Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep Cover

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Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep

Volume 53 (2016): Issue 2 (June 2016)

By: M. Urda Dolinská, A. Königová, M. Babják and M. Várady

Open Access

|Apr 2016

Figures & Tables

Resistance factors for ivermectin (IVM) in the larval migration inhibition test

Strains	IVM RF₅₀	IVM RF₉₉
WR/McM	3.68	3.13
CAVR/McM	2.71	7.17
MOX23/McM	2.06	12.27
ISE-SL/McM	3.54	17.20
ISE-SLI/McM	4.90	8.16
WR/ISE	3.04	2.92
CAVR/ISE	2.24	6.70
MOX23/ISE	1.70	11.45
ISE-SL/ISE	2.92	16.06
ISE-SLI/ISE	4.04	7.62

Mean reduction (%) in motility of L3 larvae of resistant and susceptible strains of H_ contortusafter incubation in different concentrations of eprinomectin (EPM)

Concentration of EPM (μg/ml)
Isolate	0.1	1	10	100	200	300	LD₅₀
WR		43.4	43	53.3	72.2	71.7	41.04
CAVR	35		49.48	29.3	65.3	64.7	49.77
McM	0	37.1	76.4	63.9	100	100	3.98
ISE	34.6	53.23	65.2	69.3	100	100	1.54
MOX23	15.8	27.7	40.3	70.7	100	100	15.4
ISE SL	14.26	11.65	68.5	50.16	45.1	66.9	47.27

Resistance factors for ivermectin (IVM) and eprinomectin (EPM) in the micromotility test

Strains	IVM RF₅₀	EPM RF₅₀
WR/McM	57.81	10.31
CAVR/McM	2.67	12.50
MOX23/McM	1.00	3.87
ISE-SL/McM	49.89	11.88
WR/ISE	108.05	26.65
CAVR/ISE	5.00	32.32
MOX23/ISE	1.87	10.00
ISE-SL/ISE	93.25	30.69

Origin and status of anthelmintic susceptibility (S) or resistance (R) of ISE, McMaster, ISE-SL, ISE-SLI, MOX-23, WR and CAVR isolates of Haemonchus contortus

Isolate	R/S	Reference	Origin
ISE	susceptible	Roos et al. (2004)	Kenya
McMaster	susceptible	CSIRO, Armidale	Australia
ISE-SL	IVM	Coles (2005)	Great Britain
ISE-SLI	IVM	Coles (2005)	Great Britain
MOX-23	IVM	Prichard et al. (2002)	Canada
WR	BZ, CLO, IVM, RAF	Van Wyk and Malan (1988)	South Africa
CAVR	IVM, BZ	LeJambre et al. (1995)	Australia

Arithmetic mean ± SD of LD50 and LD99 for susceptible and resistant isolates obtained in the larval migration inhibition test with ivermectin

Isolate	LD₅₀(μg/ml) ± SD	LD₉₉(μg/ml) ± SD
McMaster	1.03 ±0.72	2.80 ±0.61
ISE	1.25 ±0.48	3.00 ±1.24
MOX23	2.13 ±1.77	34.36 ±25.14
CAVR	2.80 ± 0.72	20.10 ±12.12
WR	3.80 ± 0.35	8.77 ± 4.92
ISE-SL	3.65 ± 0.48	48.18 ±57.59
ISE-SLI	5.05 ±1.98	22.87 ± 7.45

Mean reduction (%) in motility of L3 larvae of resistant and susceptible strains of H_ contortusafter incubation in different concentrations of ivermectin (IVM)

Concentration of IVM (μg/ml)
Isolate	0.1	1	10	100	LD₅₀
WR	0	0	26.2	49.7	90.76
CAVR	0	55.2	52.8	76.1	4.2
McMaster	0	39.7	88.9	80.9	1.57
ISE	0	59.3	97.8	91.4	0.84
MOX23	13.7	41.2	78.9	96.4	1.57
ISE SL	11.2	32	6.7	80.5	78.33

DOI: https://doi.org/10.1515/helmin-2015-0002 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

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Language: English

Page range: 120 - 125

Submitted on: Sep 10, 2014

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Accepted on: Dec 10, 2015

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Published on: Apr 22, 2016

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

anthelmintic resistance,

Related subjects:

Life sciences, other,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2016 M. Urda Dolinská, A. Königová, M. Babják, M. Várady, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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