Endothelium and the effect of activated neutrophils on arterial smooth muscle

Bauer, Viktor; Sotnikova, Ruzena

doi:10.1515/intox-2015-0008

Abstract

The aim of the study was to analyze the involvement of the endothelium in the effects of neutrophils (PMNL) on phenylephrine-pre-contracted isolated rings of the rat thoracic aorta and to compare their effects with those of peroxynitrite (ONOO⁻) and hypochlorous acid (HOCl). Activated PMNL-induced contraction of the precontracted aorta was prevented by the blockade of NO-synthase and by endothelium removal. In the endothelium-free preparations, the effect of PMNL reappeared in the presence of sodium nitroprusside. The effect of ONOO⁻ and HOCl significantly differed from that of activated PMNL both in the presence and absence of the endothelium. It is therefore likely that neither ONOO⁻ nor HOCl generated by transformation of superoxide anion radical (O₂^•−) produced by PMNL is involved in their action. Reduction of the relaxant effect of nitric oxide derived from the endothelium by O₂^•− seems to be the keystone mechanism in generation of PMNL-induced contraction.

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Endothelium and the effect of activated neutrophils on arterial smooth muscle

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