Fig. 1
The applied primers characteristics for multiplex PCR
| Parasite | Target | Name | Primer sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|---|
| E. multilocularis | nadl | Cesti | TGCTGATTTGTTAAAGTTAGTGATC | 395 bp |
| Cest2 | CATAAATCAATGGAAACAACAACAAG | |||
| E. granulosus | rrnS | Cest4 | GTTTTTGTGTGTTACATTAATAAGGGTG | 117 bp |
| Cest5 | GCGGTGTGTACMTGAGCTAAAC | |||
| Taenia spp. | rrnS | Cest3 | YGAYTCTTTTTAGGGGAAGGTGTG | 267 bp |
| Cest5 | GCGGTGTGTACMTGAGCTAAAC |
Prevalence of infectivity of domestic dogs in north of Iran using multiplex PCR from DNA of taeniid eggs from faecal samples according to sex and age (N=100)
| Infection | Sex | Age | Total | ||
|---|---|---|---|---|---|
| Male | Female | 12 month> (n: 21) | 12 month ≤(n: 79) | ||
| E. granulosus | 8.9 % (5) | 15.9 % (7) | 14.2 % (3) | 11.3 % (9) | 12 % (12) |
| Taenia spp. | 12.5 % (7) | 6.8 % (3) | 9.5 % (2) | 10.1 % (8) | 10 % (10) |
| E. multilocularis | 0 | 0 | 0 | 0 | 0 |
| Mixed infection | 3.5 % (2) | 0 | 4.7 % (1) | 1.2 % (1) | 2 % (2) |
| p value | >0.05 | >0.05 | |||
| Total | 24.9 % (14) | 22.7 % (10) | 27.4 % (6) | 22.6 % (18) | 24 % (24) |