Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Dumache, Raluca; Rogobete, Alexandru Florin; Sandesc, Dorel; Bedreag, Ovidiu Horea; Ciocan, Veronica; Muresan, Camelia; Stan, Adrian Tudor; Sandesc, Mihai; Dinu, Anca; Popovici, Sonia Elena; Enache, Alexandra

doi:10.1515/jim-2017-0074

Abstract

The current practice in the field of forensic medicine imposes the use of modern investigation techniques. The complexity of laboratory investigation methods needed for a final result of the investigation in forensic medicine needed new biomarkers of higher specificity and selectivity. Such biomarkers are the microRNAs (miRNAs), short, non-coding RNAs composed of 19–24 nucleotides. Their characteristics, such as high stability, selectivity, and specificity for biological fluids, differ from tissue to tissue and for certain pathologies, turning them into the ideal candidate for laboratory techniques used in forensic medicine. In this paper, we wish to highlight the biochemical properties and the usefulness of miRNAs in forensic medicine.

References

1. Hanson EK, Lubenow H, Ballantyne J. Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Anal Biochem. 2009;387:303-314.10.1016/j.ab.2009.01.03719454234
Search in Google Scholar Back to article
2. Verduijn R. Recent Developments in Forensic Biology. BSc Biol. 2010.
Search in Google Scholar Back to article
3. Petersen CH, Hjort BB, Tvedebrink T, Kielpinski LJ, Vinther J, Morling N. Body fluid identification of blood, saliva and semen using second generation sequencing of micro-RNA. Forensic Sci Int Genet Suppl Ser. 2013;4:e204-e205.10.1016/j.fsigss.2013.10.105
Open DOI Search in Google Scholar Back to article
4. Wang Z, Zhang J, Luo H, Ye Y, Yan J, Hou Y. Screening and confirmation of microRNA markers for forensic body fluid identification. Forensic Sci Int Genet. 2013;7:116-123.10.1016/j.fsigen.2012.07.00622909992
Open DOI Search in Google Scholar Back to article
5. Meng L, Chen L, Li Z, Wu Z-X, Shan G. Roles of microRNAs in the Caenorhabditis elegans nervous system. J Genet Genomics. 2013;40:445-452.10.1016/j.jgg.2013.07.00224053946
Open DOI Search in Google Scholar Back to article
6. Zubakov D, Boersma AWM, Choi Y, Van Kuijk PF, Wiemer EC, Kayser M. MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation. Int J Legal Med. 2010;124:217-226.10.1007/s00414-009-0402-3285501520145944
Search in Google Scholar Back to article
7. Wang Z, Luo H, Pan X, Liao M, Hou Y. A model for data analysis of microRNA expression in forensic body fluid identification. Forensic Sci Int Genet. 2012;6:419-423.10.1016/j.fsigen.2011.08.00821903498
Open DOI Search in Google Scholar Back to article
8. Meng S, Cao JT, Zhang B, Zhou Q, Shen CX, Wang CQ. Downregulation of microRNA-126 in endothelial progenitor cells from diabetes patients, impairs their functional properties, via target gene Spred-1. J Mol Cell Cardiol. 2012;53:64-72.10.1016/j.yjmcc.2012.04.00322525256
Search in Google Scholar Back to article
9. Guo HQ, Huang GL, Guo CC, Pu XX, Lin TY. Diagnostic and prognostic value of circulating miR-221 for extranodal natural killer/T-cell lymphoma. Dis Markers. 2010;29:251-258.10.1155/2010/474692
Open DOI Search in Google Scholar Back to article
10. Deng H, Guo Y, Song H, et al. MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer. Gene. 2013;518:351-359.10.1016/j.gene.2012.12.10323333942
Search in Google Scholar Back to article
11. Uhlich RM, Konie JA, Davis JW, et al. Novel microRNA correlations in the severely injured. Surgery. 2014;156:834-840.10.1016/j.surg.2014.06.01725239329
