Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Indirect haemagglutination assay (IHA) and enzyme-linked immunosorbent assay (ELISA) seroprevalence of Schistosomiasis in Najran region, Southern Kingdom of Saudi Arabia

			Titer
Taete	Number of total samples	Number of positive samples (%)	1:1280	1:640	1:320	1:160	1:1280
IHA	180	36 (20)	14	6	4	12	14
Eliza	180	42(23.3)	-	-	-	-	-
P-value= 0.44

Table 5

Diagnostic performance of indirect hemagglutination assay (IHA) and enzyme-linked immunosorbent assay IgG (ELISA IgG) of participants (n=180) with antibodies against schistosomiasis

Variables	ELISA IgG
Serological tests	-VE (Negative) n=138	+VE (Positive) n=42	Sensitivity (Se)	Specifi city (Sp)	_K value	SE of kappa
IHA(+VE)	0	36
IHA(-VE)	138	6	0.857(0.714 – 0.945)	0.1 (0.973 – 0.1)	0.902	0.039

Numbers in parentheses are 95 % confidence interval, note-sensitivity=true positives detected/(true positives + false negatives); specificity=true negatives detected/(true negatives + false positives), SE – standard error

Table 2

Seroprevalence and frequency of anti-Schistosoma mansoni IgG antibody (ELISA Test) among different age groups of participants from the Najran region, Southern Kingdom of Saudi Arabia

Age range (years)	No. tested	No. positive	%	No. negative	%	OR (95% confidence)	95% CI	P-value
10 – 19*	60	6a	10	54	90
20 – 40	60	24b, c	40	36	60	6.0	2.232 – 16.132	<0.001
>40	60	₁₂c	20	48	80	2.25	0.784 – 6.456

* Reference category, OR-odds ratio, CI – confidence interval

a value with different superscript in the same column differ at p<0.05

b value with different superscript in the same column differ at p<0.05

c value with different superscript in the same column differ at p<0.05

Table 3

Distribution ofanti-SchistosomamansoniIgG with socio-demographic variables, among examined participant from the Najran region, Southern Kingdom of Saudi Arabia

Variables	Schistosomiasis
Variables	No. examined	Infected n (%)	P- value
Sex
Male	100	36 (36)
Female	80	6(7.5)	<0.001
Site of residence
Aluraysah	66	16(24.2)
Al Khaledia	30	6(20)
Alfyselia	48	14(29.2)	0.516
El-ballad	36	6(16.7)
Fathers’ educational levels
Primary	60	24 (40)
Secondary	60	15(25)	<0.001
University	60	3(5)

Table 4

Comparison of Saudi and Non-Saudi residents with IgG seropositivity to Schistosoma mansoni

Nationality	No. examined	Infected n (%)	P- value
Saudi
Male	30	4 (13.3)a
Female	30	0 (0)b
Total	60	4 (7)
			<0.005
Non-Saudi
Male	70	32 (46)c
Female	50	6 (12)d
Total	120	38 (32)

a value with different superscript in the same column differ at p<0.05

b value with different superscript in the same column differ at p<0.05

c value with different superscript in the same column differ at p<0.05

d value with different superscript in the same column differ at p<0.05

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.1515/helmin-2016-0007 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

Journal RSS Feed

Language: English

Page range: 172 - 179

Submitted on: May 28, 2015

Accepted on: Jan 21, 2016

Published on: Apr 22, 2016

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Indirect hemagglutination assay (IHA),

Enzyme-linked immunosorbent assay (ELISA),

Related subjects:

Zoology,

Ecology,

Life sciences, other,

Medicine,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2016 I. S. El-Shahawy, M. I. Khalil, A. S. Adail, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 53 (2016): Issue 2 (June 2016)

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Figures & Tables

Fig. 1

Table 1

Table 5

Table 2

Table 3

Table 4

Paradigm

My account