The Effect of the Antioxidant Drug “U-74389G” on Haemoglobin Levels Following a Hypoxemia/ Re-oxygenation Protocol in Rats

Tsompos, Constantinos; Panoulis, Constantinos; Toutouzas, Konstantinos; Zografos, George; Papalois, Apostolos

doi:10.1515/jccm-2015-0017

Abstract

Critically ill patients usually present with circulatory hypoxemia and this is associated with a poorer prognosis. The aim of this experimental study was to examine the effect of U-74389G with specific regard to a hypoxemia/re-oxygenation protocol, on mean blood haemoglobin (Hgb) levels in rats.

Materials and methods: Forty rats (mean weight 231.9 g) were used in the study. Hgb levels were measured at sixty minutes (groups A and C) and at 120 minutes (groups B and D) of re-oxygenation. U-74389G was administered only in groups C and D.

Results: U-74389G administration non-significantly increased the Hgb levels by 3.95+2.10% (p=0.0604). Re-oxygenation time non-significantly increased the Hgb levels by 3.39+2.12% (p=0.1285). U-74389G administration and reoxygenation time together, significantly increased the Hgb levels by 2.55%+1.25% (p=0.0423).

Conclusions: Results of this study indicate that U-74389G administration, re-oxygenation time, but mainly their interaction significantly increase the Hgb levels within the studied time limits.

References

1. Shi F, Cavitt J, Audus KL. 21-aminosteroid and 2-(aminomethyl) chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers. Free Radic Biol Med. 1995;19:349-57.10.1016/0891-5849(95)00049-4
Search in Google Scholar
2. Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A. The Hypoglucemic Effect of the Antioxidant Drug “U-74389g” during Ischemia Reperfusion Injury in Rats. Int J Diabetes Clin Res. 2015;2:1-4.10.23937/2377-3634/1410031
Search in Google Scholar
3. Barshishat-Kupper M, Mungunsukh O, Tipton AJ, et al. Captopril modulates hypoxia inducible factors and erythropoietin responses in a murine model of total body irradiation. Exp Hematol. 2011;39:293-304.10.1016/j.exphem.2010.12.002
Search in Google Scholar
4. Weltert L, D’Alessandro S, Nardella S, et al. Preoperative very short-term, high-dose erythropoietin administration diminishes blood transfusion rate in off-pump coronary artery bypass: a randomized blind controlled study. J Thorac Cardiovasc Surg. 2010;139:621-7.10.1016/j.jtcvs.2009.10.012
Search in Google Scholar
5. Gardin C, Fenaux P. Supportive care including growth factors in myelodysplastic syndromes. Rev Clin Exp Hematol. 2004;8:E3.10.1201/b14440-41
Search in Google Scholar
6. Ferber A, Minior VK, Bornstein E, et al. Fetal “nonreassuring status” is associated with elevation of nucleated red blood cell counts and interleukin-6. Am J Obstet Gynecol. 200;192:1427-9.10.1016/j.ajog.2004.12.076
Search in Google Scholar
7. Fujishiro N, Endo Y, Warashina A, et al. Mechanisms for hypoxia detection in O2-sensitive cells. Jpn J Physiol. 2004;54:109-23.10.2170/jjphysiol.54.109
Search in Google Scholar
8. Vatansever U, Acunaş B, Demir M, et al. Nucleated red blood cell counts and erythropoietin levels in high-risk neonates. Pediatr Int. 2002;44:590-5.10.1046/j.1442-200X.2002.01630.x
Search in Google Scholar
9. Singbartl G. Adverse events of erythropoietin in long-term and in acute/short-term treatment. Clin Investig. 1994;72(6 Suppl):S36-43.
Search in Google Scholar
10. Hara H, Ogawa M. Erythropoietic precursors in mice under erythropoietic stimulation and suppression. Exp Hematol. 1977;5:141-8.
Search in Google Scholar
11. Haddad J Jr, Egusa K, Takoudes TG. Effects of 21-aminosteroid U-74389G on acute otitis media in a guinea pig model. Otolaryngol Head Neck Surg. 1998;118:44-8.10.1016/S0194-5998(98)70373-0
Search in Google Scholar

The Effect of the Antioxidant Drug “U-74389G” on Haemoglobin Levels Following a Hypoxemia/ Re-oxygenation Protocol in Rats

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