Search in Google Scholar Back to article
12. Liu T, Cheng W, Gao Y, Wang H, Liu Z. Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Mol Med Rep. 2012;6:535-542.10.3892/mmr.2012.96722735917
Open DOI Search in Google Scholar Back to article
13. Chen W, Harbeck MC, Zhang W, Jacobson JR. MicroRNA regulation of integrins. Transl Res. 2013;162:133-143.10.1016/j.trsl.2013.06.008382555423859989
Search in Google Scholar Back to article
14. Abdelmohsen K, Srikantan S, Kang MJ, Gorospe M. Regulation of senescence by microRNA biogenesis factors. Ageing Res Rev. 2012;11:491-500.10.1016/j.arr.2012.01.003334899522306790
Open DOI Search in Google Scholar Back to article
15. Westholm JO, Lai EC. Mirtrons: microRNA biogenesis via splicing. Biochimie. 2011;93:1897-1904.10.1016/j.biochi.2011.06.017318518921712066
Open DOI Search in Google Scholar Back to article
16. Von Brandenstein M, Richter C, Fries JWU. MicroRNAs: Small but amazing, and their association with endothelin. Life Sci. 2012;91:475-489.10.1016/j.lfs.2012.06.02522771694
Open DOI Search in Google Scholar Back to article
17. Yi R, Qin Y, Macara IG, Cullen BR. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev. 2003;17:3011-3016.10.1101/gad.115880330525214681208
Search in Google Scholar Back to article
18. Cheng G. Circulating miRNAs: roles in cancer diagnosis, prognosis and therapy. Adv Drug Deliv Rev. 2015;81:75-93.10.1016/j.addr.2014.09.00125220354
Search in Google Scholar Back to article
19. Andersen HH, Duroux M, Gazerani P. MicroRNAs as modulators and biomarkers of inflammatory and neuropathic pain conditions. Neurobiol Dis. 2014;71:159-168.10.1016/j.nbd.2014.08.00325119878
Search in Google Scholar Back to article
20. Weilner S, Schraml E, Redl H, Grillari-Voglauer R, Grillari J. Secretion of microvesicular miRNAs in cellular and organismal aging. Exp Gerontol. 2013;48:626-633.10.1016/j.exger.2012.11.017369556623283304
Open DOI Search in Google Scholar Back to article
21. Rana S, Malinowska K, Zöller M. Exosomal tumor microRNA modulates premetastatic organ cells. Neoplasia. 2013;15:281-295.10.1593/neo.122010359315123479506
Open DOI Search in Google Scholar Back to article
22. Dumache R, Rogobete AF, Bedreag OH, et al. Use of miRNAs as biomarkers in sepsis. Anal Cell Pathol (Amst). 2015;2015:186716.10.1155/2015/186716449937526221578
Search in Google Scholar Back to article
23. Weber J, Baxter DH, Zhang S, et al. The MicroRNA Spectrum in 12 Body Fluids. Clin Chem. 2010;56:1733-1741.10.1373/clinchem.2010.147405484627620847327
Open DOI Search in Google Scholar Back to article
24. Hanson E, Lubenow H, Ballantyne J. Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Forensic Sci Int Genet Suppl Ser. 2009;2:503-504.10.1016/j.fsigss.2009.08.184
Open DOI Search in Google Scholar Back to article
25. Williams G, Uchimoto ML, Coult N, World D, Beasley E, Avenell P. Characterisation of body fluid specific microRNA markers by capillary electrophoresis. Forensic Sci Int Genet Suppl Ser. 2013;4:e274-e275.10.1016/j.fsigss.2013.10.140
Open DOI Search in Google Scholar Back to article
26. Kuner R, Brase JC, Sültmann H, Wuttig D: microRNA biomarkers in body fluids of prostate cancer patients. Methods. 2013;59:132-137.10.1016/j.ymeth.2012.05.00422652624
Search in Google Scholar Back to article
27. Gilad S, Meiri E, Yogev Y, et al. Serum microRNAs are promising novel biomarkers. PLoS One. 2008;3:1-7.10.1371/journal.pone.0003148251978918773077
