Application of FTA® Cards for detection and storage of avian influenza virus

Jóźwiak, Michał; Wyrostek, Krzysztof; Domańska-Blicharz, Katarzyna; Olszewska-Tomczyk, Monika; Śmietanka, Krzysztof; Minta, Zenon

doi:10.1515/jvetres-2016-0001

Abstract

Introduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenicity H5N1 subtypes. Detection of viral material was conducted using molecular RT-PCR and rRT- PCR method. Results: The infectivity of LPAIV/H7N1 and HPAIV/H5N1 was completely inactivated within 1 h and 24 h after adsorption to FTA® Cards at room temperature, respectively. Viruses stored on FTA® Cards had detection limit approximately 1 log₁₀ lower than live viruses. Viral RNA of both strains were detectable on the cards by rRT-PCR for a minimum of 150 d, irrespectively of storage temperatures (room temperature, -20ºC). RNA was also detected in all samples obtained from SPF chickens experimentally infected with HPAI/H5N1 on 3^rd and 4^th day post-infection (p.i.).

Conclusion: FTA® Cards enable safe and effective alternative transport of samples for molecular diagnosis of AIV.

References

1. Abdelwhab E.M., Luschow D., Harder T.C., Hafez H.M.: The use of FTA® filter papers for diagnosis of avian influenza virus. J Virol Methods 2011, 174, 120-122.10.1016/j.jviromet.2011.03.01721419801
Search in Google Scholar
2. Act of 11 March 2004 on animal health protection and fighting against infectious animal diseases (Journal of Laws 2004, No. 69, item 625 with subsequent amendments).
Search in Google Scholar
3. Cortes A.L., Montiel E.R., Gimeno I.M.: Validation of Marek’s disease diagnosis and monitoring of Marek’s disease vaccines from samples collected in FTA® Cards. Avian Dis 2009, 54, 510-516.10.1637/8871-041009-Reg.120095150
Search in Google Scholar
4. Kalthoff D., Globie A., Beer M.: (Highly pathogenic) avian influenza as a zoonotic agent. Vet Microbiol 2010, 140, (3-4), 237-245.10.1016/j.vetmic.2009.08.02219782482
Search in Google Scholar
5. Keeler S., Ferro P., Brown J., Jackwood M., Stallknecht D.: Use of FTA® Sampling Cards for molecular detection of avian influenza virus in wild birds. Avian Dis 2012, 56, 200-207.10.1637/9862-072611-Reg.122545547
Search in Google Scholar
6. Kraus R.H., van Hooft P., Waldenström J., Latorre-Margalef N., Ydenberg R.C., Prins H.H.: Avian influenza surveillance with FTA Cards. Field methods, biosafety, and transportation issues solved. J Visual Exp 2011, 54, 1-5.10.3791/2832321112321847074
Search in Google Scholar
7. Moscoso H., Alvarado I., Hofacre C.L.: Molecular analysis of infectious bursal disease virus from bursal tissues collected on FTA® filter paper. Avian Dis 2006, 50, 391-396.10.1637/7505-011306R.117039839
Search in Google Scholar
8. Moscoso H., Raybon E.O., Thayer S.G., Hofacre C.L.: Molecular detection and serotyping of infectious bronchitis virus from FTA® filter paper. Avian Dis 2005 49, 24-29.10.1637/722015839408
Search in Google Scholar
9. Narayanan M.S., Parthiban M., Sathiya P., Kumanan K.: Molecular detection of Newcastle disease virus using Flinder Technology Associated-PCR. Vet Arch 2010, 80, 51-60.
Search in Google Scholar
10. Natarajan P., Trinh T., Mertz L., Goldsborough M., Fox D.K.: Paper-based archiving of mammalian and plant samples for RNA analysis. BioTechniques 2000, 29, 1328-1333.10.2144/00296pf0111126136
Search in Google Scholar
11. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). World Organization for Animal Health, Paris, 2015, Chapter 2.3.4, 1- 23.
Search in Google Scholar
12. Peiris M.J.S., De Jong D.M., Guan Yi.: Avian influenza virus (H5N1) a threat to human health. Clin Microbiol Rev 2007, 20, (2), 243-267.10.1128/CMR.00037-06186559717428885
Search in Google Scholar
13. Perozo F., Villegas P., Estevez C., Alvarado I., Purvis L.B.: Use of FTA filter paper for the molecular detection of Newcastle disease virus. Avian Pathol 2006, 35, 93-98.10.1080/0307945060059741016595299
Search in Google Scholar
14. Scientific Report of EFSA. Higly pathogenic avian influenza A subtype H5N8. EFSA J 2014, 12, 39-41.10.2903/j.efsa.2014.3941
Search in Google Scholar
15. Spackman E., Senne D.A., Myers T.J., Bulaga L.L., Garber L.P., Perdue M.L.: Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol 2002, 40, 3, 256-3260.10.1128/JCM.40.9.3256-3260.200213072212202562
Search in Google Scholar
16. Starick E., Römer-Oberdörfer A. Werner O.: Type- and subtype-specific RT-PCR assays for avian influenza A viruses (AIV). J Vet Med 2000, 47, 4, 295-301.10.1046/j.1439-0450.2000.00386.x10861198
Search in Google Scholar

Application of FTA® Cards for detection and storage of avian influenza virus

Abstract

Paradigm

My account