The Influence of Supplementation of Feed with Lactobacillus reuteri L2/6 Biocenol on Intestinal Microbiota of Conventional Mice

Nemcová, R.; Maďar, M.; Gancarčíková, S.; Pistl, J.

doi:10.1515/fv-2017-0024

Abstract

FISH (fluorescence in situ hybridization) analysis of the intestinal tract of conventional mice, following 14-day supplementation of feed with host non-specific (porcine) strain L. reuteri L2/6, showed in the presence of complex microbiota, a significant increase in the counts of representatives of the genera Lactobacillus and Bifidobacterium, and a significant decrease in the representatives of the genera Clostridium, Bacteroides and Enterobacteriaceae. At the same time, the supplemented strain stimulated the population of caecal lactobacilli of the species L. reuteri. These results demonstrated that the L. reuteri L2/6 colonised the jejunum, ileum and caecum and modulated the investigated intestinal microbiota.

References

1. Apostolou, E., Pelto, L., Kirjavainen, P. V., Isolauri, E., Salminen, S. J., Gibson, G. R., 2001: Differences in the gut bacterial flora of healthy and milk-hypersensitive adults, as measured by fluorescence in situ hybridization. FEMS Immunol. Med. Microbiol., 30, 217—221.10.1111/j.1574-695X.2001.tb01573.x11335141
Search in Google Scholar Back to article
2. Bermudez-Brito, M., Plaza-Díaz J., Muńoz-Quezada, S., Gómez-Llorente, C., Gil, A., 2012: Probiotic mechanisms of action. Ann. Nutr. Metab., 61, 160—174.10.1159/00034207923037511
Open DOI Search in Google Scholar Back to article
3. Bomba, A., Nemcová, R., Gancarčíková, S., Herich, R., Guba, P., Mudroňová, D., 2002: Improvement of the probiotic effect of microorganisms by their combination with maltodextrins, fructo-oligosacharides and polyunsatured fatty acids. Br. J. Nutr., 88, 95—99.10.1079/BJN200263412215187
Search in Google Scholar Back to article
4. Chang, Y. H., Kim, J. K., Kim, H. J., Kim, W. Y., Kim, Y. B., Park, Y. H., 2001: Selection of a potential probiotic Lactobacillus strain and subsequent in vivo studies. Antonie Van Leeuwenhoek, 80, 193—199.10.1023/A:1012213728917
Search in Google Scholar Back to article
5. Chiang, M. L., Chen, H. C., Chen, K. N., Lin, Y. C., Lin, Y. T., Chen, M. J., 2015: Optimizing production of two potential probiotic lactobacilli strains isolated from piglet faeces as feed additives for weaned piglets. Asian Australas. J. Anim. Sci., 28, 1163—1170.10.5713/ajas.14.0780447848526104525
Search in Google Scholar Back to article
6. Corcionivoschi, N., Drinceanu, D., Pop, I. M., Stack, D., Stef, L., Julean, C., Bourke, B., 2010: The effect of probiotics on animal health. Anim. Sci. Biotechnol., 43, 35—41.
Search in Google Scholar Back to article
7. Czerwiński, J., Højberg, O., Smulikowska, S., Engberg, R. M., Mieczkowska, A., 2012: Effects of sodium butyrate and salinomycin upon intestinal microbiota, mucosal morphology and performance of broiler chickens. Arch. Anim. Nutr., 66, 102—116.10.1080/1745039X.2012.66366822641923
Search in Google Scholar Back to article
8. De Vuyst, L., Vrancken, G., Ravyts, F., Rimaux, F., Weckx, S., 2009: Biodiversity, ecological determinants, and metabolic exploitation of sourdough microbiota. Food Microbiol., 26, 666—675.10.1016/j.fm.2009.07.01219747599
Search in Google Scholar Back to article
9. Eisenhauer, N., Scheu, S., Jousset, A., 2012: Bacterial diversity stabilizes community productivity. PLoS One. 7, e34517. doi: 10.1371/journal.pone.0034517.10.1371/journal.pone.0034517331463222470577
Search in Google Scholar Back to article