Search in Google Scholar Back to article
28. Roth C, Rack B, Müller V, Janni W, Pantel K, Schwarzenbach H. Circulating microRNAs as blood-based markers for patients with primary and metastatic breast cancer. Breast Cancer Res. 2010;12:R90.10.1186/bcr2766304642921047409
Search in Google Scholar Back to article
29. Guo Z, Maki M, Ding R, Yang Y, Zhang B, Xiong L. Genome-wide survey of tissue-specific microRNA and transcription factor regulatory networks in 12 tissues. Sci Rep. 2014;4.10.1038/srep05150538149024889152
Search in Google Scholar Back to article
30. Sood P, Krek A, Zavolan M, Macino G, Rajewsky N. Cell-type-specific signatures of microRNAs on target mRNA expression. Proc Natl Acad Sci USA. 2006;103:2746-2751.10.1073/pnas.0511045103141382016477010
Search in Google Scholar Back to article
31. Bloomston M, WL F, Petrocca F, et al. MicroRNA expression patterns to differentiate pancreatic adenocarcinoma from normal pancreas and chronic pancreatitis. JAMA. 2007;297:1901-1908.10.1001/jama.297.17.190117473300
Search in Google Scholar Back to article
32. Tsai HL, Yang IP, Huang CW, et al. Clinical significance of microRNA-148a in patients with early relapse of stage II stage and III colorectal cancer after curative resection. Transl Res. 2013;162:258-268.10.1016/j.trsl.2013.07.00923933284
Search in Google Scholar Back to article
33. Harraz MM, Dawson TM, Dawson VL. MicroRNAs in Parkinson’s disease. J Chem Neuroanat. 2011;42:127-130.10.1016/j.jchemneu.2011.01.005316381321295133
Open DOI Search in Google Scholar Back to article
34. Papurica M, Rogobete AF, Sandesc D, et al. Advances in Biomarkers in Critical Ill Polytrauma Patients. Clin Lab. 2016;62:977-986.10.7754/Clin.Lab.2015.15110327468558
Search in Google Scholar Back to article
35. Jones K, Nourse JP, Keane C, Bhatnagar A, Gandhi MK. Plasma microRNA are disease response biomarkers in classical hodgkin lymphoma. Clinical Cancer Research. 2014;20:253-264.10.1158/1078-0432.CCR-13-102424222179
Open DOI Search in Google Scholar Back to article
36. Yang X, Salminen WF, Shi Q, et al. Potential of extracellular microRNAs as biomarkers of acetaminophen toxicity in children. Toxicol Appl Pharmacol. 2015;284:180-187.10.1016/j.taap.2015.02.013455862225708609
Search in Google Scholar Back to article
37. Wang F, Li C, Liu W, Jin Y. Effect of exposure to volatile organic compounds (VOCs) on airway inflammatory response in mice. J Toxicol Sci. 2012;37:739-748.10.2131/jts.37.73922863854
Search in Google Scholar Back to article
38. Cao Y, Yu SL, Wang Y, Guo GY, Ding Q, An RH. MicroRNA-dependent regulation of PTEN after arsenic trioxide treatment in bladder cancer cell line T24. Tumor Biol. 2011;32:179-188.10.1007/s13277-010-0111-z20857258
Open DOI Search in Google Scholar Back to article
39. Bollati V, Marinelli B, Apostoli P, et al. Exposure to metal-rich particulate matter modifies the expression of candidate MicroRNAs in peripheral blood leukocytes. Environ Health Perspect. 2010;118:763-768.10.1289/ehp.0901300289885120061215
Search in Google Scholar Back to article
40. Avissar-Whiting M, Veiga KR, et al. Bisphenol A exposure leads to specific microRNA alterations in placental cells. Reprod Toxicol. 2010;29:401-406.10.1016/j.reprotox.2010.04.004289687520417706
Open DOI Search in Google Scholar Back to article
41. Singh NP, Singh UP, Guan H, Nagarkatti P, Nagarkatti M. Prenatal Exposure to TCDD Triggers Significant Modulation of microRNA Expression Profile in the Thymus That Affects Consequent Gene Expression. PLoS One. 2012;7:e45054.10.1371/journal.pone.0045054344320823024791
