
Figure 1
Location of the study area (a), location of the sampling sites at the Wołosaty and Terebowiec streams (b) and at the Wisłoka with its tributary and the Ryjak (c), the Wisłoka’s tributary (d); red dots represent sampling sites on the soil, blue dots at the watercourses
Table 1
Characteristics of the studied watercourses in Bieszczady and Magura National Park
| Rivers and streams | Number of sites | Watercourse length (km) | Short description |
|---|---|---|---|
| Bieszczady National Park | |||
| Wołosaty | 6 | 27.8 | right-bank tributary of the San River in the upper section called Wołosatka |
| Terebowiec | 2 | 8 | right-bank tributary of the Wołosaty |
| Rzeczyca | 1 | 13.2 | left-bank tributary of the Wołosaty |
| Magura National Park | |||
| Wisłoka | 6 | 164 | right-bank tributary of the Vistula |
| Wisłoka’s tributary | 1 | 0.9 | nameless, short right-bank tributary of the Wisłoka, drying during summer |
| Kłopotnica | 3 | 15 | left-bank tributary of the Wisłoka |
| Świerzówka | 1 | 8.3 | left-bank tributary of the Wisłoka |
| Rzeszówka | 1 | 8.2 | left-bank tributary of the Wisłoka |
| Ryjak | 3 | 12 | right-bank tributary of the Wisłoka |
| Ryjak’s tributary | 1 | 1.2 | nameless, short left-bank tributary of the Ryjak, |
| Krempna | 2 | 7.3 | right-bank tributary of the Wisłoka |
| Baranie | 2 | 5.8 | left-bank tributary of the Wilsznia |
| Zimna Woda | 2 | 4.8 | left-bank tributary of the Hucianka |
| Zimna Woda’s tributary | 1 | 0.8 | nameless, short right-bank tributary of the Zimna Woda |
| Wilsznia | 3 | 11.5 | right-bank tributary of the Wisłoka |

Figure 2
Luticola frequentisima LM images; population from the Wisłoka’s tributary (a-o), from the soil in the Wilsznia catchment (p-r) and from the Wołosaty and Terebowiec streams (z-ad)

Figure 3
Luticola frequentisima SEM images: population from the Wisłoka’s tributary (a-g), from the Ryjak’s tributary (h, i), from the soil in the Wilsznia catchment (j, k) and from the Wołosaty stream (l). Detailed external view of valve apex (f) and internal view of valve (g)
Table 2
Description of the sampling sites and valve dimensions of L. frequentissima
| Sampling site | Bieszczady National Park | Magura National Park | ||||
|---|---|---|---|---|---|---|
| Wołosaty | Terebowiec | Ryjak’s tributary | Wisłoka | Wisłoka’s tributary | soil (Ryjak, Wilsznia catchments) | |
| Date | 03/2014 | 03/2014 | 07/2013 | 10/2013 | 04/2013 10/2014 | 04/2014 07/2014 10/2014 |
| Insolation | high | low | low | high | low | high |
| Type of substrate | stones (large ones covered with moss) | stones | stones (near shore covered with mud) | stones | stones (large ones covered with moss) | soil overgrown with grass in ca. 50% |
| Temperature (°C) | 5.6 | 6.1 | 13.4 | 10.2-11.7 | 8.1-11.8 | - |
| pH | 8.5 | 8.4 | 8.1 | 6.3-6.5 | 7.3-7.4 | 5.6-6.5 |
| Conductivity (μS cm–1) | 166 | 138 | 288 | 378-402 | 174-341 | - |
| NO3- (mg l-1) | 0.22 | 0.23 | 0.84 | <0.01 | 1.91-5.76 | - |
| SO42- (mg l-1) | 16.37 | 13.74 | 18.78 | 20.39-26.53 | 18.20-21.26 | - |
| L. frequentissima valve dimensions | ||||||
| Length (μm) | 9.4 | 14.1 | 8-13.3 | 11.6-16.5 | 6.2-22.5 | 8.6-16.6 |
| Breadth (μm) | 5.0 | 6.6 | 5.5-6.2 | 4.0-6.0 | 4.4-7.6 | 5.1-6.4 |
| Striae per 10 μm | 22 | 21 | 23-27 | 22 | 18-21 | 22-27 |