Figure 1
Morphological characteristics of microalgae. D. salina isolate BAK (from Lake Bakhtegan) at 1M NaCl (a) and at 5M NaCl (b); and D. pseudosalina isolate MAH (from Lake Maharlu) at 1M NaCl (c) and at 5M NaCl (d). f – flagella and s – stigma
Figure 2
PCR products of 18S rDNA and ITS (~2500 bp) in D. pseudosalina isolate MAH and D. salina isolate BAK (a). RFLP pattern of PCR products presented in gel after digestion by Hhal (restriction enzyme) (b). PCR products of rbcL gene (~900 bp) in D. pseudosalina isolate MAH and D. salina isolate BAK (c). “Lan” 1 indicates the 18S rDNA PCR product of D. salina isolate BAK and “L” indicates Ladder
Table 1
The 18S rDNA sequences of Dunaliella spp. from the NCBI database
| Dunaliella spp. | Gene size (bp) | Accession number | Geographic origin |
|---|---|---|---|
| D. bardawil strain KMMCC 1346 | 2054 | JQ315779.1 | Republic of Korea |
| D. bardawil UTEX LB 2538 | 2088 | DQ009777.1 | USA |
| D. bioculata UTEX LB 199 | 1687 | DQ009761.1 | USA |
| D. parva | 2585 | M62998.1 | Unknown |
| D. peircei strain UTEX LB 2192 | 2065 | DQ009778.1 | USA |
| D. primolecta UTEX 1000 | 1620 | KJ018734.1 | USA |
| D. salina strain KMMCC 1428 | 1647 | JQ315781.1 | Korea |
| Dunaliella sp. ABRIINW M1/2 | 2120 | EU678868.1 | Iran |
| D. salina UTEX LB 200 | 2065 | DQ009779.1 | USA |
| D. salina strain KU07 | 2069 | KF825551.1 | Thailand |
| D. salina strain KU11 | 2067 | KF825550.1 | Thailand |
| D. salina strain KU13 | 2068 | KF825552.1 | Thailand |
| D. salina isolate BAK | 1784 | KU641617 | Iran |
| Dunaliella sp. SAS11133 | 1722 | KF054056.1 | China |
| D. pseudosalina isolate MAH | 1735 | KU641615 | Iran |
| D. salina isolate BAK | 1784 | KU641617.1 | Iran |
| Asteromonas gracilis isolate BA | 1687 | KU351659.1 | Iran |
| D. viridis strain CONC002 | 2494 | DQ009776.1 | USA |
| D. tertiolecta CCMP 364 | 1620 | KJ018735.1 | USA |
| D. tertiolecta UTEX 999 | 1620 | KJ018733.1 | USA |
| Chlamydomonas reinhardtii | 1641 | AB701555 | Japan |
| D. lateralis strain Nepal | 1692 | DQ009762.1 | USA |
| Chlorella vulgaris | 1770 | KJ756813 | UK |
| D. polymorpha | 2991 | KJ756825.1 | UK |
Table 2
ITS1 and ITS 2 sequences of Dunaliella spp. from the NCBI database
| Dunaliella spp. | ITS1 size | ITS2 size | Accession number | Geographic origin |
|---|---|---|---|---|
| D. bardawil strain KMMCC 1346 | 214 | 230 | JQ315779.1 | Republic of Korea |
| D. bardawil UTEX 2538 | 210 | 232 | DQ377085.1 | USA |
| D. biocolata strain UTEX 199 | 209 | 328 | DQ377086.1 | USA |
| D. parva | 213 | 226 | DQ116746 | China |
| D. peircei strain UTEX 2192 | 210 | 226 | AF313442.1 | Chile |
| D. primolecta UTEX 1000 | 210 | 328 | DQ377092.1 | USA |
| D. acidophila strain CCAP 19/35 | 213 | 232 | HM060646.1* | Spain |
| HM060645.1● | ||||
| D. salina strain KMMCC 1428 | 214 | 227 | JQ315781.1 | Republic of Korea |
| Dunaliella sp. ABRIINW M1/2 | 220 | 225 | EU927373.1 | Iran |
| D. Salina UTEX 200 | 209 | 227 | DQ313197.1 | Chile |
