Figure 1
Geographical location of the sampling areas: (•) St. Andrew and ACI Marina, Northern Adriatic, Croatia
Table 1
Environmental factors: temperature and salinity measured at St. Andrew (SA) and ACI Marina (AM) sites
| Month/ Environmental factor | Temperature (°C) | Salinity (PSU) | ||
|---|---|---|---|---|
| Site | SA | AM | SA | AM |
| September | 22.5 | 23.0 | 34.5 | 34.5 |
| October | 20.1 | 22.0 | 34.5 | 34.2 |
| November | 16.0 | 15.2 | 35.0 | 35.4 |
| December | 12.2 | 12.4 | 36.2 | 36.2 |
| January | 10.3 | 11.1 | 37.4 | 35.1 |
| February | 9.9 | 10.2 | 37.5 | 36.8 |
| March | 9.7 | 10.5 | 37.4 | 37.1 |
| April | 12.2 | 13.0 | 37.0 | 37.1 |
| May | 18.0 | 18.2 | 36.0 | 29.2 |
| June | 21.0 | 22.4 | 35.9 | 34.6 |
| July | 22.8 | 22.7 | 33.6 | 31.2 |
| August | 25.0 | 23.2 | 35.2 | 35.6 |
Figure 2
Light microscopy of transverse cryosections through the mussel Mytilus galloprovincialis digestive gland containing Nematopsis sp. A) One oocyst (oc) in connective tissue (ct) between digestive tubules (dt); B) host phagocyte (p) containing three oocysts (oc) in connective tissue (ct); C) oocysts located in a parasitophorous vacuole (pv) (lighter area). Note the oocyst wall (ocw) and the sporozoite (s) (scale bars = 10 μm)
Figure 3
Light microscopy of Urastoma cyprinae located in connective tissue (ct) at the edge of the digestive gland and digestive tubules (dt) (scale bar = 20 μm)
Figure 4
Light microscopy of transverse cryosections through the digestive gland of mussel Mytilus galloprovincialis containing conidia (c) of Alternaria sp. attached to epithelial cells of digestive tubules (dt) (scale bar = 20 μm)
Table 2
Prevalence (P) and intensity of infection (I) with Nematopsis sp., Urastoma cyprinae and Alternaria sp. (range given in parentheses) from Mytilus galloprovincialis mussels sampled at St. Andrew (SA) and ACI Marina (AM)
| Infection | Nematopsis sp. P (%) [I (range)] | U. cyprinae P (%) [I (range)] | Alternaria sp. P (%) [I (range)] | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Site | SA | AM | SA | AM | SA | AM | ||||||
| September | 100 | [12 (2-27)] | - | 20 | [1] | - | - | 20 | [1] | - | - | |
| October | 80 | [30 (12-38)] | 20 | [9 (9)] | - | - | - | - | - | - | - | - |
| November | 100 | [11 (6-17)] | - | - | - | - | - | 40 | [1] | - | - | |
| December | 60 | [4 (2-5)] | - | - | - | - | - | - | - | - | - | |
| January | 80 | [8 (1-17)] | 60 | [7(4-11)] | - | - | - | - | - | - | - | - |
| February | 60 | [6 (1-17)] | 40 | [6 (2-10)] | - | - | - | - | - | - | - | - |
| March | 80 | [9 (3-17)] | - | - | - | - | - | - | - | - | - | |
| April | 60 | [12 (9-17)] | 40 | [1(1)] | - | - | - | - | - | - | - | - |
| May | 60 | [10 (6-15)] | 40 | [3(2-3)] | - | - | - | - | - | - | - | - |
| June | 80 | [13 (2-26)] | - | - | - | - | - | - | - | - | - | |
| July | 40 | [74 (37-110)] | 40 | [3(1-4)] | - | - | - | - | - | - | - | - |
| August | 100 | [20 (2-60)] | 60 | [3(1-7)] | - | - | - | - | - | - | - | - |
[i] -not detected