MDA and GSH-Px activity in transition dairy cows under seasonal variations and their relationship with reproductive performance

Colakoglu, Hatice Esra; Yazlik, Murat Onur; Kaya, Ufuk; Colakoglu, Ekrem Cagatay; Kurt, Serdal; Oz, Burcu; Bayramoglu, Rustem; Vural, Mehmet Rifat; Kuplulu, Sukru

doi:10.1515/jvetres-2017-0067

Abstract

Introduction: The purpose of the current study was to evaluate the blood glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels under seasonal variations in dairy cows during transition period, and to assess the relationship between chosen reproductive parameters, GSH-Px, and MDA.

Material and Methods: Holstein cows calving in January were assigned into winter group (n = 42), while cows calving in August were assigned into summer group (n = 42). Blood samples were collected from the jugular vein 21, 14, and 7 days before calving, at calving (0 day), and 7, 14, and 21 days after calving. Reproductive parameters obtained from farm records were evaluated.

Results: In both groups of cows, GSH-Px activity decreased from 21 days before calving to day 0, and it gradually continued to increase until 21 days after calving. GSH-Px activity was higher in winter group compared to summer group during the transition period (P < 0.05). MDA levels in both groups increased over time starting from 21 days before calving to 0 day, but it gradually decreased thereafter. MDA levels were higher in summer group compared to winter group during the transition periods (P < 0.05). Summer group of cows showed higher intervals of calving-to-oestrus, calving-to-conception, and higher insemination index (P < 0.01). Negative correlation was recorded between GSH-Px and MDA during all examination days (P < 0.01). MDA levels correlated with calving to conception interval on day 21 before calving and day 0 (P < 0.01) and insemination index on day 0 and 21 days after calving (P < 0.01). GSH-Px activity was negatively correlated with calving to conception interval on day 21 before calving, day 0, and 21 days (P < 0.01) after calving. Negative correlation on day 21 before calving and day 0 was also determined between GSH-Px and insemination index (P < 0.01).

Conclusion: This study showed that blood oxidant and antioxidant levels have affected the fertility parameters in cows under seasonal variations.

