Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

Tomasz Śniegocki; Małgorzata Gbylik-Sikorska; Andrzej Posyniak

doi:10.1515/jvetres-2017-0032

Abstract

Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.

References

1. Berendsen B.J., Zuidema T., de Jong J., Stolker L.A., Nielen M.W.: Discrimination of eight chloramphenicol isomers by liquid chromatography tandem mass spectrometry in order to investigate the natural occurrence of chloramphenicol. Anal Chim Acta 2011, 700, 78-85.10.1016/j.aca.2010.11.009
Search in Google Scholar Back to article
2. Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. OJ L 221, 17.8.2002, 8-36.
Search in Google Scholar Back to article
3. Guy P.A., Royer D., Mottier P., Gremaud E., Perisset A., Stadler R.H.: Quantitative determination of chloramphenicol in milk powders by isotope dilution liquid chromatography coupled to tandem mass spectrometry. J Chromatogr A 2004, 1054, 365-371.10.1016/S0021-9673(04)01290-7
Search in Google Scholar Back to article
4. Kittler K., Radeck W., Polzer J.: Investigations on the influence of hydrolysis on the total amount of marker residue and consequences. Conference Material, EuroResidue VIII, in Egmond aan Zee, the Netherlands 2016. pp. 129-131.
Search in Google Scholar Back to article
5. Nicolich R.S., Werneck-Barroso E., Marques M.A.S.: Food safety evaluation: Detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry. Anal Chim Acta 2006, 565, 97-102.10.1016/j.aca.2006.01.105
Search in Google Scholar Back to article
6. RASFF - the Rapid Alert System for Food and Feed. https://webgate.ec.europa.eu/rasff-window/portal/?event=searchResultList.
Search in Google Scholar Back to article
7. Rejtharová M., Rejthar L.: Determination of chloramphenicol in urine, feed water, milk, and honey samples using molecular imprinted polymer clean-up. J Chromatogr A 2009, 1216, 8246-8253.10.1016/j.chroma.2009.07.03719647262
Search in Google Scholar Back to article
8. Rezende D.R., Filho N.F., Rocha G.L. Simultaneous determination of chloramphenicol and florfenicol in liquid milk, milk powder, and bovine muscles by LC-MS/MS. Food Addit Contam A 2012, 29, 559-570.10.1080/19440049.2011.64116122239562
Search in Google Scholar Back to article
9. Rocha Siqueira S.R., Luiz Donato J., de Nucci G., Reyes, F.G.R.: A high-throughput method for determining chloramphenicol residues in poultry, egg, shrimp, fish, swine, and bovine using LC-ESI-MS/MS. J Sep Sci 2009, 32, 4012-4019.10.1002/jssc.20090034519937962
Open DOI Search in Google Scholar Back to article
10. Rønning H.T., Einarsen K., Asp T.N.: Determination of chloramphenicol residues in meat, seafood, egg, honey, milk, plasma, and urine with liquid chromatography-tandem mass spectrometry, and the validation of the method based on 2002/657/EC. J Chromatogr A 2006, 1118, 226-233.10.1016/j.chroma.2006.03.09916631764
Search in Google Scholar Back to article
11. Shen J., Xia X., Jiang H., Li C., Li J., Li X., Ding S.: Determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry andporcine muscle and liver by gas chromatography-negative chemical ionization mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2009, 877, 1523-1529.10.1016/j.jchromb.2009.03.04019395324
Search in Google Scholar Back to article
12. Sniegocki T., Posyniak A., Gbylik-Sikorska M., Zmudzki J.: Determination of chloramphenicol in milk using a QuEChERSbased on liquid chromatography tandem mass spectrometry Method Anal Letters 2014, 47, 568-578.10.1080/00032719.2013.848638
Search in Google Scholar Back to article
13. Sniegocki T., Posyniak A., Zmudzki J.: Determination of chloramphenicol residues in milk by gas and liquid chromatography mass spectrometry methods. Bull Vet Inst Pulawy 2007, 51, 59-64.
Search in Google Scholar Back to article
14. Sniegocki T., Gbylik-Sikorska M., Posyniak A.: Transfer of chloramphenicol from milk to commercial dairy products - experimental proof. Food Control 2015, 57, 411-418. 10.1016/j.foodcont.2015.04.028
Search in Google Scholar Back to article
15. Tajik H., Malekinejad H., Razavi-Rouhani S.M., Pajouhi M.R., Mahmoudi R., Haghnazari A.: Chloramphenicol residues in chicken liver, kidney and muscle: a comparison among the antibacterial residues monitoring methods of Four Plate Test, ELISA and HPLC. Food Chem Toxicol 2010, 48, 2464-2468.10.1016/j.fct.2010.06.01420600543
Open DOI Search in Google Scholar Back to article
16. Vivekanandan K., Swamy M. G., Prasad S., Mukherjee R.: A simple method of isolation of chloramphenicol in honey and its estimation by liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 2005, 19, 3025-3030.10.1002/rcm.216916193535
Search in Google Scholar Back to article

Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

Abstract

Paradigm

My account