Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats

Koriem, Khaled M. M.; Gad, Islam B.; Nasiry, Zayenab K.

doi:10.1515/intox-2015-0006

Abstract

Cupressus sempervirens (C. sempervirens) belongs to the family Cupressaceae. It is widspread in Northern Africa, Greece, Turkey, North America, Cyprus and Syria. Cupressuflavone is the major ingredient of the plant leave extract. The aim of the present study was to evaluate the antiulcerogenic activity of the extract of C. sempervirens leaves in gastric ulcer tissues induced by indomethacin. The results of the present study revealed that indomethacin significantly decreased glutathione S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), reduced glutathione (GSH), glutathione reductase (GR) and superoxide dismutase (SOD) levels, while it increased significantly lipid peroxidation (MDA), nitric oxide (NO) and protein carbonyl (PC) levels in gastric tissue. Furthermore, indomethacin decreased p53 expression, while it increased bcl-2 expression in gastric tissue. Pretreatment with 5%, 10% & 20% of the LD₅₀ of C. sempervirens and cupressuflavone of indomethacin-treated rats restored all the above parameters to approach normal values. C. sempervirens at the highest dose was more effective than the two lower doses. C. sempervirens proved more potent than cupressuflavone. In conclusion, C. sempervirens exerted antiulcerogenic activity and the effect was dose-dependent and related to the cupressuflavone ingredient of the plant leave extract.