References

1. Abd Ellah M.R.: Oxidant and antioxidants during the transition period in dairy cows. J Adv Vet Res 2016, 6, 130–133.
Search in Google Scholar Back to article
2. Aitken S.L., Karcher E.L., Rezamand P., Gandy J.C., VandeHaar M.J., Capuco A.V., Sordillo L.M.: Evaluation of antioxidant and proinflammatory gene expression in bovine mammary tissue during the periparturient period. J Dairy Sci 2009, 92, 589–598.10.3168/jds.2008-1551
Open DOI Search in Google Scholar Back to article
3. Alila-Johansson A., Eriksson L., Soveri T., Laakso M.L.: Serum cortisol levels in goats exhibit seasonal but not daily rhythmicity. Chronobiol Int 2003, 20, 65–79.10.1081/CBI-120017684
Search in Google Scholar Back to article
4. Appasamy M., Jauniaux E., Serhal, P., Al-Qahtani A., Groome N.P., Muttukrishna S.: Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation. Fertil Steril 2008, 89, 912–921.10.1016/j.fertnstert.2007.04.034
Search in Google Scholar Back to article
5. Bernabucci U., Ronchi B., Lacetera N., Nardone A.: Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J Dairy Sci 2005, 88, 2017–2026.10.3168/jds.S0022-0302(05)72878-2
Open DOI Search in Google Scholar Back to article
6. Bernabucci U., Ronchi B., Lacetera N., Nardone A.: Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. J Dairy Sci 2002, 85, 2173–2139.10.3168/jds.S0022-0302(02)74296-3
Search in Google Scholar Back to article
7. Bhat S., Rao G., Murthy D.K., Bhat P.G.: Seasonal variations in markers of stress and oxidative stress in rats. Indian J Clin Biochem 2008, 23, 191–194.10.1007/s12291-008-0042-2345309123105750
Open DOI Search in Google Scholar Back to article
8. Castillo C., Hernandez J., Bravo A., Lopez-Alonso M., Benedito J.L.: Oxidative status during late pregnancy and early lactation in dairy cows. Vet J 2005, 169, 286–292.10.1016/j.tvjl.2004.02.00115727923
Search in Google Scholar Back to article
9. Castillo C., Hernandez J., Valverde I., Pereira V., Sotillo J., Alonso M.L., Benedito J.L.: Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Res Vet Sci 2006, 80, 133–139.10.1016/j.rvsc.2005.06.00316084546
Open DOI Search in Google Scholar Back to article
10. Cigliano L., Strazzullo M., Rossetti C., Grazioli G., Auriemma G., Sarubbi F., Iannuzzi C., Iannuzzi L., Spagnuolo M.S.: Characterization of blood redox status of early and mid-late lactating dairy cows. Czech J Anim Sci 2014, 59, 170–181.10.17221/7341-CJAS
Search in Google Scholar Back to article
11. Daley C.A., Macfarlane M.S., Sakurai H., Adams T.E.: Effect of stress-like concentrations of cortisol on follicular development and the preovulatory surge of LH in sheep. J Reprod Fertil 1999, 117, 11–16.10.1530/jrf.0.117001110645240
Search in Google Scholar Back to article
12. Das R., Sailo L., Verma N., Bharti P., Saikia J., Imtiwati, Kumar R.: Impact of heat stress on health and performance of dairy animals: A review. Vet World 2016, 9, 260–268.10.14202/vetworld.2016.260-268
Search in Google Scholar Back to article
13. Dash S., Chakravarty A.K., Singh A., Upadhyay A., Singh M., Yousuf S.: Effect of heat stress on reproductive performances of dairy cattle and buffaloes: A review. Vet World 2016, 9, 235–244.10.14202/vetworld.2016.235-244
Search in Google Scholar Back to article
14. De Rensis F., Lopez-Gatius F., García-Ispierto I., Morini G., Scaramuzzi R.J.: Causes of declining fertility in dairy cows during the warm season. Theriogenology 2017, 15, 145–153.10.1016/j.theriogenology.2016.12.024
Open DOI Search in Google Scholar Back to article
15. De Rensis F., Scaramuzzi R.J.: Heat stress and seasonal effects on reproduction in the dairy cow – a review. Theriogenology 2003, 60, 1139–1151.10.1016/S0093-691X(03)00126-2
Open DOI Search in Google Scholar Back to article
16. Festila I., Miresan V., Raducu C., Cocan D., Constantinescu R., Coroian A.: Evaluation of oxidative stress in dairy cow through antioxidant enzymes glutathione peroxidase (GPX) and superoxide dismutase (SOD). Bulletin UASVM Anim Sci Biotechnol 2012, 69, 107–110.