| D. salina strain KU07 | 159 | 229 | KF825555.2 | Thailand |
| D. salina strain KU11 | 159 | 229 | KF825549.1 | Thailand |
| D. salina strain KU13 | 159 | 229 | KF825547.1 | Thailand |
| D. salina isolate BAK | 216 | 233 | KU641617 | Iran |
| Dunaliella sp. SAS11133 | 204 | 230 | KF054058.1 | China |
| D. pseudosalina isolate MAH | 212 | 225 | KU641615 | Iran |
| Asteromonas gracilis isolate BA | 214 | 230 | KU351659.1 | Iran |
| D. viridis strain CONC002 | 217 | 228 | DQ377098.1 | USA |
| D. tertiolecta CCMP 364 | 210 | 228 | DQ377097.1 | USA |
| D. tertiolecta UTEX 999 | 210 | 226 | AF313434.1* | Chile |
| AF1313435.1● | ||||
| Chlamydomonas reinhardtii | 205 | 244 | U66954 | Japan |
| D. lateralis strain Nepal | 212 | 201 | DQ377089.1 | USA |
| Chlorella vulgaris | 291 | 404 | KJ756813 | UK |
| D. polymorpha | 213 | 227 | KJ756825 | UK |
Table 3
The rbcL sequences of Dunaliella spp. from the NCBI database
| Dunaliella spp. | rbcL size | Accession number | Geographic origin |
|---|---|---|---|
| Dunaliella bardawil strain KMMCC 1346 | 798 | JQ315489.1 | Republic of Korea |
| Dunaliella bardawil UTEX 2538 | 1038 | DQ313194.1 | USA |
| D. biocolata strain UTEX 199 | 1038 | DQ313195.1 | USA |
| D. parva | 1040 | DQ173091.1 | Chila |
| D. peircei strain UTEX LB 2192 | 869 | DQ313196.1 | USA |
| D. primolecta UTEX 1000 | 1038 | DQ313198.1 | USA |
| D. acidophila strain CCAP 19/35 | 667 | HQ142901.1 | Spain |
| D. salina strain KMMCC 1428 | 894 | JQ315491.1 | Korea |
| Dunaliella sp. ABRIINW M1/2 | 1320 | KC149893.1 | Iran |
| D. salina UTEX 200 | 869 | DQ313197.1 | USA |
| D. salina strain KU07 | 427 | KF825555.2 | Thailand |
| D. salina strain KU11 | 613 | KF825554.1 | Thailand |
| D. salina strain KU13 | 632 | KF825553.1 | Thailand |
| D. salina isolate BAK | 789 | KU682279 | Iran |
| Dunaliella sp. SAS11133 | 717 | KF054057.1 | China |
| D. pseudosalina isolate MAH | 799 | KU641616 | Iran |
| D. viridis strain CONC002 | 1038 | DQ313206.1 | USA |
| D. tertiolecta CCMP 364 | 1038 | DQ313204.1 | USA |
| D. tertiolecta UTEX 999 | 1038 | DQ313203.1 | USA |
| Chlamydomonas reinhardtii | 1128 | AB511846 | Japan |
| Chlorella vulgaris | 1428 | AB260909 | Japan |
| Asteromonas gracilis | 469 | JN033249.1 | Chile |
Figure 3
The 18S rDNA based phylogenetic tree. The tree was obtained by the ML method (K2 + G model) with 500 bootstrap replications. The capital letters (A–D) show the clades.
Figure 4
The ITS based phylogenetic tree. The tree was obtained by the ML method (K2 + G model) with 500 bootstrap replications. The capital letters (A–D) show the clades.
Figure 5
The rbcL based phylogenetic tree. The tree was obtained by the ML method (T92 + G model) with 500 bootstrap replications. The capital letters (A–D) indicate the clades.
Figure 6
Combined 18S rDNA, ITS and rbcL based phylogenetic tree. The tree was drawn using the ML method (TN93 + G model) with 500 bootstrap replications. The capital letters (A–D) indicate the clades.