
Figure 1
Map of sampling sites on the Czarna Staszowska River
Table 1
Values of physical and chemical water parameters in the spring section of the Czarna Staszowska River from 2014 and the water quality class according to Polish standards compatible with OJ pos. 1482 from 2014
| Water parameters | Spring section (sites 1, 2) | Water Quality Class |
|---|---|---|
| Temperature (°C) | 10.5 | I |
| pH | 7.6-7.9 | I |
| Conductivity 20°C (µS cm 1) | 405 | I |
| Dissolved oxygen (DO) (mg O2 dm−3) | 10.0 | I |
| BOD5 (mg O2 dm−3) | 1.9 | I |
| N-NH4 (mg dm−3) | 0.23 | I |
| N-NO3 (mg dm−3) | 2.0 | I |
| Total N (mg dm−3) | 2.783 | I |
| P-PO4 (mg dm−3) | 0.1 | I |
| Total P (mg P dm−3) | 0.13 | I |
| Calcium (mg Ca dm−3) | 64 | I |
| Magnesium (mg Mg dm−3) | 10 | I |
| Total hardness (mg CaCO3 dm−3) | 202 | I |

Figure 2
LM photomicrographs of Prestauroneis tumida Levkov (1-4); SEM images of external view (5-7); SEM image of internal view (8)
Table 2
Comparison of morphological features of species similar to Prestauroneis tumida, in chronological order
| Length | Width | Number of striae in 10 μm | Number of areolae in 10 μm | Plate/Figure | |
|---|---|---|---|---|---|
| Hustedt (1930) Navicula crucicula var. obtusata Grunow | 25-50 | 10-16 | - | - | - |
| Cleve-Euler (1953) Navicula crucicula β obtusata Grunow Navicula crucicula var. minor Agardh Cleve | 25-50 30-50 | 10-16 10-13 | 19 - | - - | Fig. 884 c d |
| Germain (1981) Navicula protracta (Grunow) Cleve | 30-35 | 8-10 | 14-17 | - | Plate 83, Fig. 6 |
| Krammer & Lange Bertalot (1986) Navicula crucicula var. crucicula (W. Smith) Donkin Navicula protracta (Grunow) Cleve | 35-100 17-60 | 8-23 5-10 | 14-18 14-20 | - - | Fig. 54: 1 Fig. 55: 5, 6 |
| Simonsen (1987) Navicula protracta f. elliptica Hustedt | - | - | - | - | Plate 659, Fig. 1, 2 |
| Levkov et al. (2007) Parlibellus protractus (Grunow) Witkowski, Lange-Bertalot & Metzeltin | 32-44 | 9-11 | 14-16 | - | Plate 64, Fig. 12 |
| Levkov & Williams (2011) Prestauroneis tumida Levkov | 22-44 | 9-11 | 14-17 | 45 | Fig. 69-82 |

Figure 3
Comparison of valve morphology of Prestauroneis tumida and Parlibellus protracta. A. LM photomicrograph of Prestauroneis tumida; Arrow 1: rounded apices; Arrow 2: pseudosepta on apices. B. LM photomicrograph of Parlibellus protracta; Arrow: capitate apices. C. SEM image of Prestauroneis tumida, internal view; Arrow: presence of pseudosepta. D. SEM image of Parlibellus protracta, internal view: pseudosepta absent