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Investigation of Biosensor Potential Component Stability Caused by Influence of External Condition Cover

Investigation of Biosensor Potential Component Stability Caused by Influence of External Condition

Open Access
|Jan 2020

Abstract

The analysis of UV-VIS spectrum was used for testing changes through 36 days and the impact of external conditions (reduced temperature (R), microwave radiation (M) and normal conditions (NC) for comparisons) on the stability of: BSA and BSA-Fe2+ complexes after different treating procedure. The increase of BSA absorption with increasing concentration of Fe2+ was observed. Increased absorption of BSA, and BSA-Fe2+ with time, related probably to conformational changes present in the protein and changes in electrostatic reactions within the BSA-Fe2+ complexes. Examination of the impact of an external factor on stabilization of the protein and complexes showed a tendency to keep the stability under reduced temperature and a trend with accelerated protein and complex aging under microwave radiation. The observed tendencies to changes under the influence of external factors became more significant in time. These effects were most probably related to changes of the protein structure and time, while the observed trend of accelerating the changes was impacted by the applied external factors.

DOI: https://doi.org/10.1515/eces-2019-0048 | Journal eISSN: 2084-4549 | Journal ISSN: 1898-6196
Language: English
Page range: 665 - 674
Published on: Jan 21, 2020
Published by: Society of Ecological Chemistry and Engineering
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:

© 2020 Aleksandra Kłos-Witkowska, Vasyl Martsenyuk, published by Society of Ecological Chemistry and Engineering
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.