10. Franks, A. H., Harmsen, H. J., Raangs, G. C., Jansen, G. J., Schut, F., Welling, G. W., 1998: Variations of bacterial populations in human faeces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microb., 64, 3336—3345.10.1128/AEM.64.9.3336-3345.19981067309726880
Search in Google Scholar Back to article
11. Frese, S. A., Benson, A. K., Tannock, G. W., Loach, D. M., Kim, J., Zhang, M., et al., 2011: The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri. PLoS Genet., 7 (2. e1001314. doi: 10.1371/journal.pgen.1001314.10.1371/journal.pgen.1001314304067121379339
Search in Google Scholar Back to article
12. Frese, S. A., MacKenzie, D. A., Peterson, D. A., Schmaltz, R., Fangman, T., Zhou, Y., et al., 2013: Molecular characterization of host-specific biofilm formation in a vertebrate gut symbiont. PLoS Genet., 9, 1—13.10.1371/journal.pgen.1004057387325424385934
Search in Google Scholar Back to article
13. Fuentes, S., Egert, M., Jiménez-Valera, M., Ramos-Cormenzana, A., Ruiz-Bravo, A., Smidt, H., Monteoliva-Sanchez, M., 2008: Administration of Lactobacillus casei and Lactobacillus plantarum affects the diversity of murine intestinal lactobacilli, but not the overall bacterial community structure. Res. Microbiol., 159, 237—243.10.1016/j.resmic.2008.02.00518439805
Search in Google Scholar Back to article
14. Giang, H. H., Viet, T. Q., Ogle, B., Lindberg, J. E., 2010: Growth performance digestibility gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria. Livest. Sci., 129, 95—103.10.1016/j.livsci.2010.01.010
Search in Google Scholar Back to article
15. Harmsen, H. J. M., Elfferich, P., Schut, F., Welling, G. W., 1999: A 16S rRNA-targeted probe for detection of lactobacilli and enterococci in faecal samples by fluorescent in situ hybridization. Microbiol. Ecol. Health Dis., 11, 3—12.10.3402/mehd.v11i1.7876
Search in Google Scholar Back to article
16. Hou, Ch., Zeng, X., Yang, F., Liu, H., Qiao, S., 2015: Study and use of the probiotic Lactobacillus reuteri in pigs: a review. Journal of Animal Science and Biotechnology, 6:14 doi 10.1186/s40104-015-0014-3.10.1186/s40104-015-0014-3442358625954504
Open DOI Search in Google Scholar Back to article
17. Huang, C. H., Qiao, S. Y., Li, D. F., Piao, X. S., Ren, J. P., 2004: Effects of Lactobacilli on the performance diarrhoea incidence VFA concentration and gastrointestinal microbial flora of weaning pigs. Asian Aust. J. Anim. Sci., 17, 401—409.10.5713/ajas.2004.401
Search in Google Scholar Back to article
18. Jansen, G. J., Mooibroek, M., Idema, J., Harmsen, H. J., Welling, G. W., Degener, J. E., 2000: Rapid identification of bacteria in blood cultures by using fluorescently labelled oligonucleotide probes. J. Clin. Microbiol., 38, 814—817.10.1128/JCM.38.2.814-817.20008621110655390
Search in Google Scholar Back to article
19. Langendijk, P. S., Schut, F., Jansen, G. J., Raangs, G. C., Kamphuis, G. R., Wilkinson, M. H. F., Welling, G. W., 1995: Quantitative in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA targeted probes and its application in faecal samples. Appl. Environ. Microbiol., 61, 3069—3075.10.1128/aem.61.8.3069-3075.19951675847487040
Search in Google Scholar Back to article
20. Li, D., Ni, K., Pang, H., Wang, Y., Cai, Y., Jin, Q., 2015: Identification and antimicrobial activity detection of lactic acid bacteria isolated from corn stover silage. Asian Australas. J. Anim. Sci., 28, 620—631.10.5713/ajas.14.0439441299125924957
Search in Google Scholar Back to article
21. Liu, H., Zhang, J., Zhang, S. H., Yang, F. J., Thacker, P. A., Zhang, G. L., et al., 2014: Oral administration of Lactobacillus fermentum I5007 favours intestinal development and alters the intestinal microbiota in formula-fed piglets. J. Agric. Food. Chem., 62, 860—866.10.1021/jf403288r24404892