Search in Google Scholar Back to article
17. Fox P.F., Kelly A.L.: Indigenous enzymes in milk, overview and historical aspects – part 2. Int Dairy J 2006, 16, 517–532.10.1016/j.idairyj.2005.09.017
Open DOI Search in Google Scholar Back to article
18. Gong J., Xiao M.: Selenium and antioxidant status in dairy cows at different stages of lactation. Biol Trace Elem Res. 2016, 171, 89–93.
Search in Google Scholar Back to article
19. Hafeman D.G., Sunde R.A., Hoekstra W.G.: Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr 1974, 104, 580–587.10.1093/jn/104.5.5804823943
Open DOI Search in Google Scholar Back to article
20. Halliwell B., Gutteridge J.M.C.: Free radicals in biology and medicine. Oxford University Press. Grune Strottan, New York, 2007.
Search in Google Scholar Back to article
21. Komesaroff P.A., Esler M., Clarke I.J., Fullerton M.J., Funder J.W.: Effects of estrogen and estrous cycle on glucocorticoid and catecholamine responses to stress in sheep. Am J Physiol 1998, 275, 671–678.10.1152/ajpendo.1998.275.4.E6719755087
Search in Google Scholar Back to article
22. Konvicna J., Vargova M., Pulikova I., Kovac G., Kostecka Z.: Oxidative stress and antioxidant status in dairy cows during prepartal and postpartal periods. Acta Vet Brno 2015, 84, 133–140.10.2754/avb201584020133
Search in Google Scholar Back to article
23. Kumar J., Madan A.M., Kumar M., Sirohi R., Yadav B., Reddy A.V., Swain D.K.: Impact of season on antioxidants, nuritional metabolic status, cortisol and heat shock proteins in Hariana and Sahiwal cattle. Biol Rhythm Res 2017, doi:10.1080/09291016.2017.1332842.10.1080/09291016.2017.1332842
Open DOI Search in Google Scholar Back to article
24. Kurokawa Y., Yamashita R., Okita M., Yoshitoshi R., Sugino T., Obitsu T., Kawamura K.: A comparision of plasma glucose and oxidative status in lactating dairy cows in summer and autumn. Anim Sci J 2016, 87, 1212–1217.10.1111/asj.1254826800245
Search in Google Scholar Back to article
25. Leblanc S.J.: Monitoring metabolic health of dairy cattle in the transition period. J Reprod Dev 2010, 56, 29–35.10.1262/jrd.1056S29
Search in Google Scholar Back to article
26. Lutosławska G., Tkaczyk J., Panczenko-Kresowska B., Hubner-Wozniak E., Skierska E., Gajewski A.K.: Plasma TBARS, blood GSH concentrations, and erythrocyte antioxidant enzyme activities in regularly menstruating women with ovulatory and anovulatory menstrual cycles. Clin Chim Acta 2003, 331, 159–163.10.1016/S0009-8981(03)00085-8
Search in Google Scholar Back to article
27. Maibam U., Hooda O.M., Sharma P.S., Singh S.V., Upadhyay R.C.: Seasonal change in oxidative stress markers in blood plasma of Tharparkar (Bos indicus) and Karan Fries (Bos indicus x Bos taurus) cattle under tropical climate. Int J Curr Microbiol App Sci 2017, 6, 1720–1730.10.20546/ijcmas.2017.602.192
Open DOI Search in Google Scholar Back to article
28. Megahed G.A., Anwar M.M., Wasfy S.I., Hammadeh M.E.: Influence of heat stress on the cortisol and oxidant-antioxidants balance during oestrous phase in buffalo-cows (Bubalus bubalis): Thermo-protective role of antioxidant treatment. Reprod Dom Anim 2008, 43, 672–677.10.1111/j.1439-0531.2007.00968.x18673331
Search in Google Scholar Back to article
29. Mudron P., Konvicna J.: Thiobarbituric acid reactive substances and plasma antioxidative capacity in dairy cows at different lactation stages. Dtsch Tierarztl Wochenschr 2006, 113, 189–191.
Search in Google Scholar Back to article
30. Omidi A., Fathi M.H., Parker M.O.: Alterations of antioxidant status in dairy cows during lactation and in the dry period. J Dairy Res 2017, 84, 49–53.10.1017/S002202991600075328007040
Open DOI Search in Google Scholar Back to article