Search in Google Scholar Back to article
42. Liu R, Zhang C, Hu Z, et al. A five-microRNA signature identified from genome-wide serum microRNA expression profiling serves as a fingerprint for gastric cancer diagnosis. Eur J Cancer. 2011;47:784-791.10.1016/j.ejca.2010.10.02521112772
Open DOI Search in Google Scholar Back to article
43. Zhang X, Cui L, Ye G, et al. Gastric juice microRNA-421 is a new biomarker for screening gastric cancer. Tumor Biol. 2012;33:2349-2355.10.1007/s13277-012-0497-x22926798
Search in Google Scholar Back to article
44. Song MY, Pan KF, Su HJ, et al. Identification of serum microRNAs as novel non-invasive biomarkers for early detection of gastric cancer. PLoS One. 2012;7:1-9.10.1371/journal.pone.0033608330385622432036
Search in Google Scholar Back to article
45. Huang Z, Huang D, Ni S, Peng Z, Sheng W, Du X. Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer. Int J Cancer. 2010;127:118-126.10.1002/ijc.2500719876917
Open DOI Search in Google Scholar Back to article
46. Zhu C, Tsao M. Prognostic markers in lung cancer: is it ready for prime time? Transl Lung Cancer Res. 2014;3:149-158.
Search in Google Scholar Back to article
47. Wang P, Yang D, Zhang H, et al. Early Detection of Lung Cancer in Serum by a Panel of MicroRNA Biomarkers. Clin Lung Cancer. 2014;16:313-319. e110.1016/j.cllc.2014.12.00625639977
Open DOI Search in Google Scholar Back to article
48. Rehbein G, Schmidt B, Fleischhacker M. Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer. Clin Chim Acta. 2015;450:78-82.10.1016/j.cca.2015.07.02726232753
Search in Google Scholar Back to article
49. Powrózek T, Krawczyk P, Kowalski DM, Winiarczyk K, Olszyna-Serementa M, Milanowski J. Plasma circulating microRNA-944 and microRNA-3662 as potential histologic type-specific early lung cancer biomarkers. Transl Res. 2015;166:315-323.10.1016/j.trsl.2015.05.00926079400
Search in Google Scholar Back to article
50. Zhou J, Yu L, Gao X, et al. Plasma MicroRNA Panel to Diagnose Hepatitis B Virus–Related Hepatocellular Carcinoma. J Clin Oncol. 2011;29:4781-4788.10.1200/JCO.2011.38.269722105822
Open DOI Search in Google Scholar Back to article
51. Vincent L, Vang D, Nguyen J, et al. Mast cell activation contributes to sickle cell pathobiology and pain in mice. Blood. 2013;122:1853-1862.10.1182/blood-2013-04-498105377249523775718
Search in Google Scholar Back to article
52. Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA. 2008;105:10513-10518.10.1073/pnas.0804549105249247218663219
Search in Google Scholar Back to article
53. Redova M, Sana J, Slaby O. Circulating miRNAs as new blood-based biomarkers for solid cancers. Futur Oncol. 2013;9:387-402.10.2217/fon.12.19223469974
Search in Google Scholar Back to article
54. Lavie L, Lavie P. Molecular mechanisms of cardiovascular disease in OSAHS: The oxidative stress link. Eur Respir J. 2009;33:1467-1484.10.1183/09031936.0008660819483049
Open DOI Search in Google Scholar Back to article
55. Fichtlscherer S, De Rosa S, Fox H, et al. Circulating microRNAs in patients with coronary artery disease. Circ Res. 2010;107:677-684.10.1161/CIRCRESAHA.109.21556620595655
Search in Google Scholar Back to article
56. Weber M, Baker MB, Patel RS, Quyyumi AA, Bao G, Searles CD. MicroRNA Expression Profile in CAD Patients and the Impact of ACEI/ARB. Card Res Pract. 2011;2011:532915.10.4061/2011/532915313798721785714
Search in Google Scholar Back to article
