Contracaecum spp. from endemic Baikal fishes: the Baikal yellowfin Cottocomephorus grewingkii (Dybowski, 1874) and the longfin Baikal sculpin Cottocomephorus inermis (Yakovlev, 1890)

Rusinek, Olga; Kulikowski, Marek; Najda, Katarzyna; Rokicki, Jerzy

doi:10.1515/ohs-2015-0007

Abstract

All the nematodes found in body cavities of the examined endemic Baikal fishes: 88 Baikal yellowfin Cottocomephorus grewingkii (Dybowski, 1874) and 35 longfin Baikal sculpin Cottocomephorus inermis (Yakovlev, 1890) were identified as Contracaecum osculatum baicalensis (Mozgovoi and Ryjikov, 1950) L3 larvae. The prevalence, mean intensity, intensity range and abundance of the nematodes in C. grewingkii were 37.5%, 2.55, 1-31, and 0.96, respectively, the corresponding values in C. inermis were 60.0%, 2.43, 1-10, and 1.46. The infestation level in C. grewingkii was significantly higher than in C. inermis (Mann-Whitney U-test, p<0.02). The number of parasites was found to increase with the fish length. Although in both and C. inermis, the anisakids were more frequent in males (prevalence of 52.17 and 67.76%, respectively) than in females (prevalence of 35.39 and 42.86%, respectively), differences between the sexes in the infestation level in the two species were not significant (Mann-Whitney U-test, P= 0.09 and P=0.23, respectively). The molecular method applied (PCF-RFLP) allows to identify all the nematodes in both examined fish species as C. osculatum baicalensis.