Search in Google Scholar Back to article
22. Oh, P. L., Benson, A. K., Peterson, D. A., Patil, P. B., Moriyama, E. N., Roos, S., Walter, J., 2010: Diversification of the gut symbiont Lactobacillus reuteri as a result of host-driven evolution. ISME J., 4, 377—387.10.1038/ismej.2009.12319924154
Open DOI Search in Google Scholar Back to article
23. Preidis, G., Saulnier, D., Blutt, S., Mistretta, T., Riehle, K., Major, A., et al., 2012: Probiotics stimulate enterocyte migration and microbial diversity in the neonatal mouse intestine. FASEB J., 26, 1960—1969.10.1096/fj.10-177980333678522267340
Open DOI Search in Google Scholar Back to article
24. Preidis, G., Versalovic, J., 2009: Targeting the human microbiome with antibiotics, probiotics, and prebiotics: gastroenterology enters the metagenomics era. Gastroenterol., 136, 2015—2031.10.1053/j.gastro.2009.01.072410828919462507
Search in Google Scholar Back to article
25. Quevedo, B., Giertsen, E., Zijnge, V., Lüthi-Schaller, H., Guggenheim, B., Thurnheer, T., Gmür, R., 2011: Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms. BMC Microbiol., 11:14. doi: 10.1186/1471-2180-11-4.10.1186/1471-2180-11-4302264421208463
Open DOI Search in Google Scholar Back to article
26. Ryznerová, D., 2013:The Study of Properties of Probiotic Bacteria from the Point of View of their Biological Effects and Application (In Slovak). Dissertation thesis, University of Veterinary Medicine and Pharmacy, Košice, Slovakia, 146 pp.
Search in Google Scholar Back to article
27. Slížová, M., Nemcová, R., Maďar, M., Hádryová, J., Gancarčíková, S., Popper, M., Pistl, J., 2015: Analysis of biofilm formation by intestinal lactobacilli. Can. J. Microbiol., 61, 437—446.10.1139/cjm-2015-000725961850
Search in Google Scholar Back to article
28. Su, M. S., Phaik, L. O., Walter, J., Gänzle, M. G., 2012: Phylogenetic, genetic, and physiological analysis of sourdough isolates of Lactobacillus reuteri: food fermenting strains of intestinal origin. Appl. Environ. Microbiol., 78, 6777—6780.10.1128/AEM.01678-12342670122798372
Open DOI Search in Google Scholar Back to article
29. Thomas, C., Versalovic, J., 2010: Probiotics-host communication: modulation of signalling pathways in the intestine. Gut Microbes, 1, 148—163.10.4161/gmic.1.3.11712290949220672012
Search in Google Scholar Back to article
30. Thu, T. V., Loh, T. C., Foo, H. L., Yaakub, H., Bejo, M. H., 2011: Effects of liquid metabolite combinations produced by Lactobacillus plantarum on growth performance, faeces characteristics, intestinal morphology and diarrhoea incidence in post weaning piglets. Trop. Anim. Health. Prod., 43, 69—75.10.1007/s11250-010-9655-6299585920632092
Search in Google Scholar Back to article
31. Walter, J., 2008a: Ecological role of lactobacilli in the gastrointestinal tract: Implications for fundamental and biomedical research. Appl. Environ. Microbiol., 74, 4985—4996.10.1128/AEM.00753-08251928618539818
Open DOI Search in Google Scholar Back to article
32. Walter, J., Schwab, C., Loach, D. M., Gänzle, M. G., Tannock, G. W., 2008b: Glucosyltransferase A (GtfA) and inulosucrase (Inu) of Lactobacillus reuteri TMW1.106 contribute to cell aggregation, in vitro biofilm formation, and colonization of the mouse gastrointestinal tract. Microbiol., 154, 72—80.10.1099/mic.0.2007/010637-018174127
Search in Google Scholar Back to article

The Influence of Supplementation of Feed with Lactobacillus reuteri L2/6 Biocenol on Intestinal Microbiota of Conventional Mice

Abstract

Paradigm

My account