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Content of TPCs, TFCs, FC and TTCs of Cannabis extracts
| TPC (mg GAE/g DWE) | TFC (mg QE/g DW) | FC (mg QE/g DW) | TTC (mg CE/g DW) | |
|---|---|---|---|---|
| CHL-CBD | 130 ± 2.5a | 6 ± 1.2a | 0.9 ± 0.2a | 0.46 ± 0.09a |
| HEX-THC | 175 ± 4b | 14 ± 1.5b | 2.2 ± 0.4b | 0.51 ± 0.08a |
Cannabinoid profile of Cannabis sativa L_ extracts and their percentage area
| Molecular weight | Molecular structure | Retention time | Area % | ||
|---|---|---|---|---|---|
| HEX-THC (%) | CHL -CBD (%) | ||||
| CBD | 314 |
| 22.86 | <1 | 47.38 |
| THC | 314 |
| 23.91 | 89.87 | <1 |
Identified compounds in cannabis sativa L_ HEX-THC and CHL-CBD extracts using HPLC-DAD
| Pick number | Retention time | Compound | Area % | |
|---|---|---|---|---|
| HEX-THC | CHL-CBD | |||
| 1 | 3.35 |
| 2.2 | 0.5 |
| 2 | 5.45 | Gallic acid | 2.1 | 1.9 |
| 3 | 8.50 | Catechin hydrate | 1 | 0.8 |
| 4 | 14.68 | P-coumaric acid | 4 | 7.2 |
| 5 | 30.38 | Quercetin | 30 | 29.1 |
| 6 | 31.60 | Rosmarinic acid | 0.1 | 0.4 |
Membrane stabilizing effect of HEX-THC and CHL-CBD on hypotonicity‑induced hemolysis test and heat‑induced hemolysis test
| IC50 heat-induced test (μg/mL) | IC50 hypotonicity-induced test (μg/mL) | |
|---|---|---|
| CHL-CBD | 301.55 ± 8.43a | 469.58 ± 27.01a |
| HEX-THC | 185.94 ± 8.81b | 264.51 ± 41.18b |
| Aspirin | 100.73 ± 4.60c | — |
| Indomethacin | — | 121.63 ± 6.75c |
Antioxidant potential of Cannabis sativa L_ extracts was assessed using DPPH, ABTS, TAC and FRAP assays
| DPPH (IC50 μg/mL) | ABTS (IC50 μg/mL) | TAC μg AAE/g DWE | FRAP (EC50 μg/mL) | |
|---|---|---|---|---|
| CHL-CBD | 45 ± 2.7a | 65 ± 5a | 88 ± 3a | 33 ± 2a |
| HEX-THC | 32 ± 43b | 47 ± 4.1b | 108 ± 4b | 26 ± 1.9b |
| BHT | 14.1 ± 1c | — | — | — |
| Ascorbic acid | — | 12.34c | — | 55.4c |
Interacting amino acids, types of interactions, and binding affinity of ligands with various target proteins
| Ligands (from GC-MS and HPLC) | NF-κB | COX-1 | COX-2 | Lipoxygenase |
|---|---|---|---|---|
| 5LDE | 1EQG | 1CX2 | 3V99 | |
| Co-crystallized ligand | — | −7.6 | −9.2 | −6.0 |
| CBD | −4.6 | −6.9 | −7.7 | −7.4 |
| THC | −5.2 | −7.3 | −8.7 | −7.8 |
|
| −4.0 | −6.1 | −6.2 | −5.8 |
| Gallic acid | −3.7 | −6.6 | −6.7 | −6.3 |
| (+)-catechin hydrate | −4.8 | −7.1 | −8.4 | −8.0 |
| P-coumaric acid | −4.5 | −6.2 | −7.0 | −6.4 |
| Quercetin | −5.6 | −8.9 | −9.6 | −8.6 |
| Rosmarinic acid | −3.7 | −6.8 | −9.2 | −7.4 |