57. Cheng Y, Tan N, Yang J, et al. A translational study of circulating cell-free microRNA-1 in acute myocardial infarction. Clin Sci (Lond). 2010;119:87-95.10.1042/CS20090645359381520218970
Search in Google Scholar Back to article
58. Ai J, Zhang R, Li Y, et al. Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction. Biochem Biophys Res Commun. 2010;391:73-77.10.1016/j.bbrc.2009.11.00519896465
Search in Google Scholar Back to article
59. Olivieri F, Antonicelli R, Lorenzi M, et al. Diagnostic potential of circulating miR-499-5p in elderly patients with acute non ST-elevation myocardial infarction. Int J Cardiol. 2013;167:531-536.10.1016/j.ijcard.2012.01.07522330002
Search in Google Scholar Back to article
60. Cogswell JP, Ward J, Taylor IA, et al. Identification of miRNA changes in Alzheimer’s disease brain and CSF yields putative biomarkers and insights into disease pathways. J Alzheimers Dis. 2008;14:27-41.10.3233/JAD-2008-14103
Open DOI Search in Google Scholar Back to article
61. Karolina DS, Armugam A, Tavintharan S, et al. MicroRNA 144 impairs insulin signaling by inhibiting the expression of insulin receptor substrate 1 in type 2 diabetes mellitus. PLoS One. 2011;6.10.1371/annotation/698b7123-174f-4a09-95c9-fd6f5017d622
Search in Google Scholar Back to article
62. Bedreag OH, Sandesc D, Chiriac SD, et al. The Use of Circulating miRNAs as Biomarkers for Oxidative Stress in Critically Ill Polytrauma Patients. Clin Lab. 2016;62:263-274.10.7754/Clin.Lab.2015.150740
Search in Google Scholar Back to article
63. Sun T, Chen X, Liu Z, et al. Expression profiling of MicroRNAs in hippocampus of rats following traumatic brain injury. J Huazhong Univ Sci Technol Medical Sci. 2014;34:548-553.10.1007/s11596-014-1313-125135725
Open DOI Search in Google Scholar Back to article
64. Butcher N, Balogh ZJ. The definition of polytrauma: the need for international consensus. Injury. 2009;40:S12-S22.10.1016/j.injury.2009.10.03219895948
Search in Google Scholar Back to article
65. Liu NK, Wang XF, Lu B, Xu XM. Altered microRNA expression following traumatic spinal cord injury. Exp Neurol. 2009;219:424-429.10.1016/j.expneurol.2009.06.015281050819576215
Search in Google Scholar Back to article
66. Huang C, Xiao X, Chintagari NR, Breshears M, Wang Y, Liu L. MicroRNA and mRNA expression profiling in rat acute respiratory distress syndrome. BMC Med Genomics. 2014;7:1-15.10.1186/1755-8794-7-46412853625070658
Open DOI Search in Google Scholar Back to article
67. Ndegwa LK, Katz MA, McCormick K, et al. Surveillance for respiratory health care-associated infections among inpatients in 3 Kenyan hospitals. Am J Infect Control. 2014;42:985-990.10.1016/j.ajic.2014.05.02225179331
Search in Google Scholar Back to article
68. Wang J, Yu M, Yu G, et al. Serum miR-146a and miR-223 as potential new biomarkers for sepsis. Biochem Biophys Res Commun. 2010;394:184-188.10.1016/j.bbrc.2010.02.14520188071
Search in Google Scholar Back to article
69. Vasilescu C, Rossi S, Shimizu M, et al. MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis. PLoS One. 2009;4:e7404.10.1371/journal.pone.0007405275662719823581
Search in Google Scholar Back to article
70. Schmidt WM, Spiel AO, Jilma B, Wolzt M, Müller M. In vivo profile of the human leukocyte microRNA response to endotoxemia. Biochem Biophys Res Commun. 2009;380:437-441.10.1016/j.bbrc.2008.12.19019284987
Search in Google Scholar Back to article

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Abstract

Paradigm

My account