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Detrimental effects of geldanamycin on adults and larvae of Trichinella spiralis

Helminthologia
Volume 53 (2016): Issue 2 (June 2016)
By:
Open Access
|Apr 2016

Figures & Tables

Fig. 1

Photomicrographs of skeletal muscle sections 5 weeks p.i. showing: A,C encapsulated larvae from the control infected mice. The collagenous capsule (thick arrow) surrounds sections of Trichinella larvae (thin arrow) (PAS, H&E × 400). B,D thinning of the capsule (thick arrow) around the larvae (thin arrow) in the treated mice (PAS, H&E × 400). E, mild to moderate inflammatory reactions around larvae in the control group (H&E × 100). F, moderate to marked intensity of inflammation around the encapsulated larvae and in between muscle fibres in the treated group (H&E × 65)
Photomicrographs of skeletal muscle sections 5 weeks p.i. showing: A,C encapsulated larvae from the control infected mice. The collagenous capsule (thick arrow) surrounds sections of Trichinella larvae (thin arrow) (PAS, H&E × 400). B,D thinning of the capsule (thick arrow) around the larvae (thin arrow) in the treated mice (PAS, H&E × 400). E, mild to moderate inflammatory reactions around larvae in the control group (H&E × 100). F, moderate to marked intensity of inflammation around the encapsulated larvae and in between muscle fibres in the treated group (H&E × 65)

Fig. 2

Electron micrographs ofthebody wall of adult worms (A-D) and larvae (E,F):A, section in the body wall of an adult from control infected mouse (× 12,000). B, loss of the electron dense layer in the cuticle (arrow) is observed (× 25,000). C, separation of the cuticle from the underlying hypodermis (arrow) is noted (× 6000). D, there are blunting of the epicuticle and washed-out image of the cytoplasm (arrow) (× 10,000). E, section in the cuticle of a larva from the control group (× 20,000). F, disruption of the layers of the cuticle (arrows) is noted (× 25,000)
Electron micrographs ofthebody wall of adult worms (A-D) and larvae (E,F):A, section in the body wall of an adult from control infected mouse (× 12,000). B, loss of the electron dense layer in the cuticle (arrow) is observed (× 25,000). C, separation of the cuticle from the underlying hypodermis (arrow) is noted (× 6000). D, there are blunting of the epicuticle and washed-out image of the cytoplasm (arrow) (× 10,000). E, section in the cuticle of a larva from the control group (× 20,000). F, disruption of the layers of the cuticle (arrows) is noted (× 25,000)

Muscle larval count in control and treated groups

  Control (n=7)Group 1 (n=7)Group 2 (n=7)
Mean ± SD25,428 ± 1,39715,428 ± 9758,142 ± 1,069
IE---------40.4 %67.9 %
F. test  14.526  
P. value  0.001  
Control & G1Control & G2G1 & G2
0.0010.0010.001
DOI: https://doi.org/10.1515/helmin-2016-0003 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 126 - 132
Submitted on: Dec 3, 2015
Accepted on: Feb 22, 2016
Published on: Apr 22, 2016
Published by: Slovak Academy of Sciences, Mathematical Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Geldanamycin,
Heat shock proteins,
Electron microscopy
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2016 A. A. Othman, Z. S. Shoheib, published by Slovak Academy of Sciences, Mathematical Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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