References

Bauer, O.N. (1987). Key to the parasites of freshwater fish of the USSR. Metazoan parasites. Leningrad: Izdatel’stvo Nauka (in Russian).
Search in Google Scholar
D’Amelio, S., Mattiucci, S., Paggi, L., Koie, L., Podvyaznaya, I., Pugachev, O., Rusinek, O., Timoshkin, O. & Nascetti, G. (1995). Taxonomic rank and origin of Contracaecum osculatum baicalensis Mozgovoi and Ryjikov, 1950, parasite of Phoca sibirica from Lake Baikal, with data on its occurrence in fish hosts. Abstracts of 4th International Symposium of Fish Parasitology, Munich October 3-7, 27.
Search in Google Scholar
Dogel’, V.A., Bogolepova, I.I. & Smirnova, K.V. (1949). Parazitofauna Bajkala i eë zoogeografičeskoe značenie, Vestnik Leningradskogo Universiteta 7: 13-34 (in Russian).
Search in Google Scholar
Dzido, J. (2011). Identyfikacja molekularna oraz zrożnicowanie wewnątrzgatunkowe pasożytniczych nicieni z rodziny Anisakidae. Unpublished doctoral dissertation, University of Gdańsk, Gdańsk, Poland.
Search in Google Scholar
Dzyuba, E.V. (2004). Issledowanie piŝčevyh strategii pelagičeskich ryb Bajkala. Unpublished doctoral dissertation, Institut Biologii Vnutrennyh Vod im. I.D. Papanina RAN, Borok, Russia (in Russian).
Search in Google Scholar
Dzyuba, E.V., Mel’nik, N.G. & Naumova, E.Y. (2000). Feeding spectra of the young of Cottocomephorus inermis (Cottidae) in Lake Baikal. J. Ichthyol. 40: 342-345.
Search in Google Scholar
Hoarau, G., Holla, S., Lescasse, R., Stam, W.T. & Olsen, J.L. (2002). Heteroplasmy and evidence for recombination in the mitochondrial control region of the flatfish Platichthys flesus. Mol. Biol. Evol. 19(12): 2261-2264.
Search in Google Scholar
Kijewska, A., Rokicki, J., Sitko, J. & Węgrzyn, G. (2002). Ascaridoidea: A simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds. Exp. Parasitol. 101(1): 35-39. DOI: 10.1016/S0014-4894(02)00031-0.10.1016/S0014-4894(02)00031-0
Search in Google Scholar
Koie, M. & Fagerholm, H. P. (1995). The life cycle of Contracaecum osculatum (Rudolphi, 1802) sensu stricto (Nematoda, Ascaridoidea, Anisakidae) in view of experimental infections. Parasitol. Res. 81: 481-489.
Search in Google Scholar
Kozhov, M.M. (1963). Lake Baikal and its Life. Monogr. Biol. 11, The Hague: Junk Publishers.10.1007/978-94-015-7388-7
Search in Google Scholar
Lâjman, E.M. (1933). Parazitičeskie červi ozera Bajkal. Trudy Bajkalskoj Limnologičeskoj Stancji 4: 5-98 (in Russian).
Search in Google Scholar
Margolis, L., Esch, G. W., Holmes, J. C., Kuris, A. M. & Schad G. A. (1982). The use of ecological terms in parasitology (report of an ad hoc committee of the American Society of Parasitologists), J. Parasitol. 68(1): 131 - 133.
Search in Google Scholar
Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D’Amelio, S., Orecchia, P. & Bullini, L. (1997). Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). J. Parasitol. 83(3): 401-416.
Search in Google Scholar
Mattiucci, S. & Nascetti, G. (2007). Genetic diversity and infection levels of anisakid nematodes parasitic in fish and marine mammals from Boreal and Austral hemispheres. Vet. Parasitol. 148: 43-57. DOI: 10.1016/j.vetpar.2007.05.009.10.1016/j.vetpar.2007.05.009
Search in Google Scholar
Mattiucci, S. & Nascetti, G. (2008). Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host - parasite co - evolutionary processes. Adv. Parasitol. 66: 48 - 148. DOI: 10.1016/S0065-308X(08)00202-9.10.1016/S0065-308X(08)00202-9
Search in Google Scholar
Mazepova, G.F. (1998). The role of copepods in the Baikal ecosystem. J. Mar. Syst. 15: 113-120. DOI: 10.1016/S0924-7963(97)00065-1.10.1016/S0924-7963(97)00065-1
Search in Google Scholar
Melnik, N.G., Timoshkin, O.A. & Sideleva, V.G. (1995). Distribution of M. branickii and some characteristics of its ecology. Guide and key to pelagic animals of Baikal. Novosibirsk: Izdatel’stvo Nauka.
Search in Google Scholar
Rolbiecki, L. (2002). A rapid method for preparing semipermanent glycerol-jelly parasite Mount. Wiadomości Parazytologiczne 48: 87-88.
Search in Google Scholar
Rudstam, L.G., Melnik, N.G., Timoshkin, O.A., Hansson, S., Pushkin, S.V. & Nemov, V. (1992). Diel Dynamics of an Aggregation of Macrohectopus Branickii (DYB.) (Amphipoda, Gammaridae) in the Barguzin Bay, Lake Baikal, Russia. J. Gt. Lakes Res. 18(2): 286-297. DOI: 10.1016/S0380-1330(92)71296-9.10.1016/S0380-1330(92)71296-9
Search in Google Scholar
Rusinek, O.T. (2007). Fish parasites of Lake Baikal (fauna, communities, zoogeography and historical background). Moscow: KMK Scientific Press Ltd. (in Russian).
Search in Google Scholar
Sambrook, J., Fritsh, E.F. & Maniatis, T. (1989). Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Laboratory Press.
Search in Google Scholar
Sudarikov, V.E. & Ryzhikov, K.M. (1951). K biologii Contracaecum osculatum baicalensis - nematody bajkalskoj nerpy. Trudy Gel’mintologičeskoj Laboratorii 55: 59-66, (in Russian).
Search in Google Scholar
Valtonen, E.T., Fagerholm, H.P. & Helle, E. (1988). Contracaecum osculatum (Nematoda: Anisakidae) in fish and seals from Bothnian Bay (Northeastern Baltic Sea). Int. J. Parasitol. 18(3): 365-370. DOI: 10.1016/0020-7519(88)90146-4.10.1016/0020-7519(88)90146-4
Search in Google Scholar
Zaika V.E. (1965). Parazitofauna ryb ozera Bajkal. Russia: Izdatel’stvo Nauka, (in Russian).
Search in Google Scholar
Zhu, X., D’Amelio, S., Paggi, L. & Gasser, R.B. (2000). Assessing sequence variation in internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitol. Res. 86(8): 677 - 683. DOI: 10.1007/PL00008551.10.1007/PL00008551
Search in Google Scholar
Zhu, X., Gasser, R.B., Podolska, M. & Chilton, N.B. (1998). Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int. J. Parasitol. 28(12): 1911 - 1921. DOI: 10.1016/S0020-7519(98)00150-7.10.1016/S0020-7519(98)00150-7
Search in Google Scholar
Zubin, A.A. (1992). Diet of benthopelagic Baikal sculpins (Scorpaeniformes, Cottoidei), J. Ichthyol. 32: 42-47.
Search in Google Scholar

Contracaecum spp. from endemic Baikal fishes: the Baikal yellowfin Cottocomephorus grewingkii (Dybowski, 1874) and the longfin Baikal sculpin Cottocomephorus inermis (Yakovlev, 1890)

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