References

Aebi H. (1984). Catalase in vitro. Method Enzymol105: 121–126.
Search in Google Scholar
Agrawal PK. (1989). Carbon-13 NMR of Flavonoids, Elservier Science, New York, NY 10010, USA pp. 274–280.
Search in Google Scholar
Ames BN, Shigenaga MK, Hagen TM. (1993). Oxidants, antioxidants and the degenerative diseases of aging. Proc. Natl. Acad. Sci90: 7915–7922.
Search in Google Scholar
Chaudhary SC, Siddiqui MS, Athar M, Alam MS. (2012). D-Limonene modulates inflammation, oxidative stress and Ras-ERK pathway to inhibit murine skin tumorigenesis. Hum Exp Toxicol90: 17–21.
Search in Google Scholar
Chevallier A. (1996). The Encyclopedia of Medicinal Plants Dorling Kindersley. London pp. 115–123.
Search in Google Scholar
Chiej R. (1984). Encyclopaedia of medicinal plants. MacDonald. London pp. 54–62.
Search in Google Scholar
Chopra RN, Nayar SL, Chopra IC. (1986). Glossary of Indian Medicinal Plants (Including the Supplement). New Delhi, India: Council of Scientific and Industrial Research, pp. 402–405.
Search in Google Scholar
Das D, Bandyopadhyay D, Bhattacharjee M, Banerjee RK. (1997). Hydroxyl radical is the majorcausative factor in stress-induced gastric ulceration. Free radical Biol Med23: 8–18.
Search in Google Scholar
Duke JA. (2002). Handbook of Medicinal Herbs, 2^nd Edn, CRC Press, Boca Raton, Florida pp. 238–246.
Search in Google Scholar
Elias JM, Margiotta M, Gabore D. (1989). Sensitivity and detection efficiency of the peroxidase antiperoxidase (PAP) avidin-biotin peroxidase complex (ABC), and peroxidase-labelled avidin-biotin (LAB) methods. Am J Clin Pathol92(1): 62–67.
Search in Google Scholar
Ellman GL. (1959). Tissue sulphydryl groups. Arch Biochem Biophys82: 70–77.
Search in Google Scholar
Emami SA, Asili J, Mohagheghi Z, Hassanzadeh, MK. (2007). Antioxidant activity of leaves and fruits of iranian conifers. Avid Based Compl Altern Med4: 313–319.
Search in Google Scholar
Esterbauer H, Schaur RJ, Zollner H. (1991). Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med1(1): 81–128.
Search in Google Scholar
Farnsworth NR. (2001). ed. NAPRALERT Database. Chicago, IL: University of Illinois at Chicago, pp. 47–51.
Search in Google Scholar
Fridovich I. (1983). Superoxide radical as an endogenous toxicant. Ann Rev Pharmacol Toxicol23: 239–257.
Search in Google Scholar
Goldberg DM, Spooner RJ. (1983). In “ Methods of enzymatic analysis (Bergmeyen, H. V. Ed) 3^rd Ed. Verlog Chemie, Deerfield Beach, Fl. 3: 258–265.
Search in Google Scholar
Gornall AC, Bardawill CJ, David MM. (1949). Determination of serum proteins by means of the Biuret-reaction. J Biol Chem177: 751–766.
Search in Google Scholar
Gu Q, Hu C, Chen Q, Xia Y, Feng J and Yang H. (2009). Development of a rat model by 3, 4-benzopyrene intra-pulmonary injection and evaluation of the effect of green tea drinking on p53 and bcl-2 expression in lung carcinoma. Cancer Detect Prev32 (5–6): 444–451.
Search in Google Scholar
Harborne JB. (1993). The Flavonoids Advaces in Research, Chapman and Hall, London, pp. 460–467.
Search in Google Scholar
Hortus EZ. (1976). Liberty Hyde Bailey Hortorium, MacMillan Publishing Company, New York, USA ed. III., pp. 83–89.
Search in Google Scholar
Hsu SM, Raine L. (1984). In “Advances in Immunochem DeLellis, R. A. Ed Mason Publishing USA, Inc., New York, pp. 31–42.
Search in Google Scholar
Hussein AM, Ahmed OM. (2010). Regioselective one-pot synthesis and anti-proliferative and apoptosis effects of some novel tetrazolo[1, 5-a]pyrimidine derivatives. Bioorg Med18(7): 2639–2644.
Search in Google Scholar
Ibrahim NA, El-Seedi HR, Mohammed MM. (2007). Phyto-chemical investigation and hepato-protective activity of Cupressus sempervirens L. leaves growing in Egypt. Natl Prod Res21: 857–866.
Search in Google Scholar
Isenberg JI, McQuaid KR, Laine L, Walsh JH. (1995). Acid peptic disorders. In: Lippincott, J. B. (Eds) Yamada’s Text Book of Gastroenterology. Philadelphia, pp. 1347–1430.
Search in Google Scholar
Kassem FF, Harraz F M, El-Sebakhy N A, De Pooter H L, Schamp N M, Abu-Shleib H. (1991). Composition of the essential oil of Egyptian Cupressus sempervirens L. cones. Flavour Fragr J6 : 205–207.
Search in Google Scholar
Koriem KMM. (2009). Lead toxicity and the protective role of Cupressus sempervirens seeds growing in Egypt. Rev Latinoamer. Quím37(3): 230–242.
Search in Google Scholar
Koriem KM, Arbid MS, El-Gendy NF. (2010). The protective role of Tropaeolum majus on blood and liver toxicity induced by diethyl maleate in rats. Toxicol Mech Methods20: 579–586.
Search in Google Scholar
