2017)
By: Jakob Liermann,  Patrick Naumann,  Franco Fortunato,  Thomas E. Schmid,  Klaus-Josef Weber,  Jürgen Debus and  Stephanie E. Combs  
Open Access
|Nov 2017

Figures & Tables

Figure 1

Clonogenic survival of Panc-1 cells treated with either irradiation and/or oridonin (ORI) and ponicidin (PON) as indicated. (A) survival curves and (B) radiosensitivity to different dosages with measured and theoretical control curve, according to Steel and Peckham for Panc-1 cells. Results are presented as mean values +/− standard deviation. At least three independent experiments were performed.
Clonogenic survival of Panc-1 cells treated with either irradiation and/or oridonin (ORI) and ponicidin (PON) as indicated. (A) survival curves and (B) radiosensitivity to different dosages with measured and theoretical control curve, according to Steel and Peckham for Panc-1 cells. Results are presented as mean values +/− standard deviation. At least three independent experiments were performed.

Figure 2

Cell cycle assay of Panc-1 cells treated with irradiation and/or (A) oridonin (ORI) and (B) ponicidin (PON) as indicated. Results are presented as mean values +/− standard deviation. *P<0.05, **P<0.005, asterisk referring to the amount of cells in G2/M-phase. At least two independent experiments were performed.
Cell cycle assay of Panc-1 cells treated with irradiation and/or (A) oridonin (ORI) and (B) ponicidin (PON) as indicated. Results are presented as mean values +/− standard deviation. *P<0.05, **P<0.005, asterisk referring to the amount of cells in G2/M-phase. At least two independent experiments were performed.

Figure 3

γH2AX-intensity of Panc-1 and MIA PaCa-2 cells treated with irradiation and/or (A) oridonin (ORI) and (B) ponicidin (PON) as indicated. Results are presented as mean values +/− standard deviation. *P<0.05, **P<0.005, ***P<0.001, n.s.=not significant. At least three independent experiments were performed.
γH2AX-intensity of Panc-1 and MIA PaCa-2 cells treated with irradiation and/or (A) oridonin (ORI) and (B) ponicidin (PON) as indicated. Results are presented as mean values +/− standard deviation. *P<0.05, **P<0.005, ***P<0.001, n.s.=not significant. At least three independent experiments were performed.

Figure 4

Western Blots of Panc-1 and MIA PaCa-2 cells treated with either (A) oridonin (ORI) and (B) ponicidin (PON) as indicated. One representative Western Blot is shown. At least two independent experiments were performed. NHEJ = non-homologous end-joining.
Western Blots of Panc-1 and MIA PaCa-2 cells treated with either (A) oridonin (ORI) and (B) ponicidin (PON) as indicated. One representative Western Blot is shown. At least two independent experiments were performed. NHEJ = non-homologous end-joining.
DOI: https://doi.org/10.1515/raon-2017-0048 | Journal eISSN: 1581-3207 | Journal ISSN: 1318-2099
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Language: English
Page range: 407 - 414
Submitted on: Jun 28, 2017
|
Accepted on: Oct 13, 2017
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Published on: Nov 29, 2017
Published by: Association of Radiology and Oncology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
oridonin,
ponicidin,
irradiation,
pancreatic cancer,
γH2AX,
radiosensitivity
Related subjects:
Medicine,
Clinical medicine,
Internal medicine,
Haematology, oncology,
Radiology

© 2017 Jakob Liermann, Patrick Naumann, Franco Fortunato, Thomas E. Schmid, Klaus-Josef Weber, Jürgen Debus, Stephanie E. Combs, published by Association of Radiology and Oncology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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