Skip to main content
Have a personal or library account? Click to login
Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China Cover

Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China

Open Access
|May 2016

References

  1. 1. Aryam M., Shergill I.S., Williamson M., Gommersall L., Arya N., Patel H.: Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn 2005, 5, 209–219.
  2. 2. Carvajal I.L., Rafael D., Pedro R., Pedro G.: Seroprevalence of porcine epidemic diarrhea virus infection among different types of breeding swine farms in Spain. Prev Vet Med 1995, 23, 33–40.
  3. 3. Chen J., Wang C., Shi H., Qiu H., Liu S., Chen X., Zhang Z., Feng L.: Molecular epidemiology of porcine epidemic diarrhea virus in China. Arch Virol 2010, 155, 1471–1476.
  4. 4. Chen Q., Li G., Stasko J., Thomas J.T., Stensland W.R., Pillatzki A.E., Gauger P.C., Schwartz K.J., Madson D., Yoon K.J.: Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak in US swine. J Clin Microbiol 2013, 52, 234–243.
  5. 5. Chen X., Yang J., Yu F., Ge J., Lin T., Song T.: Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian, China. Virus Genes 2012, 45, 499–507.
  6. 6. Fan J.H., Zuo Y.Z., Li J.H., Pei L.H.: Heterogeneity in membrane protein genes of porcine epidemic diarrhea viruses isolated in China. Virus Genes 2012, 45, 113–117.
  7. 7. Gardner S.N., Kuczmarski T.A., Vitalis E.A., Slezak T.R.: Limitations of Taq-Man PCR for detecting divergent viral pathogens illustrated by hepatitis A, B, C, and E viruses and human immunodeficiency virus. J Clin Microbiol 2003, 41, 2417–2427.
  8. 8. Gu J.P., Yue X.W., Li C.Y., Xing R., Yuan C.L., Cui L., Hua X.G., Yang Z.B. Development and primary application of the SYBR Green I RT-qPCR assay for the detection of the transmissible gastroenteritis virus (TGEV) S gene. 4International Conference on Biomedical Engineering and Informatics (BMEI'11), 2011, Shanghai, pp. 1412–1415.
  9. 9. Huang Y.W., Dickerman A.W., Pineyro P., Li L., Fang L., Kiehne R., Opriessnig T., Meng X.J.: Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. mBio 2013, 4, e00737–13.
  10. 10. Kim S.H., Kim I.J., Pyo H.M., Tark D.S., Song J.Y., Hyn B.H.: Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus. J Virol Methods 2007, 146, 172–177.
  11. 11. Li W., Li H., Liu Y., Pan Y., Deng F., Song Y., Tang X., He Q.: New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis 2012, 18, 1350–1353.
  12. 12. Li Z., Chen F., Yuan Y., Zeng X., Wei Z., Zhu L., Sun B., Xie Q., Cao Y., Xue C., Ma J., Bee Y.: Sequence and phylogenetic analysis of nucleocapsid genes of porcine epidemic diarrhea virus (PEDV) strains in China. Arch Virol 2013, 158, 1267–1273.
  13. 13. Li Z.L., Zhu L., Ma J.Y., Zhou Q.F., Song Y.H., Sun B.L., Chen R.A., Xie Q.M., Bee Y.Z.: Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in south China. Virus genes 2012, 45, 181–185.
  14. 14. Oldham J.: Pig Farming (Oct suppl), 1972, 72–73.
  15. 15. Ouyang S.Y., Yang D., Ouyang H.S., Ma H.W.: Real-time fluorescent quantitative PCR technique and its application. Chem Life Sci 2004, 26, 74–76.
  16. 16. Pan X.T., Li W., Zhou Q.F., Wang D.D., Bi Y.Z., Chen F., Song Y.H.: Isolation and characterization of a variant porcine epidemic diarrhea virus in China. Virol J 2012, 9, 195–203.
  17. 17. Park S., Kim S., Song D., Park B.: Novel porcine epidemic diarrhea virus variant with large genomic deletion, South Korea. Emerg Infect Dis 2014, 20, 2089–2092.
  18. 18. Park S.J., Song D.S., Park B.K.: Molecular epidemiology and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea. Arch Virol 2013, 158, 1533–1541.
  19. 19. Reed L.J., Muench H.: A simple method of estimating fifty per cent endpoints. Am J Epidemiol 1938, 27, 493–497.
  20. 20. Ren X., Li P.: Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus. Virus Genes 2011, 42, 229–235.
  21. 21. Salin C., Thaweesak C., Mai L Q., Sudarat D., Arunee C., Alongkorn A., Olfert L., Thaweesak S., Apiradee T.B., Yong P.R. H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes. J Virol Methods 2007, 139, 44–49.
  22. 22. Song D., Park B.: Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus genes 2012, 44, 167–175.
  23. 23. Sun R.Q, Cai R.J., Chen Y.Q., Liang P.S., Chen D.K., Song C.X.: Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg Infect Dis 2012, 18, 161–163.
  24. 24. Zhang K., He Q.G.: Development and application of multiple RT-PCR assay for PEDV, TGEV and PoRV. Chin. J Anim Vet Sci 2010, 41, 1001–1005.
  25. 25. Zhang S.F., Jia Y., Wang M.X., Ni Y.X., He K.W., Chen F.Y.: Establishment of nested RT-PCR diagnostic method for porcine epidemic diarrhea virus. Virol Sinica 2004, 19, 174–176.
  26. 26. Zhao P.D., Bai J., Tang T.S., Li Y.F., Tan C. Shi X.: Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus. J Virol Methods 2014, 206, 150–155.
  27. 27. Zhou Y.J., Wu Y.L., Zhu J.P., Tong W., Yu H., Jiang Y.F., Tong G.Z.: Complete genome sequence of a virulent porcine epidemic diarrhea virus strain. J Virol 2012, 86, 13862.
DOI: https://doi.org/10.1515/jvetres-2016-0018 | Journal eISSN: 2450-8608 (formerly 2300-3235)
Language: English
Page range: 127 - 133
Submitted on: Sep 9, 2015
Accepted on: Apr 22, 2016
Published on: May 28, 2016
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services

© 2016 Yi-Xuan Hou, Chun Xie, Kang Wang, Yu-Ting Zhao, Yang-Yang Xie, Hong-Yan Shi, Jian-Fei Chen, Li Feng, Guang-Zhi Tong, Xiu-Guo Hua, Cong-Li Yuan, Yan-Jun Zhou, Zhi-Biao Yang, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.