31. Orzechowski A., Ostaszewski P., Brodnicka A., Wilczak J., Jank M., Balasinska B., Grzelkowska K., Ploszaj T., Olczak J., Mrowczynska A.: Excess of glucocorticoids impairs wholebody antioxidant status in young rats. Relation to the effect of dexamethasone in soleus muscle and spleen. Horm Metab Res. 2000, 32, 174–181.10.1055/s-2007-97861710871157
Open DOI Search in Google Scholar Back to article
32. Ozkaya M.O., Nazıroglu M.: Multivitamin and mineral supplementation modulates oxidative stress and antioxidant vitamin levels in serum and follicular fluid of women undergoing in vitro fertilization. Fertil Steril 2010, 94, 2465–2460.10.1016/j.fertnstert.2010.01.06620226443
Open DOI Search in Google Scholar Back to article
33. Pilarczyk B., Jankowiak D., Tomza-Marciniak A., Pilarczyk R., Sablik P., Drozd R., Tylkowska A., Skolmowska M.: Selenium concentration and glutathione peroxidase (GSH-Px) activity in serum of cows at different stages of lactation. Biol Trace Elem Res 2012, 147, 91–96.10.1007/s12011-011-9271-y22089971
Search in Google Scholar Back to article
34. Rizzo A., Mutinati M., Spedicato M., Minoia G., Trisolini C., Jirillo F., Sciorsci R.L.: First demonstration of an increased serum level of reactive oxygen species during the peripartal period in the ewes. Immunopharmacol Immunotoxicol 2008, 30, 741–746.10.1080/0892397080227905018975384
Search in Google Scholar Back to article
35. Sakatani M., Balboula A.Z., Yamanaka K., Takahashi M.: Effect of summer heat enviroment on body temperature, estrous cycles, and blood antioxidant levels in Japanese Black cow. Anim Sci J 2012, 83, 394–402.10.1111/j.1740-0929.2011.00967.x22574791
Open DOI Search in Google Scholar Back to article
36. Saleh M., Salam A., Mileegy I.H.M.: Oxidative antioxidant status during transition from late pregnancy to early lactation in native and cross bred cows in the Egyptian oasis. Assiut Vet Med J 2007, 53, 113.10.21608/avmj.2007.251500
Search in Google Scholar Back to article
37. Sharma N., Singh N.K., Singh O.P., Pandey V., Verma P.K.: Oxidative stress and antioxidant status during transition period in dairy cows. Asian-Aust. J Anim Sci 2011, 24, 479–484.10.5713/ajas.2011.10220
Search in Google Scholar Back to article
38. Simonov M., Petruh I., Vlizlo V.: Processes of lipid peroxidation and antioxidant defense in dairy cows depending on lactation period and season. Rocz Nauk Zoot 2015, 42, 107–115.
Search in Google Scholar Back to article
39. Sordillo L.M., Aitken S.L.: Impact of oxidative stress on the health and immune function of dairy cattle. Vet Immunol Immunopathol 2009, 128, 104–109.10.1016/j.vetimm.2008.10.305
Search in Google Scholar Back to article
40. Sundrum A.: Metabolic disorders in the transition period indicate that the dairy cows’ ability to adapt is overstressed. Animals 2015, 5, 978–1020.10.3390/ani5040395
Search in Google Scholar Back to article
41. Sunil Kumar B.V., Kumar A., Meena K.: Effect of heat stress in tropical livestock and different strategies for its amelioration. J Stress Physiol Biochem 2011, 7, 45–54.
Search in Google Scholar Back to article
42. Turk R., Podpecan O., Mrkun J., Flegar-Mestric Z., Perkov S., Zrimsek P.: The effect of seasonal thermal stress on lipid mobilisation, antioxidant status and reproductive performance in dairy cows. Reprod Dom Anim 2015, 50, 595–603.10.1111/rda.12534
Search in Google Scholar Back to article
43. Uchiyama M., Mihara M.: Determiantion of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978, 86, 271–278.10.1016/0003-2697(78)90342-1
Open DOI Search in Google Scholar Back to article
44. Wolfenson D., Sonego H., Bloch A., Shaham-Albalancy A., Kaim M., Folman Y., Meidan R.: Seasonal differences in progesterone production by luteinized bovine thecal and granulosa cells. Domest Anim Endocrinol 2002, 22, 81–90.10.1016/S0739-7240(01)00127-8
Search in Google Scholar Back to article

MDA and GSH-Px activity in transition dairy cows under seasonal variations and their relationship with reproductive performance

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