Have a personal or library account? Click to login
Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study Cover

Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study

Radiology and Oncology
Volume 51 (2017): Issue 3 (September 2017)
By: Claudia Salvadori,  Tanja Svara,  Guido Rocchigiani,  Francesca Millanta,  Darja Pavlin,  Maja Cemazar,  Ursa Lampreht Tratar,  Gregor Sersa,  Natasa Tozon and  Alessandro Poli  
Open Access
|Sep 2017

Figures & Tables

Figure 1

Histology of tissue samples collected before the combined therapy (T0) and at 4 (T1) and 8 weeks (T2) post-treatment. (A) At T0 sheets of neoplastic cells with abundant cytoplasm containing variable number of metachromatic granules were present in the superficial and deep dermis; (B) At T1 The neoplastic tissue was substituted by a fibrotic tissue associated with scanty inflammatory infiltrates mainly constituted by mononuclear cells; (C) At T2 in dogs with partial response between connective tissue bundles were evident thin aggregates of neoplastic mast cells. Haematoxylin Eosin; bar = 100 mm.
Histology of tissue samples collected before the combined therapy (T0) and at 4 (T1) and 8 weeks (T2) post-treatment. (A) At T0 sheets of neoplastic cells with abundant cytoplasm containing variable number of metachromatic granules were present in the superficial and deep dermis; (B) At T1 The neoplastic tissue was substituted by a fibrotic tissue associated with scanty inflammatory infiltrates mainly constituted by mononuclear cells; (C) At T2 in dogs with partial response between connective tissue bundles were evident thin aggregates of neoplastic mast cells. Haematoxylin Eosin; bar = 100 mm.

Figure 2

Immunoistochemical staining of tissue samples collected at T0 (A, C and E) and, T1 (B, D and E). CD3+ lymphocytes infiltrating the neoplastic tissue at T0 (A) and the fibrotic tissue at T1 (B). Scanty Foxp3+ Treg lymphocytes at the periphery of neoplastic tissue at T0 (C) and in a tissue sample collected at T1 (D). CD68+ macrophages in the neoplastic tissue at T0 (E) and in the fibrotic tissue at T1 (F). Immunohistochemical staining using DAB chromogen and haematoxylin counterstain. Bar = 100 mm.
Immunoistochemical staining of tissue samples collected at T0 (A, C and E) and, T1 (B, D and E). CD3+ lymphocytes infiltrating the neoplastic tissue at T0 (A) and the fibrotic tissue at T1 (B). Scanty Foxp3+ Treg lymphocytes at the periphery of neoplastic tissue at T0 (C) and in a tissue sample collected at T1 (D). CD68+ macrophages in the neoplastic tissue at T0 (E) and in the fibrotic tissue at T1 (F). Immunohistochemical staining using DAB chromogen and haematoxylin counterstain. Bar = 100 mm.

Figure 3

Histogram of number of immune cells in 10,000 µm2 of tissue samples collected al T0, T1 and T2. Slight increase of CD3+ lymphocytes at T1, while macrophages significantly increased at T2 and Treg lymphocytes at T1.
Histogram of number of immune cells in 10,000 µm2 of tissue samples collected al T0, T1 and T2. Slight increase of CD3+ lymphocytes at T1, while macrophages significantly increased at T2 and Treg lymphocytes at T1.

Figure 4

Immunoistochemical staining of tissue samples collected at T0(A, C and E) and , T1(B, D and E). Ki-67+ neoplastic cells at T0(A) and at T1 in a dog with partial response (B). Bcl-2+ neoplastic cells at T0(C) and at T1 in a dog with partial response (D). Microvessels stained using an anti-CD31 primary antibody at T0(E) and in the fibrotic tissue at T1 in a dog with a complete response (F). Immunohistochemical staining using DAB chromogen and haematoxylin counterstain. Bar = 100 mm.
Immunoistochemical staining of tissue samples collected at T0(A, C and E) and , T1(B, D and E). Ki-67+ neoplastic cells at T0(A) and at T1 in a dog with partial response (B). Bcl-2+ neoplastic cells at T0(C) and at T1 in a dog with partial response (D). Microvessels stained using an anti-CD31 primary antibody at T0(E) and in the fibrotic tissue at T1 in a dog with a complete response (F). Immunohistochemical staining using DAB chromogen and haematoxylin counterstain. Bar = 100 mm.

Figure 5

Histogram of microvessel density determineg using both primary antibodies against Factor VIII and CD31positive-cells in 50,000 µm2 of tissue samples and number of Ki-67+ and Bcl-2+ neoplastic cells in 10,000 µm2 of tissue samples collected al T0, T1 and T2. Microvessel density was drastically reduced at T1 and T2. as well as the proliferation activity of neoplastic cells, while Bcl-2 expression was increased at T1.
Histogram of microvessel density determineg using both primary antibodies against Factor VIII and CD31positive-cells in 50,000 µm2 of tissue samples and number of Ki-67+ and Bcl-2+ neoplastic cells in 10,000 µm2 of tissue samples collected al T0, T1 and T2. Microvessel density was drastically reduced at T1 and T2. as well as the proliferation activity of neoplastic cells, while Bcl-2 expression was increased at T1.

Antibodies used in the study

AntibodySpecificityTypeSpeciesSourceDilutionPretreatment
Anli-humun CD3Pan-T lymphocytesPoliconalRabbit(A0552) Dako UK Ltd. Ely UK1:50Citrate buffer pH6
Anti-human CD20Pan-B lymphocytesPoliconalRabbit(RB-9013-PO) Thermo Scientific, Chesire, UK1:400None
Anti-human Foxp3T-reg lymphocytesMonoclonalMouse(7979) Affymetrix eBioscience, san Diego, CA USA1:100Triss-EDTA pH9
Anti-human CD68MacrophagesMonoclonalMouse(PG-M1) Thermo Scientific, Chesire, UK1:100Proteinase K
Anti-human Ki-67Proliferating cellsMonoclonalMouse(610538) BD Biosciences, Wyckoff, NJ, USA51:100Citrate buffer pH6
Anti-human Bcl-2Antiapoptotic proteinMonoclonalMouse(610538) BD Biosciences, Wyckoff, NJ, USA51:100Citrate buffer pH6
Anti-human Von Willebrand Factor -Endothelial cellsPoliclonalRabbit(A0082) Dako UK Ltd. Ely UK1:300Citrate buffer pH6
Anti-human CD31Endothelial cellsMonoclonalMouse(JC70A) Dako UK Ltd. Ely UK1:100Citrate buffer pH6
DOI: https://doi.org/10.1515/raon-2017-0035 | Journal eISSN: 1581-3207 | Journal ISSN: 1318-2099
Journal RSS Feed
Language: English
Page range: 286 - 294
Submitted on: Jul 12, 2017
|
Accepted on: Aug 10, 2017
|
Published on: Sep 14, 2017
Published by: Association of Radiology and Oncology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
electrochemotherapy,
histopathology,
immune cells,
interleukin-12,
mast cell tumor,
microvessel density
Related subjects:
Medicine,
Clinical medicine,
Internal medicine,
Haematology, oncology,
Radiology

© 2017 Claudia Salvadori, Tanja Svara, Guido Rocchigiani, Francesca Millanta, Darja Pavlin, Maja Cemazar, Ursa Lampreht Tratar, Gregor Sersa, Natasa Tozon, Alessandro Poli, published by Association of Radiology and Oncology
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 51 (2017): Issue 3 (September 2017)Next article