Leung AY, Foster S. (1996). Encyclopedia of common natural ingredients used in food, drugs and cosmetics. New York, NY: John Wiley and Sons, pp. 84–92.
Search in Google Scholar
Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. (1990). Determination of carbonyl content in oxidatively modified proteins. Method Enzymol186: 464–78.
Search in Google Scholar
Mannervik B, Guthenberg C. (1981). Glutathione transferase (human placenta). Methods Enzymol77: 231–235.
Search in Google Scholar
Miura T, Muraoka S, Fujimoto Y. (2002). Lipid peroxidation induced by indomethacin with horseradish peroxidase and hydrogen peroxide: involvement of indomethacin radicals. Biochem Pharmacol63(11): 2069–74.
Search in Google Scholar
Moon HK, Yang ES, Park JW. (2006). Protection of peroxynitrite-induced DNA damage by dietary antioxidants. Arch Pharmacol Res29: 213–7.
Search in Google Scholar
Navickiene HMD, Lopes LMX. (2001). Alkamides and phenethyl derivatives from Aristolochia gehrtii. J Braz Chem Soc12: 467–472.
Search in Google Scholar
Odabasoglu F, Cakir A, Suleyman H, Aslan A, Bayir Y, Halici M, Kazaz C. (2006). Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer in rats. J Ethnopharmacol103(1): 59–65.
Search in Google Scholar
Okhawa H, Ohishi N, Yagi K. (1979). Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem95: 351–358.
Search in Google Scholar
Omata M, Liew C. T, Asheavai M, Peters RL. (1980). Nonimmunologic binding of horseradish peroxidase to hepatitis B surface antigen: A possible source of error in immunohistochemistery. Am J Clin Pathol73(5): 626–632.
Search in Google Scholar
Pagalia DE, Valentine WN. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med70: 158–169.
Search in Google Scholar
Pennington SN, Smith CP. (1978). Indomethacin stimulation of lipid peroxidation and chemilumine-scense in rat liver microsomes. Lipids13(10): 636–643.
Search in Google Scholar
Rizk MA, Abdallah MS, Sharara HM, Ali. SA, Ibrahim NA, Mostafa MM. (2007). Efficiency of Cupressus sempiverenes L. and Juniperus Phoenicea against carbon tetrachloride hepatotoxicity in rats. Trends Med Res2(2): 83–94.
Search in Google Scholar
Said.OS, Khalil M, Fulder H. (2002). Ethnopharmacological survey of medicinal plants in Israel, the Golan heights and the west Bank region. J Ethanopharmacol83: 251–265.
Search in Google Scholar
Sivaramakrishnan V, Narasimha P, Shilpa M, Kumar VR, Devaraj SN. (2008). Attenuation of N-nitrosodiethylamine-induced hepatocellular carcinogenesis by a novel flavonol Morin. Chem Biol Inter171: 79–88.
Search in Google Scholar
Suttle NF. (1986). Copper deficiency in ruminants; recent developments. Veter Record119: 519–522.
Search in Google Scholar
Toma W, Gracioso J, Andrade FD, Hiruma-Lima CA, Vilegas W, Souza BA. (2002). Antiulcerogenic activity of four extracts obtained from the bark wood of Quassia amara L. (Simaroubaceae). Biol Pharm Bull25: 1151–1155.
Search in Google Scholar
Tweddle DA, Malcolm AJ, Cole M, Pearson AD, and Lunee J. (2001). p53 cellular localization and function in neuroblastoma. Am J Pathol158 (6): 2067–2077.
Search in Google Scholar
Uncini MRE, Camangi K, Tomei PE. (2001). Curing animals with plants: traditional usage in Tuscany (Italy). J Ethnopharmacol78: 71–76.
Search in Google Scholar
Uphof JCT. (1959). Dictionary of Economic Plants. Weinheim. New York. Hafner Publ. Co., pp. 73–79.
Search in Google Scholar
Usher GA. (1974). Dictionary of Plants Used by Man. London: Constable and Company, pp. 152–158.
Search in Google Scholar
Vidakovic M. (1991). Morphology and Variation. Annales forestales. Analiza šumar17: 2–11.
Search in Google Scholar
Waisel Y, Epstein V. (2000). How to reduce air pollution by Cupressus pollen? Allerg. Immunol (Paris)32(3): 141–142.
Search in Google Scholar
Wiegand H, Wagner AE, Boesch-Saadatmandi C. (2009). Effect of dietary genistein on Phase II and antioxidant enzymes in rat liver. Can Gen Prot6: 85–92.
Search in Google Scholar
Ye F, Chen y, Hoang T, Montgomery RL, Zhao XH, Bu H, Hu T, Taketo MM, van-Es JH, Clevers H, Hsieh J, Bassel-Duby R, Olson EN, Lu QR. (2009). HDAC1 and HDAC2 regulate oligodendrocyte differentiation by disrupting the β-catenin–TCF interaction. Nature Neurosci12: 829–838.
Search in Google Scholar
Yerlikaya A, Okur E, Ulukaya E. (2012). The p53-independent induction of apoptosis in breast cancer cells in response to proteosome inhibitor bortezomib. Tumor Biol37: 58–64.
Search in Google Scholar
Yin H, Pan X, Song Z, Wang S, Yang L, Sun G. (2014). Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells. Nutrients6(2): 564–574.
Search in Google Scholar

Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats

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